Abstract：
:Engelbreth-Holm-Swarm (EHS) tumor cells were utilized as a model for investigating the production of basement membrane components. These cells contain two immunologically distinct NADPH-dependent reductases, aldose reductase (EC 1.1.1.21) and aldehyde reductase (EC 1.1.1.2), which were purified to apparent homogeneity by a combination of procedures which included ammonium sulfate fractionation, Sephadex G-75 gel filtration, Matrex Gel Orange A affinity chromatography, and chromatofocusing on Pharmacia Mono P. The molecular weights of aldose and aldehyde reductases were estimated to be 38K and 40K, respectively, by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Substrate specificity studies showed that both enzymes were capable of reducing a variety of aldehydes to their respective alcohols; however, only aldehyde reductase oxidized L-gulonic acid. Surprisingly, both enzymes showed similar reactivities with D-glucose and D-galactose, suggesting that both aldose and aldehyde reductases may contribute to sorbitol production in the EHS tumor cell. The activities of both enzymes were increased by the presence of sulfate ion, but chloride ion decreased the activity of aldose reductase. Both aldose and aldehyde reductases were inhibited by a series of structurally diverse aldose reductase inhibitors.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Tanimoto T,Sato S,Kador PFdoi
10.1016/0006-2952(90)90049-qsubject
Has Abstractpub_date
1990-02-01 00:00:00pages
445-53issue
3eissn
0006-2952issn
1873-2968pii
0006-2952(90)90049-Qjournal_volume
39pub_type
杂志文章abstract：:The transforming growth factor-beta (TGF-beta) family of regulatory growth factors can reversibly arrest cell division in the G1 phase of the cell cycle. Previously, TGF-beta3 was shown to protect epithelial cells and hematopoietic cells from cytotoxic damage in vitro and in vivo, and to reduce the severity and durati...
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pub_type： 杂志文章
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更新日期：2004-02-15 00:00:00
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更新日期：2011-04-15 00:00:00
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更新日期：2009-09-15 00:00:00
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pub_type： 杂志文章
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更新日期：1995-11-09 00:00:00
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更新日期：2013-07-01 00:00:00
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更新日期：1991-04-15 00:00:00
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journal_title：Biochemical pharmacology
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doi：10.1016/0006-2952(95)00084-d
更新日期：1995-07-17 00:00:00
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pub_type： 杂志文章
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更新日期：2018-09-01 00:00:00
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更新日期：2020-10-01 00:00:00
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更新日期：2003-10-01 00:00:00
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更新日期：1994-02-11 00:00:00
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更新日期：1989-06-01 00:00:00
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更新日期：1984-07-15 00:00:00
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pub_type： 杂志文章
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更新日期：2009-03-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：2020-02-01 00:00:00
