Anti-morphine anti-idiotypic antibodies. Opiate receptor binding and isolated tissue responses.


:Anti-idiotypic antibodies which recognize the opiate receptor were generated in guinea pigs following immunization against purified rabbit anti-morphine antibodies. The anti-idiotypic antibodies produced a concentration-dependent inhibition of [3H]naloxone binding to opiate receptors in a membranous mouse brain preparation. Saturation analysis indicated that the antibodies produced a non-competitive inhibition of naloxone binding. The ability of the antibodies to interact with biological systems was investigated in in vitro systems. In both the isolated guinea pig ileal longitudinal muscle and mouse vas deferens, the antibodies produced a concentration-dependent, opiate agonist-like action. The anti-morphine anti-idiotypic antibodies appear to interact specifically with the opiate receptor and may serve as useful tools in characterization of this receptor system.


Biochem Pharmacol


Biochemical pharmacology


Ng DS,Isom GE




Has Abstract


1985-08-15 00:00:00














  • Rat CYP24A1 acts on 20-hydroxyvitamin D(3) producing hydroxylated products with increased biological activity.

    abstract::20-Hydroxyvitamin D(3) (20(OH)D(3)), the major product of CYP11A1 action on vitamin D(3), is biologically active and is produced in vivo. As well as potentially having important physiological actions, it is of interest as a therapeutic agent due to its lack of calcemic activity. In the current study we have examined t...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Tieu EW,Tang EK,Chen J,Li W,Nguyen MN,Janjetovic Z,Slominski A,Tuckey RC

    更新日期:2012-12-15 00:00:00

  • Allosteric regulation of cloned m1-m5 muscarinic receptor subtypes.

    abstract::Allosteric regulation of [3H]N-methylscopolamine [( 3H]NMS) and [3H]quinuclidinyl benzilate [( 3H]QNB) dissociation from the m1-m5 muscarinic receptor subtypes was examined in transfected CHO-K1 cells. Half-times of dissociation of [3H]NMS from cell membranes (at 23 degrees) ranged from less than 5 min for the m2 subt...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Ellis J,Huyler J,Brann MR

    更新日期:1991-10-24 00:00:00

  • Stereoselective metabolism of 3-isopropyl-5-(1-naphthoxymethyl)oxazolidine, a prodrug of propranolol.

    abstract::This investigation suggests that the oxazolidine derivative of propranolol is a prodrug which is hydrolysed stereoselectively to propranolol by hepatic post-mitochondrial supernatant. The (S)-form of the prodrug is more stable in the biological system than its (R)-form. ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Kamal A,Rao AB,Rao MV

    更新日期:1991-05-01 00:00:00

  • The possible involvement of the phospholipid phase of membranes in mediating the effects of verapamil on Ca2+ transport.

    abstract::The effect of verapamil in a model system of A23187-induced Ca2+-uptake into liposomes was studied. This was done in order to separate the effects of verapamil on the lipid phase of membranes from its effects on membraneous proteins. In the absence of A23187, the liposomes exhibited a very low Ca2+ permeability, which...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Erdreich A,Rahamimoff H

    更新日期:1987-06-01 00:00:00

  • Antimalarial activity of a 4',5'-unsaturated 5'-fluoroadenosine mechanism-based inhibitor of S-adenosyl-L-homocysteine hydrolase.

    abstract::A 4',5'-unsaturated 5'-fluoroadenosine inhibitor of S-adenosyl-L-homocysteine hydrolase (SAH hydrolase; EC, MDL 28842, was found to inhibit markedly the growth of Plasmodium falciparum in vitro and Plasmodium berghei in mice. Inhibition of P. berghei growth was associated with a large increase in the concentr...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Bitonti AJ,Baumann RJ,Jarvi ET,McCarthy JR,McCann PP

    更新日期:1990-08-01 00:00:00

  • Quilamine HQ1-44, an iron chelator vectorized toward tumor cells by the polyamine transport system, inhibits HCT116 tumor growth without adverse effect.

    abstract::Tumor cell growth requires large iron quantities and the deprivation of this metal induced by synthetic metal chelators is therefore an attractive method for limiting the cancer cell proliferation. The antiproliferative effect of the Quilamine HQ1-44, a new iron chelator vectorized toward tumor cells by a polyamine ch...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Renaud S,Corcé V,Cannie I,Ropert M,Lepage S,Loréal O,Deniaud D,Gaboriau F

    更新日期:2015-08-01 00:00:00

  • Effect of DNA conformation on cisplatin adduct formation.

    abstract::The anticancer drug cis-diamminedichloroplatinum(II) (cisplatin) has been shown previously to form adducts preferentially within internucleosomal or linker DNA rather than to DNA within the nucleosome. To determine whether other "open" regions of chromatin have an increased affinity for cisplatin, adduct formation wit...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Bubley GJ,Xu J,Kupiec N,Sanders D,Foss F,O'Brien M,Emi Y,Teicher BA,Patierno SR

    更新日期:1996-03-08 00:00:00

  • Multiple effects of staurosporine, a kinase inhibitor, on thymocyte functions. Comparison with the effect of tyrosine kinase inhibitors.

    abstract::The effects of staurosporine, a protein kinase inhibitor, on the signal transduction and proliferation of thymocytes were studied. Signal transduction in response to Concanavalin A (Con A) as well as Concanavalin A (Con A)-induced augmentation of [3H]inositol incorporation into phospholipids were inhibited by staurosp...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Zilberman Y,Gutman Y

    更新日期:1992-10-20 00:00:00

  • In vitro to in vivo extrapolation of the complex drug-drug interaction of bupropion and its metabolites with CYP2D6; simultaneous reversible inhibition and CYP2D6 downregulation.

    abstract::Bupropion is a widely used antidepressant and smoking cessation aid and a strong inhibitor of CYP2D6 in vivo. Bupropion is administered as a racemic mixture of R- and S-bupropion and has stereoselective pharmacokinetics. Four primary metabolites of bupropion, threo- and erythro-hydrobupropion and R,R- and S,S-OH-bupro...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Sager JE,Tripathy S,Price LS,Nath A,Chang J,Stephenson-Famy A,Isoherranen N

    更新日期:2017-01-01 00:00:00

  • A selective NaV1.1 activator with potential for treatment of Dravet syndrome epilepsy.

    abstract::Dravet syndrome (DS) is a catastrophic epileptic encephalopathy characterised by childhood-onset polymorphic seizures, multiple neuropsychiatric comorbidities, and increased risk of sudden death. Heterozygous loss-of-function mutations in one allele of SCN1A, the gene encoding the voltage-gated sodium channel 1.1 (NaV...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Chow CY,Chin YKY,Ma L,Undheim EAB,Herzig V,King GF

    更新日期:2020-11-01 00:00:00

  • Liposomal incorporation changes the effect of 1.25-dihydroxyvitamin D3 on the phospholipase C signal transduction pathway and the eicosanoid cascade on keratinocytes in vitro.

    abstract::1.25-dihydroxyvitamin D3 is of clinical importance (e.g. in the treatment of psoriasis) given its ability to regulate the proliferation and differentiation of human keratinocytes. 1.25-Dihydroxyvitamin D3 mediates its action via genomic and nongenomic pathways. The nongenomic actions begin with the activation of phosp...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Prüfer K,Jirikowski GF

    更新日期:1996-02-09 00:00:00

  • Fas/CD95-mediated apoptosis in human glioblastoma cells: a target for sensitisation to topoisomerase I inhibitors.

    abstract::The expression of the death receptor Fas/CD95 is cell type-specific and can be modulated by different cytotoxic treatments. In spite of a frequent expression of Fas/CD95 in high-grade gliomas, these tumours are typically refractory to conventional therapy. Using a human glioblastoma cell line (GBM), we explored the po...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Ciusani E,Perego P,Carenini N,Corna E,Facchinetti F,Boiardi A,Salmaggi A,Zunino F

    更新日期:2002-03-01 00:00:00

  • Properties of the mineralocorticoid receptor immunopurified from bovine kidney.

    abstract::The mineralocorticoid receptor (MCR) from bovine kidney was purified on an affinity column containing covalently linked polyclonal IgG raised in the rabbit against rat kidney protein purified in the presence of RU 26752 that is specific to the MCR. The immuno-affinity eluate was excluded as a single peak during gel pe...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Pagano M,Mirshahi M,Mirshahi F,Monnot M,Lazar G,Chassaing CG,Fermandjian S,Agarwal MK

    更新日期:1994-09-15 00:00:00

  • Phorbol 12-myristate 13-acetate (PMA)-induced migration of glioblastoma cells is mediated via p38MAPK/Hsp27 pathway.

    abstract::We investigated the mechanism of phorbol 12-myristate 13-acetate (PMA)-induced migration of glioblastoma cells focusing on the p38 mitogen-activated protein kinase (MAPK)/heat shock protein 27 (Hsp27) pathway. PMA-induced cell migration and activation of p38MAPK in A172 glioblastoma cells. PMA-induced formation of lam...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Nomura N,Nomura M,Sugiyama K,Hamada J

    更新日期:2007-09-01 00:00:00

  • Effects of CDP-choline on phospholipase A2 and cholinephosphotransferase activities following a cryogenic brain injury in the rabbit.

    abstract::Within the tissue surrounding the necrotic lesion, following a cryogenic injury of the brain, there is a definite activation of phospholipase A2 (at 2 and 4 hr post lesion) that accounts, at least in part, for the phospholipid breakdown. There is also an activation of cholinephosphotransferase (at 2 hr post lesion) th...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Arrigoni E,Averet N,Cohadon F

    更新日期:1987-11-01 00:00:00

  • Interactions of the anti-tumor ametantrone and mitoxantrone with rat hepatic microsomes.

    abstract::The interaction of the anti-tumor anthraquinones, ametantrone and mitoxantrone, with rat hepatic microsomes has been studied with a fluorescence technique using 7,12-dimethylbenzanthracene as a new fluorescent probe. The two drugs were able to quench the intrinsic fluorescence of microsomal suspension. Mitoxantrone wa...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: al-Gailany KA,Alwan AH

    更新日期:1988-10-15 00:00:00

  • Relationship between alpha-naphthylisothiocyanate-induced liver injury and elevations in hepatic non-protein sulfhydryl content.

    abstract::Acute administration of alpha-naphthylisothiocyanate (ANIT) to rats has been used as a model of intrahepatic cholestasis. The mechanism of toxicity of ANIT is unknown, although recent evidence suggests a causal or permissive role for glutathione (GSH) (Dahm LJ and Roth RA, Biochem Pharmacol 42: 1181-1188, 1991). In th...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Dahm LJ,Bailie MB,Roth RA

    更新日期:1991-08-22 00:00:00

  • Intestinal cell-specific vitamin D receptor (VDR)-mediated transcriptional regulation of CYP3A4 gene.

    abstract::CYP3A4 is the most important drug-metabolizing enzyme that is involved in biotransformation of more than 50% of drugs. Pregnane X receptor (PXR) dominantly controls CYP3A4 inducibility in the liver, whereas vitamin D receptor (VDR) transactivates CYP3A4 in the intestine by secondary bile acids. Four major functional P...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Pavek P,Pospechova K,Svecova L,Syrova Z,Stejskalova L,Blazkova J,Dvorak Z,Blahos J

    更新日期:2010-01-15 00:00:00

  • The stereospecific incorporation of fenoprofen into rat hepatocyte and adipocyte triacylglycerols.

    abstract::The formation of triacylglycerols containing fenoprofen was studied in rat isolated adipocytes and hepatocytes incubated with [3H]glycerol and R or S fenoprofen. In both hepatocytes and adipocytes there was a high-affinity enzymatic process for the synthesis of triacylglycerol containing fenoprofen which was stereospe...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Sallustio BC,Meffin PJ,Knights KM

    更新日期:1988-05-15 00:00:00

  • Rat liver kininase, a serine peptidase.

    abstract::A serine peptidase (RLK1) was partially purified from rat liver homogenates. Its molecular weight was 80,000, and its optimum pH was 7.5. Bz-Tyr-O-Et was hydrolyzed by the enzyme, which was inhibited by Ip2PF, PMSF and by Tos-Phe-CH2Cl. The bonds cleaved by the enzyme were Phe5-Ser6 and Phe8-Arg9, when bradykinin was ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Da Graça M,Mazzacoratti N,Sampaio CA

    更新日期:1982-03-01 00:00:00

  • Naringenin: a weakly estrogenic bioflavonoid that exhibits antiestrogenic activity.

    abstract::Treatment of immature 21-day-old female Sprague-Dawley rats with 17 beta-estradiol (E2) (0.5 microgram/rat) caused a significant increase in uterine wet weight, DNA synthesis, progesterone receptor (PR) binding, and peroxidase activity. At doses as high as 40 mg/rat, the bioflavonoid naringenin did not cause a signifi...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Ruh MF,Zacharewski T,Connor K,Howell J,Chen I,Safe S

    更新日期:1995-10-26 00:00:00

  • Contribution of phosphodiesterase isoenzymes and cyclic nucleotide efflux to the regulation of cyclic GMP levels in aortic smooth muscle cells.

    abstract::Involvement of phosphodiesterase isoenzymes (PDEs) in guanosine-3',5'-cyclic monophosphate (cGMP) hydrolysis was analyzed in aortic smooth muscle cells. Four families of PDEs were separated from pig aorta: PDE1 (calcium-calmodulin-activated), PDE3 (cGMP-inhibited), PDE4 (adenosine 3',5'-cyclic monophosphate [cAMP]-spe...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Mercapide J,Santiago E,Alberdi E,Martinez-Irujo JJ

    更新日期:1999-11-15 00:00:00

  • Heme oxygenase (HO)-1 induction prevents Endoplasmic Reticulum stress-mediated endothelial cell death and impaired angiogenic capacity.

    abstract::Most of diabetic cardiovascular complications are attributed to endothelial dysfunction and impaired angiogenesis. Endoplasmic Reticulum (ER) and oxidative stresses were shown to play a pivotal role in the development of endothelial dysfunction in diabetes. Hemeoxygenase-1 (HO-1) was shown to protect against oxidative...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Maamoun H,Zachariah M,McVey JH,Green FR,Agouni A

    更新日期:2017-03-01 00:00:00

  • α1A-Adrenoceptors activate mTOR signalling and glucose uptake in cardiomyocytes.

    abstract::The capacity of G protein-coupled receptors to modulate mechanistic target of rapamycin (mTOR) activity is a newly emerging paradigm with the potential to link cell surface receptors with cell survival. Cardiomyocyte viability is linked to signalling pathways involving Akt and mTOR, as well as increased glucose uptake...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Sato M,Evans BA,Sandström AL,Chia LY,Mukaida S,Thai BS,Nguyen A,Lim L,Tan CYR,Baltos JA,White PJ,May LT,Hutchinson DS,Summers RJ,Bengtsson T

    更新日期:2018-02-01 00:00:00

  • On the G protein-coupling selectivity of the native A2B adenosine receptor.

    abstract::A2B adenosine receptor (A2BAR) activation induces Gs-dependent cyclic AMP accumulation. However, A2BAR G protein-coupling to other signaling events, e.g. ERK1/2 and calcium, is not well documented. We explored Gi, Gq/11 and Gs coupling in 1321 N1 astrocytoma, HEK293, and T24 bladder cancer cells endogenously expressin...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Gao ZG,Inoue A,Jacobson KA

    更新日期:2018-05-01 00:00:00

  • Inhibition of MMPs and ADAM/ADAMTS.

    abstract::Matrix metalloproteinases (MMPs), A Disintegrin and Metalloproteinase (ADAM) and A Disintegrin and Metalloproteinase with Thrombospondin Motif (ADAMTS) are zinc-dependent endopeptidases that play a critical role in the destruction of extracellular matrix proteins and, the shedding of membrane-bound receptor molecules ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章,评审


    authors: Malemud CJ

    更新日期:2019-07-01 00:00:00

  • Inactivation of Wnt/β-catenin/renin angiotensin axis by tumor necrosis factor-alpha inhibitor, infliximab, ameliorates CKD induced in rats.

    abstract::Infliximab (IFX), a chimeric monoclonal antibody against tumor necrosis factor-α (TNF-α), is widely used to treat autoimmune diseases and chronic diseases associated with inflammation. TNF-α was reported to inhibit klotho, reactivate β-catenin and cause tubular cell injury in vitro. Whether the inhibition of TNF-α can...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Younis NN,Mohamed HE,Shaheen MA,Abdelghafour AM,Hammad SK

    更新日期:2021-01-19 00:00:00

  • Human ATP-Binding Cassette transporters ABCB1 and ABCG2 confer resistance to CUDC-101, a multi-acting inhibitor of histone deacetylase, epidermal growth factor receptor and human epidermal growth factor receptor 2.

    abstract::CUDC-101 is the first small-molecule inhibitor designed to simultaneously inhibit epidermal growth factor receptor (EGFR), human epidermal growth factor receptor 2 (HER2) and histone deacetylase (HDAC) in cancer cells. Recently, in its first in human phase I study, CUDC-101 showed promising single agent activity again...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Wu CP,Hsiao SH,Su CY,Luo SY,Li YQ,Huang YH,Hsieh CH,Huang CW

    更新日期:2014-12-15 00:00:00

  • Interaction of the EGFR inhibitors gefitinib, vandetanib, pelitinib and neratinib with the ABCG2 multidrug transporter: implications for the emergence and reversal of cancer drug resistance.

    abstract::Human ABCG2 is a plasma membrane glycoprotein that provides physiological protection against xenobiotics. ABCG2 also significantly influences biodistribution of drugs through pharmacological tissue barriers and confers multidrug resistance to cancer cells. Moreover, ABCG2 is the molecular determinant of the side popul...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Hegedüs C,Truta-Feles K,Antalffy G,Várady G,Német K,Ozvegy-Laczka C,Kéri G,Orfi L,Szakács G,Settleman J,Váradi A,Sarkadi B

    更新日期:2012-08-01 00:00:00

  • Effects of cationic diamidines on polyamine content and uptake on Leishmania infantum in in vitro cultures.

    abstract::The effect of a series of cationic diamidines recently synthesized by Ciba Geigy, bearing diarylic (CGP040215A and CGP039937A) or monoarylic moieties (CGP033829A, CGP035537A and CGP036958A), was analyzed on some metabolic targets and cell proliferation of in vitro cultures of Leishmania infantum promastigotes (insect ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Calonge M,Johnson R,Balaña-Fouce R,Ordóñez D

    更新日期:1996-09-27 00:00:00