Abstract：
:Methods for improving the estrogen receptor (ER) and progestin receptor (PgR) assay were studied. Using DCC procedure as the basis, the extraction and incubation of ER and PgR is more efficiently achieved by phosphate buffer as compared with water, tris and veronal buffers. The multiple extractions of hormone receptors by any solvent system used is more complete than a single operation of homogenization and centrifugation. The utilization of combined extraction first with water and then phosphate buffer, followed by incubation of the cytosol in phosphate buffer with hormone is also an efficient procedure for the assay of both ER and PgR.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Chen YM,Rice RG,Vaughn CBsubject
Has Abstractpub_date
1986-07-01 00:00:00pages
589-94issue
4eissn
0250-7005issn
1791-7530journal_volume
6pub_type
杂志文章abstract：BACKGROUND:The altered expression of multidrug resistance 1 (MDR1) contributes to various types of carcinogenesis and their disease progression. Hypermethylation of CpG islands located in the promoter regions is involved in gene silencing at the transcriptional level. The methylation status of MDR1 gene promoter was in...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：:The proliferative rate as well as the activity of 3-hydroxy-3-methylglutaryl CoA (HMG CoA) reductase, which regulates de novo synthesis of mevalonate, was comparable in the two human breast cancer cell lines Hs578T and MDA-231 when cultured in the presence of serum. Upon treatment with mevinolin (an HMG CoA reductase ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
abstract：PURPOSE:Investigation of the oxygenation status of organotypic multicellular spheroids (OMS) and their response to irradiation. MATERIALS AND METHODS:Tumour specimens of glioblastoma multiforme patients (n = 16) were initiated as OMS. Following 20 Gy gamma-irradiation, the cell migratory capacity was evaluated. Sphero...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-03-01 00:00:00
abstract：:The Feulgen-DNA content of squamous carcinoma cell nuclei from the human esophagus was assessed in punch biopsies from 47 untreated patients. Forty-four of the 47 biopsies (93.6%) demonstrated aneuploid cell populations, and the remaining 3 (6.4%) were non-diploid. Previous studies have demonstrated that in esophageal...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1989-05-01 00:00:00
abstract：BACKGROUND:Plasmablastic lymphoma (PBL) is a rare entity which is often causally related to infection by the Human Immunodeficiency Virus (HIV). Despite its predilection for oral cavity involvement, multiple cases of extra-oral involvement have been reported in the literature, more often among immunocompetent individua...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-09-01 00:00:00
abstract：:K18 is a conjugate of human immunoglobulin G and melphalan and has been reported to accumulate selectively in the tumor. In the present study, the in vitro antitumor effect of K18 was assessed in a total of 107 fresh human tumors by DNA synthesis (3H-thymidine incorporation) inhibition assay, and compared with that of...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1989-05-01 00:00:00
abstract：AIM:The aim of the present study was to investigate the impact of cyclin E expression on cancer-specific survival, as well as on conventional clinocopathological and prognostic factors in endometrial endometrioid adenocarcinoma. MATERIALS AND METHODS:The study consisted of 211 patients surgically treated for endometri...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-06-01 00:00:00
abstract：BACKGROUND/AIM:The main objective of the present study was to evaluate the significance of apoptosis in the Fletcher's risk classification for gastrointestinal stromal tumors (GISTs). MATERIALS AND METHODS:Apoptotic cells were identified by immunostaining for single-stranded DNA (ssDNA). We assigned each GIST to one o...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12542
更新日期：2018-05-01 00:00:00
abstract：BACKGROUND:Although significant accumulation of prostaglandin E(2) (PGE(2)) in the human prostate cancer tissues has been reported, there is lack of substantial evidence regarding the key role of PGE(2)-induced E-prostanoid-4 receptor (EP4) on Snail, a master regulator of epithelial mesenchymal transition (EMT). In thi...
journal_title：Anticancer research
pub_type： 杂志文章,收录出版
doi：
更新日期：2011-12-01 00:00:00
abstract：BACKGROUND/AIM:Bevacizumab (BV) has been used for the treatment of recurrent glioblastoma. However, it also induces epithelial-mesenchymal transition (EMT) in glioblastoma cells, which compromises its efficacy. BATF2 (basic leucine zipper ATF-like transcription factor 2), a multi-target transcriptional repressor, has b...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11821
更新日期：2017-08-01 00:00:00
abstract：BACKGROUND:In our previous study, it was suggested that nitrotyrosine, a product of nitrogen species, found in esophageal squamous cell carcinoma (ESCC), may contribute to the progression of esophageal cancer. MATERIALS AND METHODS:To clarify whether nitrotyrosine expression is associated with apoptosis and/or angioge...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：:Human papillomaviruses (HPV) and their role in carcinogenesis have been the subject of extensive investigation Specific types of HPV have been associated with cervical carcinoma HPV 16 and 18 are mainly associated with malignant progression and considered "high risk" viruses Using Southern blot analysis and in situ hy...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:The intratumor and intramuscle distribution of blood flow and plasma volume was studied via isotope techniques in a transplantable rat fibrosarcoma. Administration of two prostaglandin synthesis inhibitors, indomethacin and diclofenac-sodium, had different effects. Indomethacin changed muscle plasma volume significant...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1984-05-01 00:00:00
abstract：BACKGROUND/AIM:The combination of bevacizumab (BEV) plus interferon alpha-2a (IFN) constitutes an option for first-line treatment of metastatic renal cell carcinoma. Real-world data from routine clinical practice are rare and were, therefore, collected during this non-interventional study (NIS). PATIENTS AND METHODS:A...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13188
更新日期：2019-02-01 00:00:00
abstract：BACKGROUND:Chkl is a checkpoint gene that is activated after DNA damage. It phosphorylates and inactivates Cdc25C at the late G2 phase. The inactivation of Cdc25C and consequently, the inactivation of Cdc2, are required for the G2 arrest induced by DNA damage. METHODS:We treated 184B5 cell line and its E6 transformed ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：BACKGROUND AND AIM:The clinical efficiency of cisplatin (CDDP) against gastric cancer is often limited by the development of resistance. A third-generation platinum-containing agent, oxaliplatin (L-OHP), has been introduced for treating gastric cancer. Here, we studied oxaliplatin in vitro to reveal the mechanism of ac...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-07-01 00:00:00
abstract：BACKGROUND:The area under the curve (AUC) of free carboplatin, is a major determinant of toxicity and response. A conventional pharmacokinetic study to determine AUC requires frequent blood sampling. One strategy for overcoming this problem is to apply a limited sampling model (LSM). MATERIALS AND METHODS:We developed...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：BACKGROUND:Since tumor markers can be increased in the course of many benign diseases, the aim of this study was to verify if this also occurs in the course of rheumatic diseases. MATERIALS AND METHODS:We investigated the incidence and characteristics of Tumor M2-PK in the EDTA-plasma of 137 patients suffering from rh...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-03-01 00:00:00
abstract：:Most current anticancer drugs have shown excellent therapeutic effects on human oral squamous cell carcinoma (OSCC), but they also produce potent cytotoxicity in normal oral keratinocytes. This review article summarizes our extensive research of tumor specificity and keratinocyte toxicity of nine groups of compounds s...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：10.21873/anticanres.12038
更新日期：2017-11-01 00:00:00
abstract：:In a prospective study comprising 447 women with endometrial carcinoma stages I-II, the prognostic significance of clinical and flow cytometric variables was evaluated in univariate and multivariate analyses. The parameters studied included age, uterine cavity depth, clinical stage, histopathologic grade, myometrial i...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1992-05-01 00:00:00
abstract：BACKGROUND:Apolipoprotein E (APOE), a lipid transport protein that has a key role in the lipoprotein metabolism, is expressed by macrophages under the control of the transcription factor Liver X Receptor (LXR), an oxysterol-activated transcriptional factor involved in cholesterol metabolism. Recent work has shown that ...
journal_title：Anticancer research
pub_type： 历史文章,杂志文章
doi：
更新日期：2013-09-01 00:00:00
abstract：BACKGROUND/AIM:DNA methylation is a common epigenetic change in cancer. However, microRNA (miRNA) regulation by epigenetic alteration, especially CpG island hypermethylation, remains poorly understood in esophageal squamous cell carcinoma (ESCC). MATERIALS AND METHODS:miRNAs which were up-regulated after de-methylatio...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-09-01 00:00:00
abstract：:The interleukins (IL)-6 and -10 and heat shock proteins (HSP) have an important role in the host-tumor interaction and in tumor bulk. HSP-90 may have a regulatory role in cytokine biosynthesis and prognostic value in some tumors. To define the role of IL-6, IL-10 and HSP-90 in prostate cancer progression the immunohis...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-09-01 00:00:00
abstract：BACKGROUND:Despite improvements in chemotherapy and surgery in the treatment of osteosarcoma, satisfactory results are still difficult to achieve. New therapeutic modalities need to be developed for the improvement of these treatments. TRAIL (TNF-related apoptosis inducing ligand) is known as a selective apoptosis indu...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-03-01 00:00:00
abstract：:We have developed a sensitive enzyme-linked immunosorbent assay (ELISA) procedure that uses a commercially-available anti-BrdUrd antibody for the quantitation of BrdUrd substituted into DNA. In our assay, 50% displacement occurs at 0.89 nM of BrdUrd in 2.2% BrdUrd-substituted DNA, which is equivalent to 2.47 ng of Brd...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1988-05-01 00:00:00
abstract：:A new transitional cell carcinoma cell line, BCCA-1, derived from a primary urinary bladder carcinoma, was characterized with respect to the growth patterns of in vitro culture, xenotransplantability in SCID mice and immunophenotypic profile. The most unusual finding was a strong tendency of forming many aggregates (m...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：:2-Amino-3-methylimidazo[4,5-f]quinoline (IQ) is a food mutagen which has been found to be carcinogenic in the Fischer-344 rat. We have assessed the effects of a diet containing menhaden oil on IQ-DNA adduct formation in several organs of the male Fischer-344 rat. Young adult, male Fischer-344 rats were maintained on e...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
abstract：:In the present work, we studied the expression of the c-myc oncoprotein p-62 and the ras oncoprotein p-21 in the dermal cellular infiltrate of paraffin embedded skin specimens, obtained from patients suffering from Mycosis Fungoides and Sezary syndrome. Nineteen specimens from early stage Mycosis Fungoides, nineteen f...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-07-01 00:00:00
abstract：:The purpose of these experiments was to test whether a brief course of insulin-like growth factor-1 (IGF-1) injection (escalating doses) concomitant to irradiation ameliorates radiation-induced kidney dysfunction and lethal bowel toxicity in a mouse model of unilateral high-dose irradiation of the kidney and adjacent ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:The circadian rhythm-modulated delivery of anticancer drugs has been shown to reduce toxicity and improve anticancer efficacy. The aim of this phase I trial was to compare the feasibility and tolerability of carboplatin (CBDCA) administered at circadian-modulated or flat infusion rate in 24 patients with advanced canc...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1996-05-01 00:00:00
