Hepatic microsomal metabolism of the dichloroethanes.


:The binding of 1,1-dichloroethane (1,1-DCE) to the substrate binding site of hepatic microsomal cytochrome P-450, and the stimulation of hepatic microsomal CO-inhibitable NADPH oxidation by 1,1-DCE and 1,2-dichloroethane (1,2-DCE) were enhanced by induction with phenobarbital but not with beta-naphthoflavone. Incubation of the dichloroethanes with hepatic microsomes from phenobarbital-treated rats, NADPH-generating system and EDTA resulted in the conversion of 1,1-DCE to acetic acid and to a lesser extent to 2,2-dichloroethanol and probably also mono- and dichloroacetic acid and the conversion of 1,2-DCE to chloroacetaldehyde and to a lesser extent to chloroacetic acid and probably 2-chloroethanol. In addition, reaction mixtures constituted as described above resulted in slight but significant losses (ca. 13%) of hepatic microsomal cytochrome P-450. The omission of dichloroethane or the NADPH-generating system from incubation mixtures eliminated the above effects, and SKF-525A or CO diminished or eliminated the effects. Pathways for the metabolism of 1,1-DCE and 1,2-DCE are proposed.


Biochem Pharmacol


Biochemical pharmacology


McCall SN,Jurgens P,Ivanetich KM




Has Abstract


1983-01-15 00:00:00














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    abstract::Naturally occurring amino acid substitutions conferring resistance to methotrexate (MTX) have been reported previously at codon positions 22 (leu-->arg, phe) and 31 (phe-->ser, trp) of mammalian dihydrofolate reductases (DHFR). To explore the character of other substitutions, a polymerase chain reaction (PCR)-assisted...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Morris JA,McIvor RS

    更新日期:1994-03-29 00:00:00

  • Inhibition of phosphate transport in rat heart mitochondria by 3'-azido-3'-deoxythymidine due to stimulation of superoxide anion mitochondrial production.

    abstract::In order to gain some insight into the mechanism by which 3'-azido-3'-deoxythymidine (AZT) damages mitochondria, we investigated whether externally added AZT can stimulate reactive oxygen species (ROS) production by rat heart mitochondria (RHM). An increase in superoxide anion ((O(2)(.-)) production was measured in RH...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Valenti D,Atlante A,Barile M,Passarella S

    更新日期:2002-07-15 00:00:00

  • Positive allosteric modulators as an approach to nicotinic acetylcholine receptor-targeted therapeutics: advantages and limitations.

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章,评审


    authors: Williams DK,Wang J,Papke RL

    更新日期:2011-10-15 00:00:00

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Teschke R,Wannagat FJ,Löwendorf F,Strohmeyer G

    更新日期:1986-02-01 00:00:00

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    journal_title:Biochemical pharmacology

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    更新日期:2020-05-01 00:00:00

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Bitonti AJ,Baumann RJ,Jarvi ET,McCarthy JR,McCann PP

    更新日期:1990-08-01 00:00:00

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Rode W,Kulikowski T,Kedzierska B,Jastreboff M,Shugar D

    更新日期:1984-09-01 00:00:00

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章,评审


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    更新日期:2020-02-01 00:00:00

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Ko JC,Zheng HY,Chen WC,Peng YS,Wu CH,Wei CL,Chen JC,Lin YW

    更新日期:2016-12-15 00:00:00

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


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    更新日期:1998-12-01 00:00:00

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    authors: De Clerck F,Van Nueten JM

    更新日期:1983-03-01 00:00:00

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    pub_type: 杂志文章


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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


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    更新日期:1989-09-15 00:00:00

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


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    更新日期:1985-06-01 00:00:00

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Rouach H,Houze P,Gentil M,Orfanelli MT,Nordmann R

    更新日期:1994-05-18 00:00:00

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Heubel F,Reuter T,Gerstner E

    更新日期:1989-04-15 00:00:00

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Raza H,Otaiba A,Montague W

    更新日期:1995-10-26 00:00:00

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Mimouni M,Bontemps F,Van den Berghe G

    更新日期:1995-11-09 00:00:00

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    pub_type: 杂志文章


    authors: Day SA,Lakner AM,Moore CC,Yen MH,Clemens MG,Wu ES,Schrum LW

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    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Howard AJ,Mitchell CE,Dutcher JS,Henderson TR,McClellan RO

    更新日期:1986-07-01 00:00:00