Abstract:
:When the human cell line NHIK 3025 was exposed to retinoic acid (1 nM; 10 microM), the cell cycle time was prolonged. Experiments using cells synchronized by mitotic selection showed that the retinoic acid induced growth delay was barely seen within the first cell cycle after exposure to 10 microM retinoic acid, whereas the next cell cycle durations were increased 30-60%. The effect was reversible as normal growth rate was restored after removal of the drug. DNA histograms indicated a prolongation of G1 of the cell cycle. We have shown earlier that glucocorticoid steroids also induce a prolongation of the cell cycle, located within G1. When the cells were exposed to the synthetic glucocorticoid, dexamethasone, in addition to retinoic acid, no additive effect was found; on the contrary, growth inhibition was less than that with retinoic acid alone. Dexamethasone from 1 nM upwards antagonized the growth inhibitory effect of retinoic acid. This glucocorticoid mediated effect seemed to be mediated via the glucocorticoid receptor, as no effect was seen when the receptor was blocked by the antagonist 17 beta hydroxy-11 beta, 4-dimethylaminophenyl-17 alpha-propynyl estra 4,9 diene-3-one [RU 38486]. The growth inhibition studies were supported by morphological observations showing that dexamethasone induced cytoskeletal alterations dominated when the cells were exposed to both drugs simultaneously. These findings might be of importance in cancer therapy where both drugs are used.
journal_name
Cancer Resjournal_title
Cancer researchauthors
Bakke Osubject
Has Abstractpub_date
1986-03-01 00:00:00pages
1275-9issue
3eissn
0008-5472issn
1538-7445journal_volume
46pub_type
杂志文章相关文献
CANCER RESEARCH文献大全abstract::In the present study, we examined the presence of deacetylases capable of producing free hexosamines, which we have shown earlier to be immunosuppressive against human natural killer (NK) cell-mediated cytotoxicity, from N-acetylhexosamines in human tumor cells. When human NK-resistant colon cancer cells (Colo-320DM) ...
journal_title:Cancer research
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
abstract::Rous sarcoma virus-transformed hamster BHK fibroblasts express a virus-induced cell surface antigen undetectable in cells either transformed by unrelated viruses or infected by transformation-defective strains of Rous sarcoma virus. To clarify whether this antigen plays any role in the process of malignant transformat...
journal_title:Cancer research
pub_type: 杂志文章
doi:
更新日期:1979-11-01 00:00:00
abstract::Low-dose cyclophosphamide (LDC) induces selective apoptosis of endothelial cells within the vascular bed of tumors. Here, we investigated a hypothesis that the effect of LDC is mediated by the pro-apoptotic action of endogenous inhibitors of angiogenesis. Tumors treated with LDC demonstrate similar expression of matri...
journal_title:Cancer research
pub_type: 杂志文章
doi:10.1158/0008-5472.can-03-3126
更新日期:2004-03-01 00:00:00
abstract::Combination of chemotherapy and immunotherapy to increase the effectiveness of an antitumor immune response is currently regarded as an attractive antitumor strategy. In a pilot clinical trial, we have recently documented an increase of melanoma antigen A (Melan-A)-specific, tumor-reactive, long-lasting effector-memor...
journal_title:Cancer research
pub_type: 杂志文章
doi:10.1158/0008-5472.CAN-10-1326
更新日期:2010-09-15 00:00:00
abstract::Possible mechanisms whereby alcohol abuse and alcohol-related diseases may promote the development of cancer are analyzed. The mechanisms discussed include: (a) contact-related local effects on the upper gastrointestinal tract; (b) the presence of low levels of carcinogens in alcoholic beverages; (c) induction of micr...
journal_title:Cancer research
pub_type: 杂志文章,评审
doi:
更新日期:1979-07-01 00:00:00
abstract::Sarcoma growth factor (SGF) induces proliferation and anchorage-independent growth of nonmalignant cells. It competes with epidermal growth factor (EGF) for the EGF-receptors at the cell surface. SGF-like factors have recently been isolated from embryos, suggesting that SGFs may represent embryonic forms of EGF. There...
journal_title:Cancer research
pub_type: 杂志文章
doi:
更新日期:1983-12-01 00:00:00
abstract::Activated leukocyte cell adhesion molecule (ALCAM/CD166/MEMD) could function as a cell surface sensor for cell density, controlling the transition between local cell proliferation and tissue invasion in melanoma progression. We have tested the hypothesis that progressive cell clustering controls the proteolytic cascad...
journal_title:Cancer research
pub_type: 杂志文章
doi:10.1158/0008-5472.CAN-05-0378
更新日期:2005-10-01 00:00:00
abstract::Cigarette smoking causes cancer and DNA mutations. However, long-term chronic exposure to smoke is believed to be necessary for carcinogenesis. Here, we investigate the relationship between short-term exposure to smoke and the frequency of deletions in the mouse embryo. Deletions and other genome rearrangements are as...
journal_title:Cancer research
pub_type: 杂志文章
doi:
更新日期:1998-06-15 00:00:00
abstract::Tn and sialylated Tn (sTn) are blood group-related epitopes expressed on mucins of colon carcinoma and other epithelial tumors and are, therefore, potential targets for immunological control. We have immunized 20 colorectal cancer patients at high risk for recurrence with a vaccine consisting of partially desialylated...
journal_title:Cancer research
pub_type: 杂志文章
doi:
更新日期:1992-10-15 00:00:00
abstract::The Chinese hamster ovary cell line xrs-1 is hypersensitive to gamma-radiation. This sensitivity has been attributed to an inability of this cell line to efficiently repair gamma-ray induced double-strand breaks (DSBs). We have recently reported that xrs-1 is also sensitive to topoisomerase II inhibitors that stabiliz...
journal_title:Cancer research
pub_type: 杂志文章
doi:
更新日期:1990-09-15 00:00:00
abstract::The expression of cytochrome P450 genes directly within target cells is an important determinant of human susceptibility to cancers, birth defects, and other chemically initiated diseases. One pivotal gene, CYP1A1, codes for an inducible cytochrome P450 isozyme (P450IA1) responsible for the bioactivation of numerous c...
journal_title:Cancer research
pub_type: 杂志文章
doi:
更新日期:1990-07-15 00:00:00
abstract::The correlation between telomerase activity, telomere lengths, and cellular replicative capacity has led to the theory that maintenance of telomere lengths by telomerase acts as a molecular clock to control replicative capacity and senescence. Regulation of this molecular clock may have applications in the treatment o...
journal_title:Cancer research
pub_type: 杂志文章
doi:
更新日期:1998-09-15 00:00:00
abstract::CAMA-1 cells, isolated from malignant pleural effusion, are grown in long-term cultures as monolayers. The rate of growth is dependent upon fetal bovine serum and estradiol. These cells also exhibit a dose response to added 17 beta-estradiol with respect to the incorporation of radiolabeled thymidine into cells. The u...
journal_title:Cancer research
pub_type: 杂志文章
doi:
更新日期:1982-12-01 00:00:00
abstract::Bleomycin treatment produced dose-dependent changes in lung collagen content and in several measurable histopathological parameters. NIH/Swiss mice were treated twice weekly for 6 weeks with bleomycin, 0, 1, 20, or 40 mg/kg s.c. The two highest doses produced mortalities of 35 and 100%, respectively, as well as loss o...
journal_title:Cancer research
pub_type: 杂志文章
doi:
更新日期:1978-03-01 00:00:00
abstract::Normal bronchial epithelial cells (NBECs) are at risk for damage from inhaled and endogenous oxidative species and from epoxide metabolites of inhaled polycyclic aromatic hydrocarbons. Epidemiological and in vitro data suggest that interindividual variation in this risk may result from variation in NBEC expression of ...
journal_title:Cancer research
pub_type: 杂志文章
doi:
更新日期:2000-03-15 00:00:00
abstract::The renal uptake of radiolabeled antibody fragments and peptides is a problem in radioimmunodetection and radioimmunotherapy, especially with intracellular retained radiometals. The aim of this study was to develop suitable methods to reduce this kidney uptake. BALB/c mice or nude mice bearing the human GW-39 colon ca...
journal_title:Cancer research
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
abstract::The discovery of somatic mutations in EGFR and development of EGFR tyrosine kinase inhibitors (TKI) have revolutionized treatment for lung cancer. However, resistance to TKIs emerges in almost all patients and currently no effective treatment is available. Here, we show that β-catenin is essential for development of E...
journal_title:Cancer research
pub_type: 杂志文章
doi:10.1158/0008-5472.CAN-14-0184
更新日期:2014-10-15 00:00:00
abstract::Neocarzinostatin (NCS) is mutagenic in bacteria, yeast, fungi, and mammalian cells. In cell-free systems, DNA strand breakage induced by NCS requires a reducing agent like 2-mercaptoethanol, unless very high (greater than 100 micrograms/ml) concentrations of NCS are used. In this study, we have investigated the role o...
journal_title:Cancer research
pub_type: 杂志文章
doi:
更新日期:1985-10-01 00:00:00
abstract::Incidence of the loss of heterozygosity on chromosome 3p was evaluated using 7 polymorphic probes in 35 Japanese patients with sporadic renal cell carcinoma (RCC). Overall frequency of the loss of heterozygosity on 3p was 53%, representing 16 of 30 informative cases. Examination of the relationship between histopathol...
journal_title:Cancer research
pub_type: 杂志文章
doi:
更新日期:1991-02-01 00:00:00
abstract::The potential role of alpha5beta1 integrins in cancer has recently attracted much interest. However, few alpha5beta1-selective antagonists have been developed compared with other integrins. The most specific nonpeptidic alpha5beta1 antagonist described thus far, SJ749, inhibits angiogenesis by affecting adhesion and m...
journal_title:Cancer research
pub_type: 杂志文章
doi:10.1158/0008-5472.CAN-05-4105
更新日期:2006-06-15 00:00:00
abstract::Overexpression of ABCB1 (MDR1) P-glycoprotein, a multidrug efflux pump, is one mechanism by which tumor cells may develop multidrug resistance (MDR), preventing the successful chemotherapeutic treatment of cancer. Sesquiterpenes from Celastraceae family are natural compounds shown previously to reverse MDR in several ...
journal_title:Cancer research
pub_type: 杂志文章
doi:10.1158/0008-5472.CAN-04-1005
更新日期:2004-10-01 00:00:00
abstract::A new form of therapy of experimental tumors, utilizing lymphokine-activated killer (LAK) cells and high doses of interleukin 2, has recently been applied in the treatment of human neoplasms. Severe side effects, suggestive of a diffuse vascular injury of unknown etiology, have prevented a more widespread application ...
journal_title:Cancer research
pub_type: 杂志文章
doi:
更新日期:1988-10-01 00:00:00
abstract::Ornithine decarboxylase (ODC) is a rate-limiting enzyme in the first step of polyamine biosynthesis that is associated with cell growth and tumor formation. Existing catalytic inhibitors of ODC have lacked efficacy in clinical testing or displayed unacceptable toxicity. In this study, we report the identification of a...
journal_title:Cancer research
pub_type: 杂志文章
doi:10.1158/0008-5472.CAN-15-0442
更新日期:2016-03-01 00:00:00
abstract::Melanoma incidence is increasing worldwide, and metastatic melanoma is almost completely resistant to every known therapy. New approaches to treating melanoma are urgently needed, and a greater understanding of the biology of melanoma invasion and metastasis will aid in their creation. A high proportion of invasive me...
journal_title:Cancer research
pub_type: 杂志文章
doi:10.1158/0008-5472.CAN-07-1791
更新日期:2007-11-15 00:00:00
abstract::The immune system is a robust and often untapped accomplice of many standard cancer therapies. A majority of tumors exist in a state of immune tolerance where the patient's immune system has become insensitive to the cancer cells. Because of its lymphodepleting effects, chemotherapy has the potential to break this tol...
journal_title:Cancer research
pub_type: 杂志文章
doi:10.1158/0008-5472.CAN-18-3712
更新日期:2019-10-15 00:00:00
abstract::A simple method is described for quantitating pulmonary metastases in C57BL/6J mice implanted with the Lewis T241 fibrosarcoma. Lungs were removed from tumor-bearing mice, homogenized, and diluted to 14 ml with supplemented Roswell Park Memorial Institute Tissue Culture Medium 1640 (+5% fetal calf serum). Twenty-five ...
journal_title:Cancer research
pub_type: 杂志文章
doi:
更新日期:1983-12-01 00:00:00
abstract::Although more than 100 different BRCA1 germ-line mutations have already been identified in breast and/or ovarian cancer families, we report for the first time a deleterious genomic rearrangement in BRCA1. A 1-kb deletion comprising exon 17 was found in a large breast and ovarian cancer family, leading to a frameshift ...
journal_title:Cancer research
pub_type: 杂志文章
doi:
更新日期:1997-03-01 00:00:00
abstract::15-Lipoxygenase (15-LOX)-2 is expressed in benign prostate secretory cells and benign prostate produces 15S-hydroxyeicosatetraenoic acid (15S-HETE) from exogenous arachidonic acid (AA). In contrast, 15S-LOX-2 and 15S-HETE formation are reduced in prostate carcinoma (Pca). The mechanisms whereby reduced 15-LOX-2 may co...
journal_title:Cancer research
pub_type: 杂志文章
doi:
更新日期:2001-01-15 00:00:00
abstract::The conversion of transforming growth factor beta (TGF-beta) from a tumor suppressor to a tumor promoter occurs frequently during mammary tumorigenesis, yet the molecular mechanisms underlying this phenomenon remain undefined. We show herein that TGF-beta repressed nuclear factor-kappaB (NF-kappaB) activity in normal ...
journal_title:Cancer research
pub_type: 杂志文章
doi:10.1158/0008-5472.CAN-07-3094
更新日期:2008-03-01 00:00:00
abstract::The role of angiogenesis inhibition in the antitumor activity of recombinant murine interleukin 12 (rmIL-12) was studied in K1735 murine melanomas, the growth of which is rapidly and markedly suppressed by rmIL-12 treatment. On the basis of the prediction that tumor ischemia should result from therapeutic angiogenesis...
journal_title:Cancer research
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00