Reliable identification of herpes simplex viruses by DNA restriction endonuclease analysis with EcoRI.

Abstract:

:A simple procedure that permits the rapid identification of clinical isolates of herpes simplex virus (HSV, type 1 or type 2) on the basis of stable and type-specific differences in the EcoRI restriction endonuclease patterns of the HSV-1 and HSV-2 genomes was tested for reliability. Complete concordance was first obtained for 298 HSV isolates typed by standard laboratory procedures and by analysis of the DNA profiles. Two other HSV isolates showing intermediate biologic, immunologic, and/or biochemical properties were unambiguously identified by restriction endonuclease analysis of their genomes. Identical diagnostic results were also obtained by independent laboratories for another series of 150 HSV isolates after analysis of the EcoRI digestion products. As compared with other restriction enzymes, little intratypic variation in the DNA fragments selected for immediate identification of the isolates was found with EcoRI. The procedure used here, which does not require the purification and/or the radiolabeling of viral DNA, thus appears as most appropriate for the unambiguous typing of large numbers of HSV clinical isolates.

journal_name

Sex Transm Dis

authors

Gerson M,Portnoy J,Hamelin C

doi

10.1097/00007435-198404000-00007

subject

Has Abstract

pub_date

1984-04-01 00:00:00

pages

85-90

issue

2

eissn

0148-5717

issn

1537-4521

journal_volume

11

pub_type

杂志文章
  • Diagnostic considerations in intra-amniotic syphilis.

    abstract::Amniotic fluid is rarely used in the evaluation of syphilis during pregnancy but was available from a woman with positive serologic tests in the 30th week of gestation. Nonmotile intra-amniotic spirochetes were seen by dark-field microscopy 20 hr after treatment with benzathine penicillin G. The identity of the organi...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/00007435-198507000-00010

    authors: Glover DD,Winter CA,Charles D,Larsen B

    更新日期:1985-07-01 00:00:00

  • Papillomavirus infection among abortion applicants and patients at a sexually transmitted disease clinic.

    abstract::The base-line prevalence of human papillomavirus (HPV) infection among applicants for first trimester induced abortion and among a sexually transmitted disease (STD) clinic population with macroscopically visible condyloma is investigated. Cervical cells were collected from 505 women applying for induced abortion. Cel...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:

    authors: Csángó PA,Skuland J,Nilsen A,Pedersen BS,Jagars G

    更新日期:1992-05-01 00:00:00

  • Cocaine use and high-risk sexual behavior among STD clinic patients.

    abstract:BACKGROUND AND OBJECTIVES:Crack-smoking sexually transmitted disease (STD) patients are at high-risk for contracting HIV. GOAL OF THE STUDY:To examine the effects of cocaine use and other correlates on high-risk sexual behavior among STD clinic patients. STUDY DESIGN:This was a cross-sectional study of 1,490 consecut...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/00007435-199902000-00003

    authors: Hser YI,Chou CP,Hoffman V,Anglin MD

    更新日期:1999-02-01 00:00:00

  • A prospective study of risk factors for bacterial vaginosis in HIV-1-seronegative African women.

    abstract:BACKGROUND:Bacterial vaginosis (BV) is common and has been associated with increased HIV-1 susceptibility. The objective of this study was to identify risk factors for BV in African women at high risk for acquiring HIV-1. METHODS:We conducted a prospective study among 151 HIV-1-seronegative Kenyan female sex workers. ...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/OLQ.0b013e31816907fa

    authors: McClelland RS,Richardson BA,Graham SM,Masese LN,Gitau R,Lavreys L,Mandaliya K,Jaoko W,Baeten JM,Ndinya-Achola JO

    更新日期:2008-06-01 00:00:00

  • Agreement in reported sexual partnership dates and implications for measuring concurrency.

    abstract:OBJECTIVES:To describe the reliability of reported dates of first and last sexual exposure, as elicited from sexually transmitted disease/human immunodeficiency virus cases during routine contact investigation, and determine their adequacy for assessing concurrency. METHODS:We used contact tracing data from 5 studies ...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/01.olq.0000204746.58160.c1

    authors: Brewer DD,Rothenberg RB,Muth SQ,Roberts JM Jr,Potterat JJ

    更新日期:2006-05-01 00:00:00

  • Chlamydial infections in Italy.

    abstract::Chlamydia trachomatis was present in cell cultures of specimens from 96 (32%) of 300 men with nongonococcal urethritis, but this organism was not isolated from 100 healthy volunteers matched for sex and age. The prevalence of serum antibodies to C. trachomatis was investigated with the use of a single-antigen, indirec...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:

    authors: Cevenini R,La Placa M

    更新日期:1981-10-01 00:00:00

  • Differences between nonnational and indigenous patients with sexually transmitted infections in Italy and insight into the control of sexually transmitted infections.

    abstract:BACKGROUND:To determine the health needs non-nationals, information on disease occurrence is fundamental. GOALS The goal of this study was to assess the clinical and behavioral characteristics of non-Italians with a new sexually transmitted infection (STI). METHODS:Data were taken from Italy's National STI Surveillanc...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/01.OLQ.0000109975.74152.29

    authors: Giuliani M,Suligoi B,Italian STI Surveillance Working Group.

    更新日期:2004-02-01 00:00:00

  • High prevalence and incidence of asymptomatic sexually transmitted infections during pregnancy and postdelivery in KwaZulu Natal, South Africa.

    abstract:BACKGROUND:We report the prevalence and incidence of 3 treatable sexually transmitted pathogens (Neiserria gonorrhoeae, Chlamydia trachomatis, and Trichomonas vaginalis) in women who were HIV infected or at high risk for HIV infection, in pregnancy and postpartum, respectively. METHOD:Vulvovaginal specimens collected ...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/OLQ.0000000000000219

    authors: Moodley D,Moodley P,Sebitloane M,Soowamber D,McNaughton-Reyes HL,Groves AK,Maman S

    更新日期:2015-01-01 00:00:00

  • Stay in school? Results of a sexually transmitted diseases screening program in San Francisco high schools-2007.

    abstract:OBJECTIVES:To provide chlamydia and gonorrhea screening and treatment to adolescents presumed to be at high risk, school screening was conducted among the 11th and 12th graders in San Francisco. STUDY DESIGN:Two schools in neighborhoods with high chlamydia and gonorrhea rates and student populations > or = 15% black w...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/OLQ.0b013e31816a43d3

    authors: Barry PM,Scott KC,McCright J,Snell A,Lee M,Bascom T,Kent CK,Klausner JD

    更新日期:2008-06-01 00:00:00

  • HIV transmission risk behavior and its relation to antiretroviral treatment adherence.

    abstract:BACKGROUND:Rising rates of antiretroviral drug resistance among recently infected persons are evidence of high-risk behavior among persons in HIV care. GOAL:The goal was to determine HIV transmission risk behavior among persons in HIV care and evaluate its association with treatment adherence. STUDY DESIGN:The study ...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/00007435-200305000-00005

    authors: Flaks RC,Burman WJ,Gourley PJ,Rietmeijer CA,Cohn DL

    更新日期:2003-05-01 00:00:00

  • Self-reported symptoms of reproductive tract infections among rural women in Hainan, China: prevalence rates and risk factors.

    abstract:OBJECTIVES AND GOAL:The goal of this study was to examine risk of reproductive tract infections (RTIs) among women in rural China, including prevalence, influence of sociodemographic characteristics, knowledge, hygienic practices, attitudes, and behaviors related to premarital and extramarital sex, and abortion. STUDY...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/01.olq.0000143111.33741.40

    authors: Xia DY,Liao SS,He QY,Choi KH,Mandel JS

    更新日期:2004-11-01 00:00:00

  • Factors associated with condom use problems during vaginal sex with main and non-main partners.

    abstract:BACKGROUND:Incorrect condom use is a common problem that can undermine their prevention impact. We assessed the prevalence of 2 condom use problems, breakage/slippage and partial use, compared problems by partnership type, and examined associations with respondent, partner, and partnership characteristics. METHODS:Dat...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章,多中心研究

    doi:10.1097/OLQ.0b013e31825ef325

    authors: DʼAnna LH,Korosteleva O,Warner L,Douglas J,Paul S,Metcalf C,McIlvaine E,Malotte CK,RESPECT-2 Study Group.

    更新日期:2012-09-01 00:00:00

  • Sexual behavior risk factors associated with bacterial vaginosis and Chlamydia trachomatis infection.

    abstract:BACKGROUND AND OBJECTIVES:Few studies have demonstrated that bacterial vaginosis (BV) is associated with sexual behavior risk factors similar to those for other sexually transmitted diseases. The objective of this study was to determine the prevalence of these factors in a multivariate analysis of data from women infec...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/00007435-199705000-00001

    authors: Nilsson U,Hellberg D,Shoubnikova M,Nilsson S,Mårdh PA

    更新日期:1997-05-01 00:00:00

  • Sexually transmitted diseases and tubal pregnancy.

    abstract::The risk of tubal pregnancy associated with seropositivity to Chlamydia trachomatis or following a diagnosis of gonorrhea, genital herpes, trichomoniasis, genital warts, or nonspecific vaginal infection was evaluated in a study of this condition among women who were members of a prepaid health plan. A total of 264 wom...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/00007435-199007000-00001

    authors: Sherman KJ,Daling JR,Stergachis A,Weiss NS,Foy HM,Wang SP,Grayston JT

    更新日期:1990-07-01 00:00:00

  • Neighborhoods at risk: estimating risk of higher Neisseria gonorrhoeae incidence among women at the census tract level.

    abstract:BACKGROUND:The association between area-based social factors and sexually transmitted diseases has been demonstrated in numerous studies. Such associations have not previously been explored for their potential to quantify likelihood of higher transmission of gonorrhea in small geographic areas. METHODS:Aggregate censu...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章,多中心研究

    doi:10.1097/OLQ.0000000000000195

    authors: Stenger MR,Samuel MC,Anschuetz GL,Pugsley R,Eaglin M,Klingler E,Reed M,Schumacher CM,Simon J,Weinstock H

    更新日期:2014-11-01 00:00:00

  • Low prevalence of ciprofloxacin-resistant Neisseria gonorrhoeae in Nuuk, Greenland.

    abstract::During a 35-day period, 32 isolates of Neisseria gonorrhoeae strains were collected by culture from patients with positive nucleic acid amplification test results in Nuuk, Greenland, where ciprofloxacin is the preferred treatment. All isolates were quinolone susceptible, suggesting that resistance is rare. Sampling pa...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/01.OLQ.0000430780.79488.0f

    authors: Pedersen ML,Clausen-Dichow P,Poulsen P,Nyborg H,Jensen JS

    更新日期:2013-08-01 00:00:00

  • Reduction in sexual risk behaviors and infection rates among African Americans and Mexican Americans.

    abstract:BACKGROUND:Project SAFE, a gender- and culture-specific cognitive-behavioral intervention, was one of the few interventions to have demonstrated a significant reduction in sexually transmitted infections in a randomized, controlled trial. GOAL:We evaluated intervention efficacy in 379 Mexican Americans and 170 African...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/01.olq.0000115461.50698.55

    authors: Korte JE,Shain RN,Holden AE,Piper JM,Perdue ST,Champion JD,Sterneckert K

    更新日期:2004-03-01 00:00:00

  • Oral Human Papillomavirus in Youth From the Pediatric HIV/AIDS Cohort Study.

    abstract::In contrast to high rates of oral human papillomavirus (HPV) found in human immunodeficiency virus (HIV)-infected adults, only 2% of 209 perinatally HIV-infected youth had oral HPV. This rate was similar in HIV-exposed but uninfected youth. No association was found with sexual activity; however, low CD4 counts were as...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/OLQ.0000000000000495

    authors: Moscicki AB,Farhat S,Yao TJ,Ryder MI,Russell JS,Van Dyke RB,Hazra R,Shiboski CH,Pediatric HIVAIDS Cohort Study.

    更新日期:2016-08-01 00:00:00

  • Detection of human papillomavirus DNA in the urogenital tracts of men with anogenital warts.

    abstract::Increasing evidence suggests that human papillomavirus (HPV) infection of the female anogenital tract is multifocal. Less is known of the distribution of HPVs in men. To investigate this, a prospective study was conducted of 116 men consecutively attending a clinic for ablative treatment of anogenital warts. Wart tiss...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/00007435-199301000-00005

    authors: Hillman RJ,Botcherby M,Ryait BK,Hanna N,Taylor-Robinson D

    更新日期:1993-01-01 00:00:00

  • Incidence of disseminated gonococcal infection correlated with the presence of AHU auxotype of Neisseria gonorrhoeae in a community.

    abstract::Strains of Neisseria gonorrhoeae requiring arginine, hypoxanthine, and uracil (AHU) may cause disseminated gonococcal infection (DGI). A significant epidemiologic association was noted between the incidence of disseminated gonococcal infection and the presence of gonococci of the AHU auxotype in the community over a n...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/00007435-198404000-00003

    authors: Noble RC,Reyes RR,Parekh MC,Haley JV

    更新日期:1984-04-01 00:00:00

  • Hepatitis A and B vaccination in a sexually transmitted disease clinic for men who have sex with men.

    abstract:BACKGROUND:Sexually transmitted disease clinics can deliver hepatitis vaccines to men who have sex with men, but have been reluctant to do so because of perceived low vaccination completion rates. GOAL:The goal was to evaluate hepatitis A and B vaccination eligibility, acceptance, and completion and the effectiveness ...

    journal_title:Sexually transmitted diseases

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验

    doi:10.1097/01.OLQ.0000079524.04451.4C

    authors: Sansom S,Rudy E,Strine T,Douglas W

    更新日期:2003-09-01 00:00:00

  • Characterization of ciprofloxacin resistance in Neisseria gonorrhoeae isolates in Canada.

    abstract:BACKGROUND:Ciprofloxacin (500 mg orally, single dose) is one of the recommended therapies for gonorrhea in Canada. In Canada, the first ciprofloxacin-resistant (CipR) Neisseria gonorrhoeae strain was isolated in 1993. Antimicrobial susceptibilities of N gonorrhoeae isolates were monitored as part of a national surveill...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章,多中心研究

    doi:10.1097/00007435-200212000-00008

    authors: Ng LK,Sawatzky P,Martin IE,Booth S

    更新日期:2002-12-01 00:00:00

  • Emergency care of urban women with sexually transmitted infections: time to address deficiencies.

    abstract:BACKGROUND:Patient education upon diagnosis of a sexually transmitted infection (STI) may effect changes in high-risk sexual behavior. OBJECTIVE:Describe emergency department (ED) communication with urban female patients treated for STIs. METHODS:: This secondary analysis of data collected during a study of ED commun...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/OLQ.0b013e318188389b

    authors: Rhodes KV,Bisgaier J,Becker N,Padowitz N,Vashi A,McNutt LA

    更新日期:2009-01-01 00:00:00

  • Trends in sexual behavior, testing, and knowledge in young people; 2006-2011.

    abstract::Cross-sectional surveys were conducted annually from 2006 to 2011 at a music festival. Eight thousand one hundred sixty-five young people completed surveys. STI testing rates increased over time, but there was an increase in the prevalence of some sexual risk behaviors and little improvement in STI knowledge between 2...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/OLQ.0b013e3182663f27

    authors: Lim MS,Bowring AL,Gold J,Aitken CK,Hellard ME

    更新日期:2012-11-01 00:00:00

  • Sexual Risk Factors and Human Immunodeficiency Virus Testing Intention Among At-Risk College Students Who Have Never Been Tested.

    abstract::Survey data from a university in the southeast United States were used to estimate the relation between sexual risk factors, perceived susceptibility, and human immunodeficiency virus testing intention among sexually active college students who had never been tested. Sexual risk factors, but not knowledge, were indire...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/OLQ.0000000000001000

    authors: James TG,Cheong J,Ryan SJ

    更新日期:2019-07-01 00:00:00

  • A novel method to increase the viability of Trichomonas vaginalis in urine.

    abstract:OBJECTIVES:Two of the major diagnostic methods for Trichomonas vaginalis, wet mount and culture, rely on the continued viability of the organism. Methods to increase the viability of T. vaginalis in urine are needed. GOAL:The goal of this study was to develop a method that increases the time of viability of T. vaginal...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/01.olq.0000249724.72816.a0

    authors: Shafir SC,Sorvillo FJ,Upcroft JA,Upcroft P

    更新日期:2007-07-01 00:00:00

  • Concurrent sexual partnerships and sexually transmitted diseases in Russia.

    abstract:BACKGROUND:Sexual concurrency is associated with higher prevalence of sexually transmitted diseases (STDs) including HIV. However, most studies have focused only on the concurrency of the individual participant (individual concurrency) and not on concurrency of their sexual partners (partner concurrency). Furthermore, ...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/OLQ.0b013e318205e449

    authors: Zhan W,Krasnoselskikh TV,Niccolai LM,Golovanov S,Kozlov AP,Abdala N

    更新日期:2011-06-01 00:00:00

  • A solid-phase enzyme immunoassay (Gonozyme) test for direct detection of Neisseria gonorrhoeae antigen in urogenital specimens from patients at a sexually transmitted disease clinic.

    abstract::The Gonozyme test (Abbott Laboratories; North Chicago, IL), a solid-phase enzyme immunoassay (EIA), was evaluated for direct detection of antigens from Neisseria gonorrhoeae in clinical specimens. Results of the EIA were compared with those of culture and gram stain. Separate swab specimens for each procedure were col...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/00007435-198510000-00006

    authors: Nasello MA,Callihan DR,Menegus MA,Steigbigel RT

    更新日期:1985-10-01 00:00:00

  • HIV/AIDS behavioral surveillance surveys of the cross-border sex-networker population in Hong Kong from 1997 to 2001.

    abstract:BACKGROUND:Mobility is an important risk factor in the spread of HIV. GOAL:The goal was to conduct behavioral surveillance surveys on Hong Kong males crossing the border to mainland China to have sex with female sex workers (FSW) or nonregular female sex partners who were not FSW (NRP). STUDY DESIGN:Four surveys (199...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/01.OLQ.0000086605.65963.12

    authors: Lau JT,Tsui HY

    更新日期:2003-11-01 00:00:00

  • Neurocognitive impairment in human immunodeficiency virus infection is correlated with sexually transmitted disease history.

    abstract:BACKGROUND AND OBJECTIVES:Neurocognitive impairment is common in human immunodeficiency virus (HIV)-infected subjects. The relationship of sexually transmitted diseases to neurocognitive changes is unknown. GOAL:To establish whether HIV-infected patients with a history of syphilis or gonorrhea have a higher rate of ne...

    journal_title:Sexually transmitted diseases

    pub_type: 杂志文章

    doi:10.1097/00007435-199708000-00003

    authors: Wallace MR,Heaton RK,McCutchan JA,Malone JL,Velin R,Nelson J,Miller LK,Weiss PJ,Oldfield EC 3rd,Grant I

    更新日期:1997-08-01 00:00:00