当前位置： SCI文献检索 > BIOCHEMICAL PHARMACOLOGY期刊下所有文献 > HeLa cell RNA and protein syntheses. Effects of long-term treatment with 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB).

HeLa cell RNA and protein syntheses. Effects of long-term treatment with 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB).

Abstract：

:The population growth rate of HeLa cells treated with 60 microM 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) in reinforced Eagle's minimum essential medium was directly related to the concentration of fetal bovine serum in the range from 5 to 30%. Control cells proliferated at a maximal rate (doubling time: 18-19 hr) at serum concentrations in this range. In 60 microM DRB-containing medium supplemented with 5% serum, the inhibition of hnRNA synthesis after 1,5, and 48 hr of treatment was equivalent to 78, 84, and 51%, respectively, whereas that of protein synthesis was equivalent to 5, 41, and 49%. In 15% serum, the inhibition of hnRNA synthesis after 1, 5, and 48 hr of treatment was equivalent to 75, 79, and 40%, whereas that of protein synthesis was equivalent to 13, 30, and 21%. Thus, after 48 hr of treatment of cells with DRB in either 5 or 15% serum, hnRNA synthesis was less inhibited than it was after 1 or 5 hr of treatment, and the level of protein synthesis in DRB-treated cells did not decrease between the 5th and 48th hr. After 48 hr of treatment of cells with DRB in 15% serum, hnRNA and protein syntheses were both somewhat less inhibited than after treatment in 5% serum. These results suggest that, on prolonged treatment, HeLa cells undergo a partial, serum-dependent adaptation to the inhibitor.

journal_name

Biochem Pharmacol

journal_title

Biochemical pharmacology

authors

Tamm I

doi

10.1016/0006-2952(84)90306-x

subject

Has Abstract

pub_date

1984-02-15 00:00:00

pages

551-7

issue

4

eissn

0006-2952

issn

1873-2968

pii

0006-2952(84)90306-X

journal_volume

33

pub_type

杂志文章

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