Abstract:
:The partition coefficients (Kp) of three prototype Ca2+ antagonists, nitrendipine, (-)-desmethoxyverapamil and flunarizine were determined in native synaptic plasma membranes (SPM) isolated from sheep brain cortex and in liposomes prepared with the total lipids extracted from the membranes. We found that at 25 degrees and at 5 x 10(-6) M drug concentration the Kp values of the drugs for native SPM are higher than those obtained for liposomes, and are of the order of 334 +/- 53, 257 +/- 36 and 23 X 10(3) for nitrendipine, (-)desmethoxyverapamil and flunarizine, respectively, whereas the Kp values in liposomes are 190 +/- 41, 118 +/- 10 and 6 x 10(3) for the same drugs. The results suggest that the presence of membrane proteins favors the incorporation of the drugs in the membranes. Furthermore, the Kp values of the three Ca2+ antagonists studied increase with temperature in native membranes, but not in liposomes. It is concluded that the physical partitioning in membranes of drugs which act on Ca2+ channels may play some role in the mechanism of interaction of these drugs with the Ca2+ channel proteins.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Carvalho CM,Oliveira CR,Lima MP,Leysen JE,Carvalho APdoi
10.1016/0006-2952(89)90066-xsubject
Has Abstractpub_date
1989-07-01 00:00:00pages
2121-7issue
13eissn
0006-2952issn
1873-2968pii
0006-2952(89)90066-Xjournal_volume
38pub_type
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