Abstract:
:MCF-7 human breast cancer cell homogenates and subcellular organelles were submitted to isopycnic centrifugation on Percoll gradients to investigate the subcellular localization of triphenylethylene antiestrogen specific binding sites (AEBS). Electron microscopy revealed that gradient fractions coincident with the migration of [3H]tamoxifen-AEBS complexes were homogeneously represented by rough and smooth endoplasmic reticulum. Eighty percent of AEBS were localized in the endoplasmic reticulum [45,000 +/- 4,000 sites/cell, mean +/- S.D.), while 20% of these sites were also found in the nuclear fraction (12,000 +/- 1,000 sites/cell, mean +/- S.D.). A similar subcellular distribution of AEBS was observed in human breast cancer bioptic specimens. No differences in [3H]tamoxifen binding affinity between microsomal and nuclear AEBS were observed in MCF-7 and bioptic breast cancer. No major differences in microsomal AEBS levels were observed in the limited number of estrogen receptor-positive or -negative breast cancer specimens we have studied, whereas estrogen receptor-negative samples had higher levels of nuclear AEBS with respect to estrogen receptor-positive tumors. The presence of AEBS was also detected in the human serum of healthy and tumor-bearing subjects. The affinity and the binding specificity of serum AEBS were similar to those of intracellular AEBS. No differences in the levels of serum AEBS were observed between healthy and tumor-bearing subjects [19 +/- 4 and 22 +/- 4 pmoles/ml (mean +/- S.D.) respectively. Human serum AEBS did not appear to be associated to lipoproteins, whereas it migrated as a 5.5 S sedimenting molecule.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Gulino A,Vacca A,Modesti A,Screpanti I,Farina A,Frati Ldoi
10.1016/0006-2952(86)90677-5subject
Has Abstractpub_date
1986-11-01 00:00:00pages
3863-70issue
21eissn
0006-2952issn
1873-2968pii
0006-2952(86)90677-5journal_volume
35pub_type
杂志文章abstract::The relative ease of generation of reactive oxygen species from a series of reductively activated aglycone and sugar modified anthracyclines was compared by the extents of single strand scission in supercoiled PM2-covalently closed circular (CCC)-DNA. The electrochemical properties of these agents were used as a quant...
journal_title:Biochemical pharmacology
pub_type: 杂志文章
doi:10.1016/0006-2952(82)90162-9
更新日期:1982-02-15 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
doi:10.1016/0006-2952(90)90360-w
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:1989-07-01 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
doi:10.1016/j.bcp.2005.05.029
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pub_type: 杂志文章
doi:10.1016/s0006-2952(97)82448-3
更新日期:1997-06-15 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
doi:10.1016/0006-2952(84)90521-5
更新日期:1984-04-01 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
doi:10.1016/0006-2952(93)90606-w
更新日期:1993-12-14 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:1996-08-23 00:00:00
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pub_type: 杂志文章
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更新日期:1990-06-15 00:00:00
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pub_type: 杂志文章
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
doi:10.1016/0006-2952(94)00404-a
更新日期:1995-01-06 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
doi:10.1016/0006-2952(91)90584-r
更新日期:1991-10-24 00:00:00
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pub_type: 杂志文章
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更新日期:2005-09-15 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:1994-09-15 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:2017-12-01 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:2020-12-02 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:1982-10-15 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
doi:10.1016/j.bcp.2006.05.021
更新日期:2006-08-28 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:1989-04-01 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:1985-08-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1997-10-15 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:2002-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0006-2952(85)90181-9
更新日期:1985-02-15 00:00:00
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pub_type: 杂志文章
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更新日期:1983-04-15 00:00:00
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pub_type: 杂志文章
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更新日期:1991-08-08 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
doi:10.1016/j.bcp.2010.03.020
更新日期:2010-07-15 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
doi:10.1016/j.bcp.2011.11.001
更新日期:2012-01-15 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
doi:10.1016/0006-2952(90)90037-l
更新日期:1990-01-15 00:00:00