Quantification of subchondral bone changes in a murine osteoarthritis model using micro-CT.

Abstract:

:In the past few years there has been a considerable interest in the role of bone in osteoarthritis. Despite the increasing evidence of the involvement of bone in osteoarthritis, it remains very difficult to attribute the cause or effect of changes in subchondral bone to the process of osteoarthritis. Although osteoarthritis in mice provides a useful model to study changes in the subchondral bone, detailed quantification of these changes is lacking. Therefore, the goal of this study was to quantify subchondral bone changes in a murine osteoarthritis model by use of micro-computed tomography (micro-CT). We induced osteoarthritis-like characteristics in the knee joints of mice using collagenase injections, and after four weeks we calculated various 3D morphometric parameters in the epiphysis of the proximal tibia. The collagenase injections caused cartilage damage, visible in histological sections, particularly on the medial tibial plateau. Micro-CT analysis revealed that the thickness of the subchondral bone plate was decreased both at the lateral and the medial side. The trabecular compartment demonstrated a small but significant reduction in bone volume fraction compared to the contralateral control joints. Trabeculae in the collagenase-injected joints were thinner but their shape remained rod-like. Furthermore, the connectivity between trabeculae was reduced and the trabecular spacing was increased. In conclusion, four weeks after induction of osteoarthritis in the murine knee subtle but significant changes in subchondral bone architecture could be detected and quantified in 3D with micro-CT analysis.

journal_name

Biorheology

journal_title

Biorheology

authors

Botter SM,van Osch GJ,Waarsing JH,Day JS,Verhaar JA,Pols HA,van Leeuwen JP,Weinans H

subject

Has Abstract

pub_date

2006-01-01 00:00:00

pages

379-88

issue

3,4

eissn

0006-355X

issn

1878-5034

journal_volume

43

pub_type

杂志文章
  • Hemorheological effects of secoisolariciresinol in ovariectomized rats.

    abstract:BACKGROUND:Postmenopausal women often develop hemorheological disorders which may affect the systemic blood circulation and present a cardiovascular risk factor. OBJECTIVE:We evaluated effects of secoisolariciresinol (SECO), a phytoestrogen, on hemorheological parameters and lipid peroxidation in a model of the age-re...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-15066

    authors: Maslov MY,Plotnikova TM,Anishchenko AM,Aliev OI,Nifantiev NE,Plotnikov MB

    更新日期:2016-01-07 00:00:00

  • Required test duration for group comparisons in ligament viscoelasticity: a statistical approach.

    abstract::The goal of this study was to determine the duration of time that ligaments from a study group need to be loaded in order to adequately determine their collective viscoelastic behavior. Rat ligaments were subjected either to creep or stress relaxation for 1,000 s or stress relaxation for 10,000 s to compare estimates ...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Manley E Jr,Provenzano PP,Heisey D,Lakes R,Vanderby R Jr

    更新日期:2003-01-01 00:00:00

  • Tensile behavior and extensional viscosity of bile.

    abstract:BACKGROUND:Bile, which is secreted by the liver, is essential for digesting fat and maintaining homeostasis. Although the rheology of bile is very important to its flow, its extensional viscosity has not been studied. OBJECTIVE:We investigated the stretching of bile and evaluated its extensional viscosity using a fila...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-190216

    authors: Minh NN,Obara H,Shimokasa K,Zhu J

    更新日期:2019-01-01 00:00:00

  • Erythrocytes sedimentation profiles under gravitational field as determined by He-Ne laser. VII. Influence of dextrans, albumin and saline on cellular aggregation and sedimentation rate.

    abstract::The erythrocytes sedimentation profiles (ESP) of normal blood and of blood mixed with saline, albumin (7%), and various molecular weight dextrans of different concentrations, at various height and widths of the sample holder are determined. These observations show that the sedimentation characteristics of the erythroc...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1987-24106

    authors: Singh M,Joseph KP

    更新日期:1987-01-01 00:00:00

  • An inflammation-responsive transcription factor in the pathophysiology of osteoarthritis.

    abstract::A number of risk factors including biomechanical stress on the articular cartilage imposed by joint overloading due to obesity, repetitive damage of the joint tissues by injury of the menisci and ligaments, and abnormal joint alignment play a significant role in the onset of osteoarthritis (OA). Genetic predisposition...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Ray A,Ray BK

    更新日期:2008-01-01 00:00:00

  • Remodelling of the zero-stress state and residual strains in apoE-deficient mouse aorta.

    abstract::Atherosclerosis is the most frequent cause of death and severe chronic disability in North America and Europe. The atherosclerosis-prone apolipoprotein E (apoE)-deficient mice contain the entire spectrum of lesions observed during atherogenesis. Significant remodelling of the artery occurs in atherosclerosis. The aim ...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Gregersen H,Zhao J,Lu X,Zhou J,Falk E

    更新日期:2007-01-01 00:00:00

  • Structure of blood flow through a curved vessel with an aneurysm.

    abstract::A fine structure of blood flow through a curved vessel with an aneurysm was studied in in vitro experiments in relation to rheological factors of arterial diseases such as arteriosclerosis or thrombosis. On the basis of the in vivo data related to cerebral circulation, red blood cell suspension was flowed through curv...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1984-21418

    authors: Niimi H,Kawano Y,Sugiyama I

    更新日期:1984-01-01 00:00:00

  • The influence of repair tissue maturation on the response to oscillatory compression in a cartilage defect repair model.

    abstract::This study examined the effects of mechanical compression on engineered cartilage in a novel hybrid culture system. Cylindrical holes were cut in discs of bovine articular cartilage and filled with agarose gels containing chondrocytes. These constructs were compressed in radiolabeled medium under static or oscillatory...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Hunter CJ,Levenston ME

    更新日期:2002-01-01 00:00:00

  • Non-Newtonian viscosity of human blood: flow-induced changes in microstructure.

    abstract::Flow-induced changes in the red cell microstructure of human blood are identified from mechanical and optical evidence. On initiation of steady flow, a new microstructure develops as the shear strain increases through unit strain. This structure is identified with the formation of layers of red cells that slide on pla...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1994-31206

    authors: Thurston GB

    更新日期:1994-03-01 00:00:00

  • Simultaneous monitoring of electrical conductance and light transmittance during red blood cell aggregation.

    abstract::The electrical properties of red blood cell (RBC) suspensions are influenced by flow conditions, and prior studies indicate that electrical properties may reflect the kinetics of RBC aggregation. Changes of conductance and capacitance were monitored and had a time course resembling a "syllectogram" (i.e., temporal cha...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-2009-0541

    authors: Baskurt OK,Uyuklu M,Meiselman HJ

    更新日期:2009-01-01 00:00:00

  • The influence of L-sorbose on red cell flow properties, shape and packing ability.

    abstract::Since the sweet ketohexose L-sorbose causes overt hemolysis in dogs but not in man, we examined the possibility that L-sorbose induces a "prehemolytic state" of human red cells, manifesting itself as impairment of rheological red cell properties. After 2 hours incubation at 37 degrees C relative viscosity of red cell ...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1985-22302

    authors: Stäubli M,Wälchli P,Straub PW

    更新日期:1985-01-01 00:00:00

  • Influence of parenteral nutrition on blood rheology and platelet aggregation in vitro.

    abstract:BACKGROUND:Parenteral nutrition (PN) is a hyperosmolar solution composed of glucose, amino acids and a lipid emulsion, which is often used despite well-known side effects and complications. OBJECTIVES:In this study the hypothesis was tested that PN could affect hemorheology. METHODS:The influence of increasing plasma...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-140652

    authors: Compagnoni SC,Schulzki T,Thoeny S,Reinhart WH

    更新日期:2014-01-01 00:00:00

  • Study of red cell aggregation in pulsatile flow from ultrasonic Doppler power measurements.

    abstract::Human red cell aggregability and disaggregability represent important hemorheological parameters of blood. Several techniques have been proposed to evaluate the tendency of red cells to form aggregates and to disrupt in the presence of shear stress. One of the most recent approaches is based on the characterization of...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1993-305-615

    authors: Cloutier G,Shung KK

    更新日期:1993-09-01 00:00:00

  • Computational study of the effect of geometric and flow parameters on the steady flow field at the rabbit aorto-celiac bifurcation.

    abstract::Arterial hemodynamic forces may play a role in the localization of early atherosclerotic lesions. We have been developing numerical techniques based on overset or "Chimera" type formulations to solve the Navier-Stokes equations in complex geometries simulating arterial bifurcations. This paper presents three-dimension...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.1016/s0006-355x(99)80020-1

    authors: Cheer AY,Dwyer HA,Barakat AI,Sy E,Bice M

    更新日期:1998-11-01 00:00:00

  • Platelet near-wall excess in porcine whole blood in artery-sized tubes under steady and pulsatile flow conditions.

    abstract::Platelet margination (enhanced platelet concentration in the near wall region of a blood vessel) has been well documented in small vessels. In artery-sized vessels margination has only been demonstrated in one study, using ghost cell suspensions and under relatively non-physiologic conditions of steady flow and 50 cm ...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Xu C,Wootton DM

    更新日期:2004-01-01 00:00:00

  • Biorheology and fluid flux in swelling tissues. I. Bicomponent theory for small deformations, including concentration effects.

    abstract::A theory for the rheological behavior and fluid flux in swelling tissues under small deformations is presented. Tissues are considered as bicomponent solid-fluid mixtures. Concentration effects are included. The driving forces (body, surface and interactive), are discussed and their constitutive relationships to the t...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1987-24210

    authors: Lanir Y

    更新日期:1987-01-01 00:00:00

  • Cross-sectional distributions of normal and abnormal red blood cells in capillary tubes determined by a new technique.

    abstract:BACKGROUND:In the microcirculation, red blood cells (RBCs) were observed to be confined to an axial stream surrounded by a marginal RBC depleted layer. This axial accumulation of RBCs is considered to arise from the RBC deformability. OBJECTIVE:To quantitatively evaluate the effect of RBC deformability on their axial ...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-18166

    authors: Sasaki T,Seki J,Itano T,Sugihara-Seki M

    更新日期:2018-01-01 00:00:00

  • Rheological properties and wall structures of large veins.

    abstract::The static and dynamic viscoelastic properties were studied of longitudinal and circumferential strips excised from various large veins of dogs. The mechanical behavior in longitudinal direction could be regarded as elastic, while that in circumferential direction was highly viscoelastic. No distinct regionality was o...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1983-20510

    authors: Hasegawa M

    更新日期:1983-01-01 00:00:00

  • Osteoarthritic synovial fluid rheology and correlations with protein concentration.

    abstract:BACKGROUND:Osteoarthritis is a common, localized joint disease that causes pain, stiffness and reduced mobility. Osteoarthritis is particularly common in the knees. The effects of osteoarthritis on the rheology of synovial fluid in the knees are not fully understood and consequently require further study. OBJECTIVE:Th...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-15078

    authors: Madkhali A,Chernos M,Grecov D,Kwok E

    更新日期:2016-11-09 00:00:00

  • Shear linear behavior of brain tissue over a large frequency range.

    abstract::The literature review about the shear linear properties of brain tissue reveals both a large discrepancy in the existing data and a crucial lack of information at high frequencies associated with traffic road and non-penetrating ballistic impacts. The purpose of this study is to clarify and to complement the linear ma...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:

    authors: Nicolle S,Lounis M,Willinger R,Palierne JF

    更新日期:2005-01-01 00:00:00

  • Plasma fractionation by dead-end membrane filtration: effects of membrane properties and plasma flux.

    abstract::Plasma fractionation by membrane filtration permits the reinfusion of the patient with his own albumin. In this study, the influence of membrane nature and plasma flux on plasma fractionation in dead-end mode is investigated with acetate hollow fiber filters. It is found that transmembrane pressure TMP rises exponenti...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1991-281-204

    authors: Charcosset C,Ding LH,Jaffrin MY

    更新日期:1991-01-01 00:00:00

  • Endothelial surface glycocalyx (ESG) components and ultra-structure revealed by stochastic optical reconstruction microscopy (STORM).

    abstract:BACKGROUND:In order to play different roles in vascular functions as a mechanosensor to blood flows and as a barrier to transvascular exchange, the endothelial surface glycocalyx (ESG) should have an organized structure. Due to the limitations of optical and electron microscopy, the ultra-structure of ESG has not been ...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-180204

    authors: Fan J,Sun Y,Xia Y,Tarbell JM,Fu BM

    更新日期:2019-01-01 00:00:00

  • Interactive role of tyrosine kinase, protein kinase C, and Rho/Rho kinase systems in the mechanotransduction of vascular smooth muscles.

    abstract::Blood vessels are always subjected to hemodynamic stresses including blood pressure and blood flow. The cerebral artery is particularly sensitive to hemodynamic stresses such as pressure and stretch, and shows contractions that are myogenic in nature; i.e., the mechanical response is generated by the vascular smooth m...

    journal_title:Biorheology

    pub_type: 杂志文章,评审

    doi:

    authors: Nakayama K,Obara K,Tanabe Y,Saito M,Ishikawa T,Nishizawa S

    更新日期:2003-01-01 00:00:00

  • Contactless cell trapping by the use of a uniform AC electric field.

    abstract::The AC electric field-driven manipulation of suspended polarizable particles has become a major technique in micro- and nano-devices. In the present study, suspensions of cultured HeLa cells in isotonic solution were used to explore the mechanisms underlying the suspension behaviors during exposure to a uniform AC ele...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-130644

    authors: Tada S,Natsuya T,Tsukamoto A,Santo Y

    更新日期:2013-01-01 00:00:00

  • Model studies of leukocyte-endothelium-blood interactions. I. The fluid flow drag force on the adherent leukocyte.

    abstract::Using a large scale model and the concept of dimensional similarity, we have developed a dimensionless correlation which permits the estimation of the drag force of blood flow in an in vivo vessel on a leukocyte adherent to the vessel wall. This relationship is [formula: see text] where Re is a Reynolds number, Cd is ...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.1016/0006-355X(96)00011-X

    authors: Chapman G,Cokelet G

    更新日期:1996-03-01 00:00:00

  • Fission and refusion of red blood cells using a micropipette technique.

    abstract::In micropipette experiments with small capillaries and moderate high pressure difference (approximately 1000 Pa) cell fragmentation (fission) of human red blood cells without hemolysis was observed by TV-system for a large number of fresh red blood cells of different donors. After separation, the fragment moves away f...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1987-24302

    authors: Meier W,Lerche D,Wendt MO,Paulitschke M

    更新日期:1987-01-01 00:00:00

  • Arterial deformation with renal artery aneurysm as a basis for secondary hypertension.

    abstract::The goal of this work is to better understand the association between renal artery aneurysms and secondary hypertension through modeling the blood flow and the mechanics of diseased arteries. A large number of patients with renal artery aneurysms exhibit hypertension. Following surgical intervention, some patients exp...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-130623

    authors: Down LA,Papavassiliou DV,O'Rear EA

    更新日期:2013-01-01 00:00:00

  • Effects of concentration and structure on proteoglycan 4 rheology and interaction with hyaluronan.

    abstract:BACKGROUND:The contribution of proteoglycan 4 (PRG4) to synovial fluid and hyaluronan (HA) solution rheology are poorly understood. The effects of PRG4 disulfide-bonded structure on viscosity and viscosity of newly available full-length recombinant human PRG4 (rhPRG4) have not previously been reported. OBJECTIVE:This ...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/BIR-14037

    authors: Ludwig TE,Cowman MK,Jay GD,Schmidt TA

    更新日期:2014-01-01 00:00:00

  • Rheology of the vitreous body. Part I: Viscoelasticity of human vitreous.

    abstract::The rheological properties of the vitreous body of the eye are believed to be a function of composition and to differ among species, as well as to vary regionally within the vitreous. These properties are essential to the mechanical functioning of the eye. Although there are gross, qualitative data on vitreous rheolog...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1992-295-612

    authors: Lee B,Litt M,Buchsbaum G

    更新日期:1992-09-01 00:00:00

  • Microvessel diameter estimation: error bias correction of serial measurements.

    abstract::The assessment of vessel patency can be substantially improved by serial microvessel diameter measurements taken successively along an extensive length of the vessel. It is possible to avoid making the a priori assumptions about the existence or location of local constriction sites implicit in single diameter measurem...

    journal_title:Biorheology

    pub_type: 杂志文章

    doi:10.3233/bir-1991-283-420

    authors: Miles FP,Nuttall AL

    更新日期:1991-01-01 00:00:00