VirE1-Mediated Resistance to Crown Gall in Transgenic Arabidopsis thaliana.

Abstract:

:ABSTRACT Crown gall disease, caused by Agrobacterium tumefaciens, remains a serious agricultural problem despite current biocontrol methods. Agrobacterium tumefaciens transfers single-stranded DNA (T-strands) into plant cells along with several virulence proteins, including a single-stranded DNA-binding protein (VirE2). In plant cells, T-strands are protected from nucleases and targeted to the nucleus by VirE2, which is essential for efficient transmission (transfer and integration) of T-strands. VirE1 is the secretory chaperone for VirE2; it prevents VirE2 from forming aggregates and from binding the T-strands in bacterial cells. Therefore, we hypothesized that sufficient quantities of VirE1 expressed in plant cells might block T-DNA transmission by preventing VirE2 from binding T-strands. Here we show that root explants from Arabidopsis thaliana plants that expressed virE1 formed 3.5-fold fewer tumors than roots from plants without virE1. Also, this resistance was specific for VirE2-mediated Agrobacterium transformation. Plants that have been genetically altered to resist crown gall may prove more effective than biological control.

journal_name

Phytopathology

journal_title

Phytopathology

authors

Humann J,Andrews S,Ream W

doi

10.1094/PHYTO-96-0105

subject

Has Abstract

pub_date

2006-01-01 00:00:00

pages

105-10

issue

1

eissn

0031-949X

issn

1943-7684

journal_volume

96

pub_type

杂志文章
  • Relationships between disease incidence at two levels in a spatial hierarchy.

    abstract::ABSTRACT Relationships between disease incidence measured at two levels in a spatial hierarchy are derived. These relationships are based on the properties of the binomial distribution, the beta-binomial distribution, and an empirical power-law relationship that relates observed variance to theoretical binomial varian...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1997.87.5.542

    authors: Hughes G,McRoberts N,Madden LV,Gottwald TR

    更新日期:1997-05-01 00:00:00

  • Transcriptome Profiling of Melaleuca quinquenervia Challenged by Myrtle Rust Reveals Differences in Defense Responses Among Resistant Individuals.

    abstract::Plants have developed complex defense mechanisms to protect themselves against pathogens. A wide-host-range fungus, Austropuccinia psidii, which has caused severe damage to ecosystems and plantations worldwide, is a major threat to Australian ecosystems dominated by members of the family Myrtaceae. In particular, the ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-09-17-0307-R

    authors: Hsieh JF,Chuah A,Patel HR,Sandhu KS,Foley WJ,Külheim C

    更新日期:2018-04-01 00:00:00

  • Riboflavin induces disease resistance in plants by activating a novel signal transduction pathway.

    abstract::ABSTRACT The role of riboflavin as an elicitor of systemic resistance and an activator of a novel signaling process in plants was demonstrated. Following treatment with riboflavin, Arabidopsis thaliana developed systemic resistance to Peronospora parasitica and Pseudomonas syringae pv. Tomato, and tobacco developed sy...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2000.90.8.801

    authors: Dong H,Beer SV

    更新日期:2000-08-01 00:00:00

  • Antagonism of the Two-Needle Pine Stem Rust Fungi Cronartium flaccidum and Peridermium pini by Cladosporium tenuissimum In Vitro and In Planta.

    abstract::ABSTRACT Selected isolates of Cladosporium tenuissimum were tested for their ability to inhibit in vitro aeciospore germination of the two-needle pine stem rusts Cronartium flaccidum and Peridermium pini and to suppress disease development in planta. The antagonistic fungus displayed a number of disease-suppressive me...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2001.91.5.457

    authors: Moricca S,Ragazzi A,Mitchelson KR,Assante G

    更新日期:2001-05-01 00:00:00

  • Molecular mapping of the major resistance quantitative trait locus qHS2.09 with simple sequence repeat and single nucleotide polymorphism markers in maize.

    abstract::The major quantitative trait locus (QTL) qHS2.09 plays an important role in resistance to head smut during maize breeding and production. In this study, a near-isogenic line (NIL), L34, which harbors the major QTL qHS2.09 in bin 2.09, was developed using a resistant donor 'Mo17' in a susceptible genetic background 'Hu...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-12-11-0330

    authors: Weng J,Liu X,Wang Z,Wang J,Zhang L,Hao Z,Xie C,Li M,Zhang D,Bai L,Liu C,Zhang S,Li X

    更新日期:2012-07-01 00:00:00

  • Pathogen variation and urea influence selection and success of Streptomyces mixtures in biological control.

    abstract::Success in biological control of plant diseases remains inconsistent in the field. A collection of well-characterized Streptomyces antagonists (n = 19 isolates) was tested for their capacities to inhibit pathogenic Streptomyces scabies (n = 15 isolates). There was significant variation among antagonists in ability to ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-06-12-0129-R

    authors: Otto-Hanson LK,Grabau Z,Rosen C,Salomon CE,Kinkel LL

    更新日期:2013-01-01 00:00:00

  • Development of a variable number of tandem repeats typing scheme for the bacterial rice pathogen Xanthomonas oryzae pv. oryzicola.

    abstract::Xanthomonas oryzae pv. oryzicola is an important bacterial pathogen responsible for outbreaks of bacterial leaf streak (BLS) on rice, mostly occurring in Asia and parts of Africa. To better monitor epidemics and assess population structures, efficient tools that allow the precise identification and diagnosis of pathog...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-04-12-0078-R

    authors: Zhao S,Poulin L,Rodriguez-R LM,Serna NF,Liu SY,Wonni I,Szurek B,Verdier V,Leach JE,He YQ,Feng JX,Koebnik R

    更新日期:2012-10-01 00:00:00

  • Calcium/Calmodulin-Dependent Signaling for Prepenetration Development in Colletotrichum gloeosporioides.

    abstract::ABSTRACT Colletotrichum gloeosporioides forms a specialized infection structure, an appressorium, for host infection. Contacting hard surface induces appressorium formation in C. gloeosporioides, whereas hydrophobicity of the contact surface does not affect this infection-related differentiation. To determine if the c...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2003.93.1.82

    authors: Uhm KH,Ahn IP,Kim S,Lee YH

    更新日期:2003-01-01 00:00:00

  • Effects of temperature on urediniospore germination, germ tube growth, and initiation of infection in soybean by phakopsora isolates.

    abstract::ABSTRACT Temperature is a critical factor in plant disease development. As part of a research program to determine how specific environmental variables affect soybean rust, we determined temperature effects on urediniospore germination and germ tube growth of four isolates of Phakopsora pachyrhizi, one each from Brazi...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-97-8-0997

    authors: Bonde MR,Berner DK,Nester SE,Frederick RD

    更新日期:2007-08-01 00:00:00

  • Genetic diversity of Potato virus Y infecting tobacco crops in China.

    abstract::Genetic variability of Potato virus Y (PVY) isolates infecting potato has been characterized but little is known about genetic diversity of PVY isolates infecting tobacco crops. In this study, PVY isolates were collected from major tobacco-growing areas in China and single-lesion isolates were produced by serial inocu...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-02-10-0055

    authors: Tian YP,Liu JL,Zhang CL,Liu YY,Wang B,Li XD,Guo ZK,Valkonen JP

    更新日期:2011-03-01 00:00:00

  • A Pectate Lyase Homolog, xagP, in Xanthomonas axonopodis pv. glycines Is Associated with Hypersensitive Response Induction on Tobacco.

    abstract::ABSTRACT Xanthomonas axonopodis pv. glycines is the causal agent of bacterial pustule disease of soybeans. A transposon insertional mutant (KU-P-M670) of X. axonopodis pv. glycines derived from wild-type strain KU-P-34017 lost the ability to induce the hypersensitive response (HR) on tobacco and pepper but retained it...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-96-1230

    authors: Kaewnum S,Prathuangwong S,Burr TJ

    更新日期:2006-11-01 00:00:00

  • Functional analysis of Pid3-A4, an ortholog of rice blast resistance gene Pid3 revealed by allele mining in common wild rice.

    abstract::The rice blast resistance gene Pid3 encodes a nucleotide-binding-site leucine-rich repeat (NBS-LRR) protein. This gene was cloned from the rice 'Digu' (indica) by performing a genome-wide comparison of the NBS-LRR gene family between two genome-sequenced varieties, '9311' (indica) and 'Nipponbare' (japonica). In this ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-10-12-0260-R

    authors: Lv Q,Xu X,Shang J,Jiang G,Pang Z,Zhou Z,Wang J,Liu Y,Li T,Li X,Xu J,Cheng Z,Zhao X,Li S,Zhu L

    更新日期:2013-06-01 00:00:00

  • Host diversity can reduce potato late blight severity for focal and general patterns of primary inoculum.

    abstract::ABSTRACT The use of host diversity as a tool for management of potato late blight has not been viewed as promising in the past. But the increasing importance of late blight internationally has brought new consideration to all potential management tools. We studied the effect of host diversity on epidemics of potato la...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2000.90.12.1307

    authors: Garrett KA,Mundt CC

    更新日期:2000-12-01 00:00:00

  • Description of the Nicotiana benthamiana-Cercospora nicotianae Pathosystem.

    abstract::Nicotiana benthamiana is a valuable model organism in plant biology research. This report describes its extended applicability in the field of molecular plant pathology by introducing a nonbiotrophic fungal pathogen Cercospora nicotianae that can be conveniently used under laboratory conditions, consistently induces a...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-12-16-0448-R

    authors: Fodor J,Kámán-Tóth E,Dankó T,Schwarczinger I,Bozsó Z,Pogány M

    更新日期:2018-01-01 00:00:00

  • Defense Gene Expression Analysis of Arabidopsis thaliana Parasitized by Orobanche ramosa.

    abstract::ABSTRACT The infection of Arabidopsis thaliana roots with the obligate parasite Orobanche ramosa represents a useful model for a study of the molecular events involved in the host plant response to a parasitic plant attack. To avoid analysis problems due to the subterranean development of O. ramosa, we developed two i...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2003.93.4.451

    authors: Dos Santos CV,Letousey P,Delavault P,Thalouarn P

    更新日期:2003-04-01 00:00:00

  • Identification and Characterization of Menadione and Benzethonium Chloride as Potential Treatments of Pierce's Disease of Grapevines.

    abstract::Xylella fastidiosa infects a wide range of plant hosts and causes Pierce's disease (PD) of grapevines. The type 1 multidrug resistance (MDR) efflux system is essential for pathogenicity and survival of bacterial pathogens in planta. X. fastidiosa, with a single MDR system, is significantly more vulnerable to inhibitio...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-07-18-0244-FI

    authors: Zhang S,Jain M,Fleites LA,Rayside PA,Gabriel DW

    更新日期:2019-02-01 00:00:00

  • Identification of a maize kernel stress-related protein and its effect on aflatoxin accumulation.

    abstract::ABSTRACT Aflatoxins are carcinogens produced mainly by Aspergillus flavus during infection of susceptible crops such as maize. Through proteomic comparisons of maize kernel embryo proteins of resistant and susceptible genotypes, several protein spots previously were found to be unique or upregulated in resistant embry...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2004.94.9.938

    authors: Chen ZY,Brown RL,Damann KE,Cleveland TE

    更新日期:2004-09-01 00:00:00

  • Improved sampling methods for real-time polymerase chain reaction diagnosis of citrus canker from field samples.

    abstract::ABSTRACT Citrus bacterial canker disease has been introduced at least three times into Florida in the last 15 years and, despite federal and state quarantine and eradication efforts, continues to spread in Florida. Accurate, fast, and reliable detection of the causal agent is of great importance. However, citrus bacte...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2004.94.1.61

    authors: Mavrodieva V,Levy L,Gabriel DW

    更新日期:2004-01-01 00:00:00

  • Genetic Control and Host Range of Avirulence Toward Brassica napus Cultivars Quinta and Jet Neuf in Leptosphaeria maculans.

    abstract::ABSTRACT Leptosphaeria maculans causes blackleg of oilseed rape. Gene-for-gene interactions between race PG3 and Brassica napus cv. Quinta were related to interaction between the fungal avirulence (Avr) gene AvrLm1 and the corresponding resistance gene Rlm1. AvrLm1 isolates were aviru-lent on cvs. Doublol, Vivol, Colu...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2001.91.1.70

    authors: Balesdent MH,Attard A,Ansan-Melayah D,Delourme R,Renard M,Rouxel T

    更新日期:2001-01-01 00:00:00

  • Anaerobic Soil Disinfestation Reduces Germination and Affects Colonization of Sclerotium rolfsii Sclerotia.

    abstract::Growth chamber and field studies were conducted with organic amendment mixtures of carbon (C) and nitrogen (N) at C:N ratios 10:1, 20:1, 30:1, and 40:1 and amendment rates of C at 2, 4, 6, and 8 mg/g of soil (C:N ratio 30:1) to evaluate anaerobic soil disinfestation (ASD) effects on germination and colonization of Scl...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-04-17-0152-R

    authors: Shrestha U,Dee ME,Ownley BH,Butler DM

    更新日期:2018-03-01 00:00:00

  • Interactions Between Fusarium verticillioides, Aspergillus flavus, and Insect Infestation in Four Maize Genotypes in Lowland Africa.

    abstract::ABSTRACT An experiment was designed to compare cycles of selection of four maize genotypes for ear- and grain-quality characteristics, interactions with Aspergillus flavus and Fusarium verticillioides infection, and insect ear infestation in two seasons. Mean infection levels by A. flavus and F. verticillioides were s...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2000.90.3.276

    authors: Cardwell KF,Kling JG,Maziya-Dixon B,Bosque-Pérez NA

    更新日期:2000-03-01 00:00:00

  • Limitations to photosynthesis in leaves of wheat plants infected by Pyricularia oryzae.

    abstract::Blast, caused by Pyricularia oryzae, has become an economically important disease in wheat in Brazil, but little effort has been devoted to understanding the wheat-P. oryzae interaction. This study was intended to determine the effects of P. oryzae infection on the photosynthetic process in wheat plants using a suscep...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-01-13-0024-R

    authors: Debona D,Rodrigues FÁ,Rios JA,Martins SC,Pereira LF,DaMatta FM

    更新日期:2014-01-01 00:00:00

  • White pine blister rust resistance in limber pine: evidence for a major gene.

    abstract::Limber pine (Pinus flexilis) is being threatened by the lethal disease white pine blister rust caused by the non-native pathogen Cronartium ribicola. The types and frequencies of genetic resistance to the rust will likely determine the potential success of restoration or proactive measures. These first extensive inocu...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-04-13-0092-R

    authors: Schoettle AW,Sniezko RA,Kegley A,Burns KS

    更新日期:2014-02-01 00:00:00

  • Ultrastructural and Cytochemical Investigation of Asymptomatic Infection by Pythium spp.

    abstract::ABSTRACT The influence exerted by Pythium group F (a minor pathogen ubiquitous in soilless cultures) and P. uncinulatum (a nonpathogenic species) colonization on tomato roots was investigated. In both interactions, infected roots did not exhibit obvious symptoms; however, major physiological changes occurred within th...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1998.88.3.234

    authors: Rey P,Benhamou N,Tirilly Y

    更新日期:1998-03-01 00:00:00

  • Evidence for Sexual Reproduction: Identification, Frequency, and Spatial Distribution of Venturia effusa (Pecan Scab) Mating Type Idiomorphs.

    abstract::Venturia effusa (syn. Fusicladium effusum), causal agent of pecan scab, is the most prevalent pathogen of pecan (Carya illinoinensis), causing severe yield losses in the southeastern United States. V. effusa is currently known only by its asexual (conidial) stage. However, the degree and distribution of genetic divers...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-07-17-0233-R

    authors: Young CA,Bock CH,Charlton ND,Mattupalli C,Krom N,Bowen JK,Templeton M,Plummer KM,Wood BW

    更新日期:2018-07-01 00:00:00

  • Salicylic Acid Suppresses Potato virus Y Isolate N:O-Induced Symptoms in Tobacco Plants.

    abstract::ABSTRACT The effects of salicylic acid (SA) and 1-aminocyclopropane-1-carboxylic acid (ACC) on the systemic development of symptoms induced by a severe isolate of Potato virus Y group N:O (PVY(N:O)) in tobacco were investigated. Upon inoculation, the systemic development of symptoms in tobacco plants could be divided ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-96-0255

    authors: Nie X

    更新日期:2006-03-01 00:00:00

  • Silicon-induced cell wall fortification of rice leaves: a possible cellular mechanism of enhanced host resistance to blast.

    abstract::ABSTRACT Locations of silicon accumulation in rice leaves and its possible association with resistance to rice blast were investigated by electron microscopy and X-ray microanalysis. A blast-susceptible cultivar, Jinmi, and a partially resistant cultivar, Hwaseong, were grown under a hydroponic culture system with mod...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2002.92.10.1095

    authors: Kim SG,Kim KW,Park EW,Choi D

    更新日期:2002-10-01 00:00:00

  • Population Genetic Structure and Cryptic Species of Plasmopara viticola in Australia.

    abstract::Downy mildew of grape caused by Plasmopara viticola is a global pathogen of economic importance to commercial viticulture. In contrast to populations in the northern hemisphere, few studies have investigated the population biology, genetic diversity, and origin of the pathogen in Australian production systems. DNA was...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-04-19-0146-R

    authors: Taylor AS,Knaus BJ,Grünwald NJ,Burgess T

    更新日期:2019-11-01 00:00:00

  • Specific Diagnosis of Two Root-Knot Nematodes, Meloidogyne chitwoodi and M. fallax, with Satellite DNA Probes.

    abstract::ABSTRACT Meloidogyne chitwoodi and M. fallax are serious pests of potato, and both species have been recently designated as quarantine organisms in the European Community and in Canada. The sympatric and less damaging species M. hapla is often found associated with both of them under temperate climates. Here, we descr...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1999.89.5.380

    authors: Castagnone-Sereno P,Leroy F,Bongiovanni M,Zijlstra C,Abad P

    更新日期:1999-05-01 00:00:00

  • Purification and Biological Characterization of Host-Specific SV-Toxins from Stemphylium vesicarium Causing Brown Spot of European Pear.

    abstract::ABSTRACT Culture filtrates of a pathogenic isolate (IT37) of Stemphylium vesicarium, causing brown spot of European pear, induced veinal necrosis only on pear leaves susceptible to the pathogen. Two host-specific toxins, SV-toxins I and II, were purified from culture filtrates of IT37 by successively using Amberlite X...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1999.89.10.947

    authors: Singh P,Bugiani R,Cavanni P,Nakajima H,Kodama M,Otani H,Kohmoto K

    更新日期:1999-10-01 00:00:00