Abstract:
:Both chondrocytes and mensenchymal stem cells (MSCs) are the most used cell sources for cartilage tissue engineering. However, monolayer expansion to obtain sufficient cells leads to a rapid chondrocyte dedifferentiation and a subsequent ancillary reduced ability of MSCs to differentiate into chondrocytes, thus limiting their application in cartilage repair. The aim of this study was to investigate the influence of the monolayer expansion on the immunophenotype and the gene expression profile of both cell types, and to find the appropriate compromise between monolayer expansion and the remaining chondrogenic characteristics. To this end, human chondrocytes, isolated enzymatically from femoral head slice, and human MSCs, derived from bone marrow, were maintained in monolayer culture up to passage 5. The respective expressions of cell surface markers (CD34, CD45, CD73, CD90, CD105, CD166) and several chondrogenic-related genes for each passage (P0-P5) of those cells were then analyzed using flow cytometry and quantitative real-time PCR, respectively. Flow cytometry analyses showed that, during the monolayer expansion, some qualitative and quantitative regulations occur for the expression of cell surface markers. A rapid increase in mRNA expression of type 1 collagen occurs whereas a significant decrease of type 2 collagen and Sox 9 was observed in chondrocytes through the successive passages. On the other hand, the expansion did not induced obvious change in MSCs gene expression. In conclusion, our results suggest that passage 1 might be the up-limit for chondrocytes in order to achieve their subsequent redifferentiation in 3D scaffold. Nevertheless, MSCs could be expanded in monolayer until passage 5 without loosing their undifferentiated phenotypes.
journal_name
Biorheologyjournal_title
Biorheologyauthors
Cournil-Henrionnet C,Huselstein C,Wang Y,Galois L,Mainard D,Decot V,Netter P,Stoltz JF,Muller S,Gillet P,Watrin-Pinzano Asubject
Has Abstractpub_date
2008-01-01 00:00:00pages
513-26issue
3-4eissn
0006-355Xissn
1878-5034journal_volume
45pub_type
杂志文章相关文献
BIORHEOLOGY文献大全abstract::A theory for the rheological behavior and fluid flux in swelling tissues under small deformations is presented. Tissues are considered as bicomponent solid-fluid mixtures. Concentration effects are included. The driving forces (body, surface and interactive), are discussed and their constitutive relationships to the t...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1987-24210
更新日期:1987-01-01 00:00:00
abstract::Several noninvasive light scattering techniques are reviewed that can be utilized in rheological studies of mucus and other soft biological matrices. Included are quasielastic light scattering techniques to determine the compressibility moduli of polymer lattices, resonance methods that can be used to measure the shea...
journal_title:Biorheology
pub_type: 杂志文章,评审
doi:10.3233/bir-1987-24609
更新日期:1987-01-01 00:00:00
abstract::Stress relaxation in three varieties of cherries was studied using the penetration test with a cylindrical pin, 4 mm in diameter, having a flat tip. One half of the fruit was modified by partial skinning, since experiments show that skin approximately doubles the apparent modulus of elasticity of the cherries. The ski...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.1016/s0006-355x(97)00033-4
更新日期:1996-11-01 00:00:00
abstract::The maintenance of articular cartilage integrity requires a balance between anabolic and catabolic processes which are under the control of chondrocytes. These cells are living in an anaerobic environment and normally do not divide. They are responsible for the continuous maintenance of the cartilage extracellular mat...
journal_title:Biorheology
pub_type: 杂志文章,评审
doi:
更新日期:2000-01-01 00:00:00
abstract::Over the past several decades, blood-soluble drag reducing polymers (DRPs) have been shown to significantly enhance hemodynamics in various animal models when added to blood at nanomolar concentrations. In the present study, the effects of the DRPs on blood circulation were tested in anesthetized rats exposed to acute...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:Red blood cell (RBC) deformability may increase, or decrease, following application of shear stress ("shear conditioning"), depending upon the specific magnitude and duration of exposure. However, the time course of altered RBC deformability following shear remains unresolved. OBJECTIVE:We utilised shear co...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-17151
更新日期:2018-01-01 00:00:00
abstract::Frozen, stored mucus has been extensively used for transport studies but there is no clear evidence of the influence that the temperature and length of time of storage may have on the results. We stored frog mucus samples at -20 and -80 degrees C and analysed them on days zero, 2, 10, 30 and 90. At each temperature, a...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::The supply of oxygen to tissue from capillaries may be influenced by hemorheological factors. A hemorheological model is developed for elucidating which factors play a major role in the oxygen transport to tissue. For a capillary tissue unit, a modified cylindrical model of Krogh is used. For blood, two fluids model o...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1983-20517
更新日期:1983-01-01 00:00:00
abstract:BACKGROUND:Leukocytes and platelets typically fulfil their functions through adhesion to the walls of vessels with different size, haematocrit and shear rate. OBJECTIVE:We aimed to investigate differential effects of these variables on leukocyte and platelet adhesion. METHODS:Blood with varying haematocrit was perfus...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-15043
更新日期:2015-01-01 00:00:00
abstract::Different methods are commonly used to study the red blood cell aggregation phenomenon. The major interest of the ultrasonic method presently discussed is to assess the mean size of red blood cell (RBC) aggregates by measuring ultrasonic intensity backscattered by blood. Applying Rayleigh theory of sound to blood medi...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1990-27104
更新日期:1990-01-01 00:00:00
abstract::Ciliary metachronism and motility were examined optically in muco-ciliary tissue cultures from three different systems: a) frog's palate epithelium, b) frog's oesophagus, and c) human nasal polyps. In addition, lateral cilia of Mytilus edulis (water transporting cilia) were examined. It was revealed that the degree of...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1990-273-433
更新日期:1990-01-01 00:00:00
abstract::The literature review about the shear linear properties of brain tissue reveals both a large discrepancy in the existing data and a crucial lack of information at high frequencies associated with traffic road and non-penetrating ballistic impacts. The purpose of this study is to clarify and to complement the linear ma...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::Magnitude and time-dependence of the effects of red cell aggregation and sedimentation on the rheology of human blood were studied during low shear (tau W 2.5 to 92 mPa) flow through horizontal tubes (ID 25 to 105 microns). Immediately following reduction of perfusion pressure to a low value the red cell concentration...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1989-26210
更新日期:1989-01-01 00:00:00
abstract::The effects of platelet margination and enhanced platelet diffusivity, as induced by red blood cells, on the adhesion of platelets, were investigated for a range of haematocrits, under 2D axi-symmetric flow, simulating previous in vitro experiments [Microvasc. Res. 17 (1979), 238-262]. The effect of margination was in...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:Postmenopausal women often develop hemorheological disorders which may affect the systemic blood circulation and present a cardiovascular risk factor. OBJECTIVE:We evaluated effects of secoisolariciresinol (SECO), a phytoestrogen, on hemorheological parameters and lipid peroxidation in a model of the age-re...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-15066
更新日期:2016-01-07 00:00:00
abstract::In micropipette experiments with small capillaries and moderate high pressure difference (approximately 1000 Pa) cell fragmentation (fission) of human red blood cells without hemolysis was observed by TV-system for a large number of fresh red blood cells of different donors. After separation, the fragment moves away f...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1987-24302
更新日期:1987-01-01 00:00:00
abstract::It has long been recognized that understanding the rheological properties of blood is essential to a full understanding of the function of the circulatory system. Given the difficulty of obtaining carefully controlled measurements in vivo, most of our current concepts of the flow behavior of blood in vivo are based on...
journal_title:Biorheology
pub_type: 杂志文章,评审
doi:
更新日期:2001-01-01 00:00:00
abstract::In channel flow of multicomponent suspensions, segregation behavior of suspended components perpendicular to the flow direction is often observed, which is considered to be caused by the differential properties of the lateral migration depending on their shape, size, flexibility, and other characteristics. In the pres...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-130638
更新日期:2013-01-01 00:00:00
abstract::Under some conditions, such as inhalation injury, an array of longitudinal channels without cilia may be formed on the epithelial surface, affecting mucus transport in the lung during coughing. Moreover, in certain cases of the diseased state of the lung, immotile cilia remain embedded in the serous fluid and may form...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1994-31102
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:Osteoarthritis is a common, localized joint disease that causes pain, stiffness and reduced mobility. Osteoarthritis is particularly common in the knees. The effects of osteoarthritis on the rheology of synovial fluid in the knees are not fully understood and consequently require further study. OBJECTIVE:Th...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-15078
更新日期:2016-11-09 00:00:00
abstract::The role of clustered of L-selectin receptors on the leukocyte surface is discussed in connection with the postulated velocity-dependent formation of selectin-ligand tether bonds to interpret the mechanism of leukocyte tethering to, and rolling along, the vascular endothelium. The distinct feature of this step-wise pr...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::A non-biodegradable polyhedral oligomeric silsesquioxane (POSS) nanocomposite biopolymer has been developed for fabrication of medical devices and for tissue engineering human organs. The polymer in solution, containing 2 wt% of POSS, has been synthesized, characterized and investigated to determine its key rheologica...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::Human red cell aggregability and disaggregability represent important hemorheological parameters of blood. Several techniques have been proposed to evaluate the tendency of red cells to form aggregates and to disrupt in the presence of shear stress. One of the most recent approaches is based on the characterization of...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1993-305-615
更新日期:1993-09-01 00:00:00
abstract::The aim of this study was to investigate the effect of hydrostatic pressure on the expression of messenger ribonucleic acid (mRNA) for specific extracellular matrix proteins in chondrocytes. Chondrocytes obtained from bovine metatarsophalangeal joints were embedded in cylindrical 2% agarose gels. A novel experimental ...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::During the recent years various investigators have developed the technique of kryophotometric measurements of oxyhemoglobin saturation with oxygen (psi) in small vessels. In this paper, on the basis of the statistical model of the organ developed by the authors, a theoretical relationship is established between the di...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1982-191-235
更新日期:1982-01-01 00:00:00
abstract::Arterial hemodynamic forces may play a role in the localization of early atherosclerotic lesions. We have been developing numerical techniques based on overset or "Chimera" type formulations to solve the Navier-Stokes equations in complex geometries simulating arterial bifurcations. This paper presents three-dimension...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.1016/s0006-355x(99)80020-1
更新日期:1998-11-01 00:00:00
abstract::It is has been known for more than 80 years that compared to in vitro determinations, blood behaves as a less viscous fluid under in vivo flow conditions. The experiments of Whittaker and Winton were among the first dealing with the in vivo effects of altered blood rheology, and experimental studies during the second ...
journal_title:Biorheology
pub_type: 杂志文章,评审
doi:
更新日期:2008-01-01 00:00:00
abstract::The assessment of vessel patency can be substantially improved by serial microvessel diameter measurements taken successively along an extensive length of the vessel. It is possible to avoid making the a priori assumptions about the existence or location of local constriction sites implicit in single diameter measurem...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1991-283-420
更新日期:1991-01-01 00:00:00
abstract::Mechanical forces can stimulate the production of extracellular matrix molecules. We tested the efficacy of ultrasound to increase proteoglycan synthesis in bovine primary chondrocytes. The ultrasound-induced temperature rise was measured and its contribution to the synthesis was investigated using bare heat stimulus....
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Conducting experiments on very soft biological tissues can be difficult. Traditionally, unconfined compression and shear have been used. Here, an improved method of compression testing, lubricated squeezing flow is described. This gives a uniform compression along the squeezing axis and almost uniform equi-biaxial elo...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00