Toxicological assessment of chemicals using Caenorhabditis elegans and optical oxygen respirometry.

Abstract:

:Oxygen consumption is indicative of an organism's metabolic state, whereby alterations in respiration rate can result from the presence of different stimuli. Here, we develop a novel approach based on quenched fluorescence oxygen sensing and respirometry method for toxicity screening assays using the nematode Caenorhabditis elegans. Previously, C. elegans was established as a useful model in soil and aquatic toxicology studies. For existing toxicology screening approaches with C. elegans, however, the endpoint is lethality. In addition, the assay time frame for the existing approaches is considerably longer than that for the approach described here. We present a sensitive, robust, high-throughput platform using standard laboratory equipment for toxicological studies by measuring respiration rate in C. elegans animals using a phosphorescent probe.

journal_name

Environ Toxicol Chem

authors

Schouest K,Zitova A,Spillane C,Papkovsky D

doi

10.1897/08-083.1

subject

Has Abstract

pub_date

2009-04-01 00:00:00

pages

791-9

issue

4

eissn

0730-7268

issn

1552-8618

pii

08-083

journal_volume

28

pub_type

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