Abstract:
:Recent studies in humans have highlighted the importance of a distinct cellular entity, the plasmacytoid dendritic cell (PDC). To identify genes for which expression is restricted to human PDCs, a cDNA subtraction technique was applied using cDNA from activated monocyte-derived DCs (MDDCs) as competitor. In the 650 sequences analyzed, 25% were for B-cell transcripts. We also found lymphoid-related genes, immunoglobulinlike transcript 7 (ILT7), granzyme B (GrB), Spi-B, and the receptor tyrosine kinase Eph-B1. Granzyme B was up-regulated on activation, and protein was detected only in PDCs. Eph-B1 protein was expressed in the cytoplasm and the nuclei of PDCs and MDDCs, respectively. Interestingly, several novel molecules have been identified that were predicted to encode for a type 2 transmembrane protein (BRI(3)), a putative cytokine (C-15, a cysteine-rich-secreted protein), and a type 1 leucine-rich repeat protein (MAPA). The identification of genes expressed in PDCs provides new insights into their function and origin.
journal_name
Bloodjournal_title
Bloodauthors
Rissoan MC,Duhen T,Bridon JM,Bendriss-Vermare N,Péronne C,de Saint Vis B,Brière F,Bates EEdoi
10.1182/blood-2002-02-0638subject
Has Abstractpub_date
2002-11-01 00:00:00pages
3295-303issue
9eissn
0006-4971issn
1528-0020journal_volume
100pub_type
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