Abstract:
:ABSTRACT Digestive cysteine proteinases have been isolated from plant-parasitic nematodes as well as coleopteran and hemipteran insects. Phytocystatins, inhibitors of cysteine proteinases, are found in a number of plants where they may play a role in defense against pathogens and pests. The cDNAs of the phytocystatins from rice, oryzacystatin I (OC-I) and oryzacystatin II (OC-II), were expressed in alfalfa (Medicago sativa) plants under the control of the potato protease inhibitor II (PinII) promoter and the plants were evaluated for resistance to the root-lesion nematode (Pratylenchus penetrans). A PinII-beta-glucuronidase (GUS) gene was introduced into alfalfa to determine the pattern of gene expression from this promoter. Constitutive GUS expression was observed in leaf and root vascular tissue, and in some plants, expression was observed in leaf mesophyll cells. Mechanical wounding of leaves increased GUS expression approximately twofold over 24 h. Inoculation with root-lesion nematodes resulted in localized GUS expression. Populations of root-lesion nematodes in alfalfa roots from one line containing the PinII::OC-I transgene and one line containing the PinII::OC-II transgene were reduced 29 and 32%, respectively, compared with a transgenic control line. These results suggest that oryzacystatins have the potential to confer increased resistance to the root-lesion nematode in alfalfa.
journal_name
Phytopathologyjournal_title
Phytopathologyauthors
Samac DA,Smigocki ACdoi
10.1094/PHYTO.2003.93.7.799subject
Has Abstractpub_date
2003-07-01 00:00:00pages
799-804issue
7eissn
0031-949Xissn
1943-7684journal_volume
93pub_type
杂志文章相关文献
PHYTOPATHOLOGY文献大全abstract::ABSTRACT Apple fruit tissues infected with Botryosphaeria dothidea were examined by transmission electron microscopy using susceptible cv. Fuji and resistant cv. Jonathan. Immature (green) and mature (red) fruits of cv. Fuji with restricted or expanding lesions were also examined to reveal subcellular characteristics ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2001.91.2.165
更新日期:2001-02-01 00:00:00
abstract::ABSTRACT Crown rust (Puccinia coronata) in indigenous populations of Avena sterilis has been cited as an example of stability of wild pathosystems that consist of natural mixtures of resistance and virulence. This study confirmed that virulence/avirulence polymorphisms in P. coronata on A. sterilis in Israel are highl...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2004.94.5.505
更新日期:2004-05-01 00:00:00
abstract::The study of the transmission biology of insect-borne plant viruses is important to develop disease control practices. We characterized the transmission of a nanovirus, Banana bunchy top virus (BBTV), by its aphid vector Pentalonia nigronervosa Coquerel (Hemiptera, Aphididae) with respect to temperature, vector life s...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-98-6-0743
更新日期:2008-06-01 00:00:00
abstract::Citrus canker, caused by Xanthomonas citri ssp. citri, is a serious disease that causes substantial economic losses to the citrus industry worldwide. The bactericide bismerthiazol has been used to control rice bacterial blight (X. oryzae pv. oryzae). In this paper, we demonstrate that bismerthiazol can effectively con...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-12-15-0328-R
更新日期:2016-07-01 00:00:00
abstract::ABSTRACT Genetic resistance often fails because a resistance-breaking (RB) pathogen genotype increases in frequency. On the basis of an analysis of cellular plant pathogens, it was recently proposed that the evolutionary potential of a pathogen is a major determinant of the durability of resistance. We test this hypot...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2003.93.8.941
更新日期:2003-08-01 00:00:00
abstract::ABSTRACT Plasmopara viticola populations collected from three islands in the Ionian Sea-an arm of the Mediterranean Sea to the west of Greece-were analyzed with microsatellite molecular markers in order to investigate the pathogen population structure. Downy mildew populations from mainland regions previously studied ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-96-0501
更新日期:2006-05-01 00:00:00
abstract::ABSTRACT Fusarium verticillioides, F. proliferatum, and Aspergillus flavus cause ear rots of maize and contaminate the grain with mycotoxins (fumonisin or aflatoxin). The objective of this study was to investigate the relationships between resistance to Fusarium and Aspergillus ear rots and fumonisin and aflatoxin con...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-97-3-0311
更新日期:2007-03-01 00:00:00
abstract::Moroccan watermelon mosaic virus (MWMV) represents an emerging threat to cucurbit production in the Mediterranean Basin. We sequenced the near complete genome of MWMV-SQ10_1.1, a cloned Spanish isolate. MWMV-SQ10_1.1 has the typical potyvirus genomic structure, and phylogenetic analysis showed that it shared a common ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-10-18-0395-R
更新日期:2019-05-01 00:00:00
abstract::ABSTRACT White root rot, caused by Rosellinia necatrix, is a serious soilborne disease of fruit trees and other woody plants. R. necatrix isolate W370 contains 12 segments of double-stranded RNA (dsRNA) that is believed to represent a possible member of the family Reoviridae. W370 was weakly virulent and its hyphal-ti...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2004.94.6.561
更新日期:2004-06-01 00:00:00
abstract::ABSTRACT Monoconidial isolates of Venturia inaequalis were collected in 1990 and 1991 from orchards in New York, Michigan, and Nova Scotia that had never or only sporadically been treated with fungicides acting as sterol demethylation inhibitors (DMIs). Sensitivities of isolates to two representative DMIs (fenarimol a...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1997.87.2.184
更新日期:1997-02-01 00:00:00
abstract::We report the detection of the huanglongbing (HLB)-associated bacterium 'Candidatus Liberibacter asiaticus' from both plants and insects in Pakistan and the seasonal variability in the numbers of 'Ca. L. asiaticus'-positive psyllid vector, Diaphorina citri. Our studies showed that 'Ca. L. asiaticus' was detectable fro...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-08-13-0224-R
更新日期:2014-03-01 00:00:00
abstract::Raffaelea lauricola, a fungal symbiont of the ambrosia beetle Xyleborus glabratus, causes laurel wilt in members of the Lauraceae plant family. North American species in the family, such as avocado (Persea americana) and swamp bay (P. palustris), are particularly susceptible to laurel wilt, whereas the Asian camphortr...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-02-16-0072-R
更新日期:2017-01-01 00:00:00
abstract::To assess the diversity of Xanthomonas campestris spp. infecting crucifers in New York, 154 isolates were collected over 10 years across the state. The goal was to determine if isolates of the pathogen were overwintering in New York and serving as primary inoculum in subsequent years, or if novel isolates were enterin...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-06-15-0134-R
更新日期:2016-02-01 00:00:00
abstract::The role of Grapevine Pinot gris virus (GPGV) in the etiology of grapevine leaf mottling and deformation was investigated by biological and molecular assays. A survey on different cultivars from the Trentino Region in Italy showed a widespread distribution of GPGV, which was associated with symptomatic (79%) but also ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-09-14-0241-R
更新日期:2015-04-01 00:00:00
abstract::Bean common mosaic virus (BCMV) exists as a complex of strains classified by reactions to resistance genes found in common bean (Phaseolus vulgaris); seven BCMV pathotypes have been distinguished thus far, numbered I to VII. Virus genetic determinants involved in pathogenicity interactions with resistance genes have n...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-08-13-0243-R
更新日期:2014-07-01 00:00:00
abstract::To support the search for alternative, non-chemical plant disease control strategies, we present a review of the pathogen-suppressive effects of biochar, a product derived from agricultural and other organic wastes, used as a soil amendment. A wide range of biochar effects contribute to the control of root or foliar f...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-06-20-0248-RVW
更新日期:2021-02-02 00:00:00
abstract::ABSTRACT The contribution of gluconic acid secretion to the colonization of apple tissue by Penicillium expansum was analyzed by modulation (increase or decrease) of gluconic acid accumulation at the infection court. P. expansum isolates that express the most gox2 transcripts and concomitant glucose oxidase (GOX) acti...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-97-3-0384
更新日期:2007-03-01 00:00:00
abstract::The Fusarium oxysporum species complex includes both plant pathogenic and nonpathogenic strains, which are commonly found in soils. F. oxysporum has received considerable attention from plant pathologists for more than a century owing to its broad host range and the economic losses it causes. The narrow host specifici...
journal_title:Phytopathology
pub_type: 杂志文章,评审
doi:10.1094/PHYTO-08-18-0320-RVW
更新日期:2019-04-01 00:00:00
abstract::ABSTRACT Locations of silicon accumulation in rice leaves and its possible association with resistance to rice blast were investigated by electron microscopy and X-ray microanalysis. A blast-susceptible cultivar, Jinmi, and a partially resistant cultivar, Hwaseong, were grown under a hydroponic culture system with mod...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2002.92.10.1095
更新日期:2002-10-01 00:00:00
abstract::ABSTRACT Suppressive effects of soil amendment with residues of 12 cultivars of Brassica rapa on damping-off of sugar beet were evaluated in soils infested with Rhizoctonia solani. Residues of clover and peanut were tested as noncruciferous controls. The incidence of damping-off was significantly and consistently supp...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-96-1372
更新日期:2006-12-01 00:00:00
abstract::Murraya paniculata and Swinglea glutinosa are aurantioid hosts of the Asian citrus psyllid (ACP) Diaphorina citri, the principal vector of 'Candidatus Liberibacter asiaticus' (Las). Las is the pathogen associated with huanglongbing (HLB), the Asian form of which is the most devastating disease of Citrus species and cu...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-06-19-0216-R
更新日期:2019-12-01 00:00:00
abstract::ABSTRACT None of the chemical alternatives currently registered and available has the full spectrum of activity and versatility of methyl bromide as a pre-plant soil fumigant. Chloropicrin and 1,3-dichloropropene (Telone) can provide significant control of many plant pathogens in soil and growth stimulation in annual ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2002.92.12.1337
更新日期:2002-12-01 00:00:00
abstract::Compartmental models have become the dominant theoretical paradigm in mechanistic modeling of plant disease and offer well-known advantages in terms of analytic tractability, ease of simulation, and extensibility. However, underlying assumptions of constant rates of infection and of exponentially distributed latent an...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-12-10-0338
更新日期:2012-04-01 00:00:00
abstract::ABSTRACT Genes for copper resistance, located on the chromosome of strain XvP26 of Xanthomonas axonopodis pv. vesicatoria, were transferred by conjugation to a recipient strain of the bacterium. The chromosomal gene transfer was verified by analyses of the genomes of donor, recipient, and putative transconjugants for ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.1999.89.11.1044
更新日期:1999-11-01 00:00:00
abstract::Limber pine (Pinus flexilis) is being threatened by the lethal disease white pine blister rust caused by the non-native pathogen Cronartium ribicola. The types and frequencies of genetic resistance to the rust will likely determine the potential success of restoration or proactive measures. These first extensive inocu...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-04-13-0092-R
更新日期:2014-02-01 00:00:00
abstract::Ralstonia solanacearum causes bacterial wilt on a wide range of plant hosts. Most strains of R. solanacearum are nonpathogenic below 20°C; however, Race 3 Biovar 2 (R3B2) strains are classified as quarantine pathogens because of their ability to infect crops, cause disease, and survive in temperate climates. We have i...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-05-11-0145
更新日期:2012-02-01 00:00:00
abstract::Understanding pathogen evolution over time is vital for plant breeding and deployment of host resistance. In the context of a soilborne pathogen, the potential of host-directed evolution of a Verticillium dahliae race 1 isolate and genotypic variation of V. dahliae associated with two major hosts (lettuce and tomato) ...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-02-17-0071-R
更新日期:2017-11-01 00:00:00
abstract::Although 'Candidatus Liberibacter asiaticus' (Las) is a major pathogen associated with citrus huanglongbing (HLB), some characteristics of transmission by the psyllid vector Diaphorina citri are not fully understood. We examined the latent period and persistence of transmission of Las by D. citri in a series of experi...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-02-16-0088-R
更新日期:2017-03-01 00:00:00
abstract::ABSTRACT Two microtiter plate assays were developed to study the adherence of the plant-pathogenic mollicute Spiroplasma citri to a monolayer of cultured cells of its leafhopper vector, Circulifer tenellus. Adherence was significantly reduced by prior treatment of the spiroplasmas with proteinase K or pronase. Electro...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO.2000.90.7.716
更新日期:2000-07-01 00:00:00
abstract::Xanthomonas oryzae pv. oryzicola is an important bacterial pathogen responsible for outbreaks of bacterial leaf streak (BLS) on rice, mostly occurring in Asia and parts of Africa. To better monitor epidemics and assess population structures, efficient tools that allow the precise identification and diagnosis of pathog...
journal_title:Phytopathology
pub_type: 杂志文章
doi:10.1094/PHYTO-04-12-0078-R
更新日期:2012-10-01 00:00:00