Abstract：
:The role of subendothelial fibronectin in platelet interaction with subendothelium was studied. Human umbilical artery subendothelium was exposed to flowing blood containing 111In-labeled platelets in an annular perfusion chamber. Platelet adhesion was determined from the 111In radioactivity on the vessel wall. When perfusions were performed for five minutes at a wall shear rate of 1,800 s-1, platelet adhesion was the same whether normal plasma or fibronectin-free plasma was used. Preincubation of subendothelium with rabbit anti-human fibronectin serum, however, resulted in a marked inhibition of platelet adhesion. Preincubation with normal rabbit serum had no effect. Platelet adhesion was also diminished when the vessel wall was preincubated with anti-fibronectin IgG fraction or F(ab')2 fragment. After the latter preincubations, frozen sections of 4 micron were incubated with fluorescein isothiocyanate-conjugated goat anti-rabbit IgG, F(ab')2 fragment specific. Fluorescence was seen throughout the subendothelium both before and after perfusion. No fluorescence was seen when subendothelium was preincubated with normal rabbit IgG or F(ab')2 or with anti-fibronectin IgG that had been absorbed with purified fibronectin. After absorption of anti-fibronectin IgG with purified fibronectin, the inhibiting effect on platelet adhesion was also no longer present. Preincubation of the vessel wall with anti-fibronectin IgG reduced platelet adhesion significantly at a wall shear rate of 800 s-1. This effect was even greater at 1,800 s-1. At low shear rate (400 s-1), there was no inhibition.
journal_name
Bloodjournal_title
Bloodauthors
Houdijk WP,Sixma JJsubject
Has Abstractpub_date
1985-03-01 00:00:00pages
598-604issue
3eissn
0006-4971issn
1528-0020journal_volume
65pub_type
杂志文章相关文献
BLOOD文献大全abstract：:Over the past 2 decades considerable evidence has accumulated on the association between hepatitis C virus (HCV) and hepatitis B virus (HBV) and several hematologic malignancies, most notably B-cell non-Hodgkin lymphoma (NHL). In this review we summarize this evidence, address possible mechanisms whereby hepatitis vir...
journal_title：Blood
pub_type： 杂志文章,评审
doi：10.1182/blood-2010-06-275818
更新日期：2011-02-10 00:00:00
abstract：:The spread of multiple myeloma (MM) involves (re)circulation into the peripheral blood and (re)entrance or homing of MM cells into new sites of the BM. Hypoxia in solid tumors was shown to promote metastasis through activation of proteins involved in the epithelial-mesenchymal transition (EMT) process. We hypothesized...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2011-09-380410
更新日期：2012-06-14 00:00:00
abstract：:Lymphomas in 10 cynomolgus monkeys infected with a simian immunodeficiency virus (SIVsm) were studied with regard to proliferative activity and apoptosis-related gene expression. All were diffuse large-cell lymphomas, showed mono or oligoclonality and a 9/10 diploid cellular DNA content. Expression of a simian homolog...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1999-02-15 00:00:00
abstract：:A series of alterations in the cellular genome affecting the expression or function of genes controlling cell growth and differentiation is considered to be the main cause of cancer. These mutational events include activation of oncogenes and inactivation of tumor suppressor genes. The elucidation of human cancer at t...
journal_title：Blood
pub_type： 杂志文章,评审
doi：
更新日期：2000-09-01 00:00:00
abstract：:Using the technique of differential cDNA library screening, we have molecularly cloned a gene that is highly expressed in an undifferentiated myeloid multipotent and growth factor-dependent stem cell line (FDCP-Mix) and that downregulates as these cells are induced to differentiate along monocytic, granulocytic, and e...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1992-12-15 00:00:00
abstract：:The majority of mouse Vα14 invariant natural killer T (Vα14i NKT) cells produce several cytokines, including IFNγ and IL-4, very rapidly after activation. A subset of these cells, known as NKT17 cells, however, differentiates in the thymus to preferentially produce IL-17. Here, we show that the transcription factor-kn...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2012-01-406280
更新日期：2012-11-29 00:00:00
abstract：:Soluble thrombomodulin plasma concentrations are elevated in steroid-resistant graft-versus-host disease (GVHD), implying endothelial hypofunctioning for thrombomodulin-dependent generation of activated protein C's (APC) anticoagulant, anti-inflammatory, and antiapoptotic functions. Recombinant thrombomodulin or APC a...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood.2019001259
更新日期：2019-08-29 00:00:00
abstract：:Aneuploidy and lg light chain restriction were used as separate, independent tumor specific markers to study 26 patients with multiple myeloma to determine whether bone marrow B cells, as defined by CD19 expression, are clonally related to myeloma plasma cells. Specimens were characterized using multidimensional flow ...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1996-07-15 00:00:00
abstract：:The replating capability of human umbilical cord blood (CB) multipotential (CFU-GEMM) progenitors was assessed in vitro as an estimate of self-renewal using erythropoietin (Epo), steel factor (SLF), and either fetal bovine serum (FBS) or CB plasma. This study found a much higher replating efficiency for CB CFU-GEMM th...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1993-02-15 00:00:00
abstract：:We found an unusual fc gamma receptor IIIa (CD16) phenotype on the natural killer (NK) cells of a 3-year-old boy, who suffered from recurrent viral respiratory tract infections since birth. He also had severe clinical problems after Bacille Calmette-Geérin (BCG) vaccination and following Epstein-Barr virus and Varicel...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1996-10-15 00:00:00
abstract：:Over the past 5 years, many novel approaches to early diagnosis, prevention, and treatment of acute graft-versus-host disease (aGVHD) have been translated from the bench to the bedside. In this review, we highlight recent discoveries in the context of current aGVHD care. The most significant innovations that have alre...
journal_title：Blood
pub_type： 杂志文章,评审
doi：10.1182/blood-2014-01-514786
更新日期：2014-07-17 00:00:00
abstract：:In this issue of Blood, Mayer et al describe a new method of generating high numbers of Batf3- and Irf8-dependent CD103+ conventional dendritic cells (cDCs), providing new opportunities to study this subset of antigen-presenting cells specialized in crosspresentation. ...
journal_title：Blood
pub_type： 评论,杂志文章
doi：10.1182/blood-2014-08-595298
更新日期：2014-11-13 00:00:00
abstract：:Recent studies in humans have highlighted the importance of a distinct cellular entity, the plasmacytoid dendritic cell (PDC). To identify genes for which expression is restricted to human PDCs, a cDNA subtraction technique was applied using cDNA from activated monocyte-derived DCs (MDDCs) as competitor. In the 650 se...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2002-02-0638
更新日期：2002-11-01 00:00:00
abstract：:The outcome of patients with acute myeloid leukemia who are older than 60 years has remained poor because of unfavorable disease characteristics and patient-related factors. The randomized German-Austrian AML Study Group 06-04 protocol was designed on the basis of in vitro synergistic effects of valproic acid (VPA) an...
journal_title：Blood
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1182/blood-2013-12-546283
更新日期：2014-06-26 00:00:00
abstract：:High-dose chemoradiotherapy (HDT) with autologous stem cell transplantation (ASCT) is the treatment of choice for patients with relapsed aggressive non-Hodgkin lymphoma (NHL). However, its role in the treatment of patients with primary refractory disease is not well defined. The outcomes of 85 patients with primary re...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：2000-10-01 00:00:00
abstract：:The molecular mechanisms by which mesenchymal stem cells (MSCs) suppress T-cell proliferation are poorly understood, and whether a soluble factor plays a major role remains controversial. Here we demonstrate that the T-cell-receptor complex is not a target for the suppression, suggesting that downstream signals mediat...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2006-02-002246
更新日期：2007-01-01 00:00:00
abstract：:To assess the relationship between venous thrombosis and plasma glucosylceramide (GlcCer) or phosphatidylethanolamine (PE), plasma levels of GlcCer and PE were determined for 70 venous thrombosis patients referred for evaluation and 70 healthy blood donors. The mean GlcCer level, but not the PE level, was lower in pat...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood.v97.7.1907
更新日期：2001-04-01 00:00:00
abstract：:T cell-depleted haploidentical hematopoietic stem cell transplantation (haploHSCT) is an option to treat children with very high-risk acute lymphoblastic leukemia (ALL) lacking an HLA-identical donor. We analyzed 127 children with ALL who underwent haploHSCT in first (n = 22), second (n = 48), or third (n = 32), compl...
journal_title：Blood
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1182/blood-2009-03-207001
更新日期：2010-04-29 00:00:00
abstract：:Primary mediastinal large B-cell lymphoma (PMBCL) is a recognized non-Hodgkin lymphoma entity with unique pathologic, clinical, and molecular characteristics distinct from those of other diffuse large B-cell lymphomas. Immunohistochemical characterization and molecular studies strongly suggest that PMBCL is of germina...
journal_title：Blood
pub_type： 杂志文章,评审
doi：10.1182/blood-2011-05-326538
更新日期：2011-09-08 00:00:00
abstract：:Leukemia in infants is rare but generates tremendous interest due to its aggressive clinical presentation in a uniquely vulnerable host, its poor response to current therapies, and its fascinating biology. Increasingly, these biological insights are pointing the way toward novel therapeutic approaches. Using represent...
journal_title：Blood
pub_type： 杂志文章,随机对照试验
doi：10.1182/blood-2018-04-785980
更新日期：2019-01-17 00:00:00
abstract：:Measurement of plasma levels of two secreted platelet proteins (beta-thromboglobulin and platelet factor 4) has been suggested as a means for detecting increased platelet activation in vivo. A crucial question in the measurement is the distinction between in vivo and in vitro secretion of the proteins. One approach to...
journal_title：Blood
pub_type： 杂志文章,评审
doi：
更新日期：1981-02-01 00:00:00
abstract：:Erythroid cell iron and transferrin uptake and release was studied in the anemia of the Belgrade laboratory rat (gene symbol, b), an autosomal recessive trait characterized by hypochromia and hyperferrinemia. When reticulocyte-rich red cells were incubated in vitro with doubly (59Fe, 125I) labeled transferrin, b/b cel...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1980-04-01 00:00:00
abstract：:Meis1 is a homeodomain transcription factor coexpressed with Hoxa9 in most human acute myeloid leukemias (AMLs). In mouse models of leukemia produced by Hoxa9, Meis1 accelerates leukemogenesis. Because Hoxa9 immortalizes myeloid progenitors in the absence of Meis1 expression, the contribution of Meis1 toward leukemia ...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2004-12-4664
更新日期：2005-07-01 00:00:00
abstract：:Platelet endothelial cell adhesion molecule 1 (PECAM-1) is involved in leukocyte migration and angiogenesis, which are key components of venous thrombus resolution. This study investigated the effect of PECAM-1 deficiency on thrombus resolution in FVB/n mice and the extent to which levels of soluble PECAM-1 (sPECAM-1)...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2013-04-499558
更新日期：2013-11-07 00:00:00
abstract：:The human CD34 surface antigen is selectively expressed on stem/progenitor cells within the hematopoietic system. Because CD34 expression is tightly linked to the immature status of hematopoietic cells, with expression being rapidly lost as hematopoietic cells mature and differentiate, an examination of its regulation...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:The effects of immunoglobulin G (IgG) from patients with the antiphospholipid syndrome (APS) upon monocyte activation have not been fully characterized. We carried out a comprehensive proteomic analysis of human monocytes treated with IgG from patients with different manifestations of the APS. Using 2-dimensional diff...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2014-05-577569
更新日期：2014-12-11 00:00:00
abstract：:R115777 is a potent farnesyltransferase (FTase) inhibitor with substantial antitumor activity in preclinical models. We conducted a phase 1 study (3 + 3 design) of R115777 in patients with myelodysplastic syndrome (MDS). R115777 was administered twice daily (3-weeks-on/1-week-off schedule for 8 weeks) (starting dosage...
journal_title：Blood
pub_type： 临床试验,杂志文章
doi：10.1182/blood-2002-11-3359
更新日期：2003-12-15 00:00:00
abstract：:Studies of neutrophil nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activation in a cell-free system showed that the low molecular-weight guanosine triphosphatase (GTPase) Rac was required, and that Rap1a may participate in activation of the catalytic complex. Full-length posttranslationally modified Rac...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1995-02-01 00:00:00
abstract：:This phase 2 study evaluated the efficacy and safety of the oral farnesyltransferase inhibitor tipifarnib in adults with refractory or relapsed acute myeloid leukemia (AML). Patients (n=252) received tipifarnib 600 mg twice a day for 21 days in 28-day cycles. Median age was 62 years; 99 (39%) patients were 65 years or...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2006-09-046144
更新日期：2007-06-15 00:00:00
abstract：:Myeloid-derived suppressor cells (MDSCs) represent a heterogeneous population that shares certain characteristics including an aberrant myeloid phenotype and the ability to suppress T cells. MDSCs have been predominantly studied in malignant diseases and findings suggest involvement in tumor-associated immune suppress...
journal_title：Blood
pub_type： 临床试验,杂志文章
doi：10.1182/blood-2013-12-546416
更新日期：2014-07-31 00:00:00
