Fission and refusion of red blood cells using a micropipette technique.

Abstract:

:In micropipette experiments with small capillaries and moderate high pressure difference (approximately 1000 Pa) cell fragmentation (fission) of human red blood cells without hemolysis was observed by TV-system for a large number of fresh red blood cells of different donors. After separation, the fragment moves away from the residual cell. In seven cases this process was evaluated quantitatively and was shown that the rate of the fragment was constant in time. Two mechanisms for this phenomenon are discussed. In particular cases a spontaneous re-fusion with the residual cell body in the capillary can be observed. In our opinion probably protein-depleted membrane surfaces arise and membrane fusion is possible simply by mechanical contact without additional electric fields and/or fusion agents.

journal_name

Biorheology

journal_title

Biorheology

authors

Meier W,Lerche D,Wendt MO,Paulitschke M

doi

10.3233/bir-1987-24302

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

287-96

issue

3

eissn

0006-355X

issn

1878-5034

journal_volume

24

pub_type

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