Effect of pH on fecal recovery of energy derived from volatile fatty acids.

Abstract:

:We assessed the effect of pH on volatilization of short-chain fatty acids during lyophilization. Acetic, propionic, valeric, and butyric acids were added to a fecal homogenate in amounts sufficient to raise the energy density by 18-27%. Fecal homogenate samples were either acidified (pH 2.8-3.2), alkalinized (pH 7.9-8.7), or left unchanged (4.0-4.8) prior to lyophilization and subsequent bomb calorimetry. Alkalinizing the fecal samples prevented the 20% loss of energy derived from each of these volatile fatty acids observed in samples either acidified or without pH adjustment. These data suggest that in energy balance studies involving subjects with active colonic fermentation, fecal samples should be alkalinized prior to lyophilization and bomb calorimetry.

authors

Kien CL,Liechty EA

subject

Has Abstract

pub_date

1987-11-01 00:00:00

pages

123-5

issue

6

eissn

0277-2116

issn

1536-4801

journal_volume

6

pub_type

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