Phytochelatin induction, cadmium accumulation, and algal sensitivity to free cadmium ion in Scenedesmus vacuolatus.

Abstract:

:Phytochelatins are small, intracellular, metal-binding polypeptides produced by algae on exposure to increased metal concentration in their environment. The aim of the present study was to examine the relationship between phytochelatin concentration, bioaccumulated metal, and sensitivity of algal growth on cadmium exposure. For that purpose, intracellular cadmium concentration and thiol (glutathione, gamma-glutamylcysteine [gammaGluCys], and phytochelatins [PCn]) content were determined in the freshwater green alga Scenedesmus vacuolatus exposed to growth-inhibitory and noninhibitory concentrations of free Cd2+ in the range from 10(-14) to 10(-7) M. The algal growth rate was optimal up to a free Cd2+ concentration of 10(-9) M and then decreased by 40% at higher concentrations. The intracellular cadmium content increased sharply from 0.22 to 746 amol/cell over this free Cd2+ range. At the lowest Cd2+ concentration (control), glutathione was the only detectable thiol (127 amol/cell). With increasing Cd2+, formation of gammaGluCys and phytochelatins from PC2 to PC6 were observed. The predominant oligomer was PC3, with 42 amol/cell at the highest Cd2+ concentration (10(-7) M). The ratio of the concentration of total thiol groups to intracellular cadmium was important for maintaining optimal growth. In contrast, thiol groups from phytochelatins were never measured in excess to intracellular cadmium content.

journal_name

Environ Toxicol Chem

authors

Le Faucheur S,Behra R,Sigg L

doi

10.1897/04-394r.1

subject

Has Abstract

pub_date

2005-07-01 00:00:00

pages

1731-7

issue

7

eissn

0730-7268

issn

1552-8618

journal_volume

24

pub_type

杂志文章
  • Accumulation and DNA damage in fathead minnows (Pimephales promelas) exposed to 2 brominated flame-retardant mixtures, Firemaster 550 and Firemaster BZ-54.

    abstract::Firemaster 550 and Firemaster BZ-54 are two brominated formulations that are in use as replacements for polybrominated diphenyl ether (PBDE) flame retardants. Two major components of these mixtures are 2,3,4,5-tetrabromo-ethylhexylbenzoate (TBB) and 2,3,4,5-tetrabromo-bis(2-ethylhexyl) phthalate (TBPH). Both have been...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.94

    authors: Bearr JS,Stapleton HM,Mitchelmore CL

    更新日期:2010-03-01 00:00:00

  • Uptake of polycyclic aromatic hydrocarbon compounds by the gills of the bivalve mollusk Elliptio complanata.

    abstract::Polycyclic aromatic hydrocarbons (PAHs) and herbicides are important contaminants of world water systems with effects on aquatic organisms. The uptake of naphthalene, anthracene, and chrysene by gills of the bivalve mollusk Elliptio complanata was determined. Additionally, the effects of paraquat, atrazine, alachlor, ...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:

    authors: Birdsall K,Kukor JJ,Cheney MA

    更新日期:2001-02-01 00:00:00

  • Aquatic photochemistry of chlorinated triclosan derivatives: potential source of polychlorodibenzo-p-dioxins.

    abstract::Triclosan (TCS; 5-chloro-2-(2,4-dichlorophenoxy)phenol), a common antimicrobial agent, may react with residual chlorine in tap water during transport to wastewater treatment plants or during chlorine disinfection of wastewater, generating chlorinated TCS derivatives (CTDs): 4,5-dichloro-2-(2,4-dichlorophenoxy)phenol (...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/08-490.1

    authors: Buth JM,Grandbois M,Vikesland PJ,McNeill K,Arnold WA

    更新日期:2009-12-01 00:00:00

  • Simultaneous exposure to chronic hypoxia and dissolved polycyclic aromatic hydrocarbons results in reduced egg production and larval survival in the sheepshead minnow (Cyprinodon variegatus).

    abstract::Estuarine fish in the northern Gulf of Mexico are exposed annually to hypoxic conditions. In addition to hypoxia, fish located throughout the northern Gulf of Mexico were potentially exposed to oil released during the Deepwater Horizon incident. Therefore, the interaction between oil exposure and hypoxia is worth inve...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.3207

    authors: Hedgpeth BM,Griffitt RJ

    更新日期:2016-03-01 00:00:00

  • Development of whole-sediment toxicity identification and evaluation (TIE) techniques for two Australian freshwater species: Chironomus tepperi and Austrochiltonia subtenuis.

    abstract::Most of the public literature and available guidance documents on the conduct of freshwater whole-sediment toxicity identification and evaluations (TIEs) detail the use of test organisms and amending agents that are readily available in North America. These commonly used test organisms and the supported amending agent...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.3787

    authors: Mehler WT,Keough MJ,Pettigrove V

    更新日期:2017-09-01 00:00:00

  • An approach for calculating a confidence interval from a single aquatic sample for monitoring hydrophobic organic contaminants.

    abstract::The use of passive sampling devices (PSDs) for monitoring hydrophobic organic contaminants in aquatic environments can entail logistical constraints that often limit a comprehensive statistical sampling plan, thus resulting in a restricted number of samples. The present study demonstrates an approach for using the res...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.2014

    authors: Matzke MM,Allan SE,Anderson KA,Waters KM

    更新日期:2012-12-01 00:00:00

  • Validation of a new standardized test method for the freshwater amphipod Hyalella azteca: Determining the chronic effects of silver in sediment.

    abstract::Environment Canada has developed a new 42-d sediment toxicity test method that includes a reproduction test endpoint with the freshwater amphipod Hyalella azteca. Because of concerns that existing standard methodologies, whereby adults are transferred to a water-only exposure before release of their first brood at day...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.3453

    authors: Taylor LN,Novak L,Rendas M,Antunes PM,Scroggins RP

    更新日期:2016-10-01 00:00:00

  • Responses of microbial community from northern Gulf of Mexico sandy sediments following exposure to Deepwater Horizon crude oil.

    abstract::In the present study, microbial community responses to exposure to unweathered Macondo Well crude oil and conventional diesel in a sandy beach environment were determined. Biodegradation was assessed in mesocosm experiments with differing fuel amounts (2,000 and 4,000 mg/kg) and with or without inorganic nutrient amen...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.1770

    authors: Horel A,Mortazavi B,Sobecky PA

    更新日期:2012-05-01 00:00:00

  • Biodegradability relationships among propylene glycol substances in the Organization for Economic Cooperation and Development ready- and seawater biodegradability tests.

    abstract::Eight propylene glycol substances, ranging from 1,2-propanediol to a poly(propylene glycol) (PPG) having number-average molecular weight (M(n)) of 2,700 (i.e., PPG 2700), were evaluated in the Organization for Economic Cooperation and Development (OECD) ready- and seawater biodegradability tests. Uniformity in test pa...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/06-327r.1

    authors: West RJ,Davis JW,Pottenger LH,Banton MI,Graham C

    更新日期:2007-05-01 00:00:00

  • Detecting nanoparticulate silver using single-particle inductively coupled plasma-mass spectrometry.

    abstract::The environmental prevalence of engineered nanomaterials, particularly nanoparticulate silver (AgNP), is expected to increase substantially. The ubiquitous use of commercial products containing AgNP may result in their release to the environment, and the potential for ecological effects is unknown. Detecting engineere...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.719

    authors: Mitrano DM,Lesher EK,Bednar A,Monserud J,Higgins CP,Ranville JF

    更新日期:2012-01-01 00:00:00

  • Insecticide-induced changes in amphibian brains: How sublethal concentrations of chlorpyrifos directly affect neurodevelopment.

    abstract::Widespread use of pesticides often contaminates natural habitats, exposing nontarget organisms to pesticides that were designed to control pest populations. Even low levels of pesticides can affect aquatic communities both directly and indirectly. Previous work has shown that trace amounts of the pesticide chlorpyrifo...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.4240

    authors: McClelland SJ,Bendis RJ,Relyea RA,Woodley SK

    更新日期:2018-10-01 00:00:00

  • Intra- and interlaboratory calibration of the DR CALUX bioassay for the analysis of dioxins and dioxin-like chemicals in sediments.

    abstract::In the Fourth National Policy Document on Water Management in The Netherlands, it is defined that in 2003, in addition to the assessment of chemical substances, special guidelines for the assessment of dredged material should be recorded. The assessment of dredged material is based on integrated chemical and biologica...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/03-542.1

    authors: Besselink HT,Schipper C,Klamer H,Leonards P,Verhaar H,Felzel E,Murk AJ,Thain J,Hosoe K,Schoeters G,Legler J,Brouwer B

    更新日期:2004-12-01 00:00:00

  • Sediments are major sinks of steroidal estrogens in two United Kingdom rivers.

    abstract::The occurrence of intersex fish in a number of European rivers has been attributed to exposure to estrogenic chemicals present in sewage treatment work (STW) effluents. To further understand the environmental fate of these contaminants, the estrogenic activity of effluents, water, and sediments were investigated both ...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/03-41

    authors: Peck M,Gibson RW,Kortenkamp A,Hill EM

    更新日期:2004-04-01 00:00:00

  • Absorption of p,p'-dichlorodiphenyldichloroethylene and dieldrin in largemouth bass from a 60-d slow-release pellet and detection using a novel enzyme-linked immunosorbent assay method for blood plasma.

    abstract::This work describes the uptake of two organochlorine pesticides from slow-release pellets by largemouth bass and the utility of a blood plasma enzyme-linked immunosorbent assay (ELISA) method for exposure verification. We measured blood and tissue levels by gas chromatography/mass spectrometry and by a novel ELISA met...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/04-492r.1

    authors: Muller JK,Sepúlveda MS,Borgert CJ,Gross TS

    更新日期:2005-08-01 00:00:00

  • Measurement of triclosan in wastewater treatment systems.

    abstract::The objective of this study was to investigate the fate and removal of triclosan (TCS; 5-chloro-2-[2,4-dichloro-phenoxy]-phenol), an antimicrobial agent used in a variety of household and personal-care products, in wastewater treatment systems. This objective was accomplished by monitoring the environmental concentrat...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:

    authors: McAvoy DC,Schatowitz B,Jacob M,Hauk A,Eckhoff WS

    更新日期:2002-07-01 00:00:00

  • Ecotoxicological assessment of glyphosate-based herbicides: Effects on different organisms.

    abstract::Glyphosate-based herbicides are the most commonly used worldwide because they are effective and relatively nontoxic to nontarget species. Unlimited and uncontrolled use of such pesticides can have serious consequences for human health and ecological balance. The present study evaluated the acute toxicity and genotoxic...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.3580

    authors: de Brito Rodrigues L,de Oliveira R,Abe FR,Brito LB,Moura DS,Valadares MC,Grisolia CK,de Oliveira DP,de Oliveira GAR

    更新日期:2017-07-01 00:00:00

  • Marine sediment toxicity identification evaluation methods for the anionic metals arsenic and chromium.

    abstract::Marine sediments accumulate a variety of contaminants and, in some cases, demonstrate toxicity because of this contamination. Toxicity identification evaluation (TIE) methods provide tools for identifying the toxic chemicals causing sediment toxicity. Currently, whole-sediment TIE methods are not available for anionic...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/06-014r.1

    authors: Burgess RM,Perron MM,Cantwell MG,Ho KT,Pelletier MC,Serbst JR,Ryba SA

    更新日期:2007-01-01 00:00:00

  • Tracking multiple modes of endocrine activity in Australia's largest inland sewage treatment plant and effluent- receiving environment using a panel of in vitro bioassays.

    abstract::Estrogenicity of sewage effluents, and related ecotoxicological effects in effluent-receiving environments, have been widely reported over the last 2 decades. However, relatively little attention has been given to other endocrine pathways that may be similarly disrupted by a growing list of contaminants of concern. Fu...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.3051

    authors: Roberts J,Bain PA,Kumar A,Hepplewhite C,Ellis DJ,Christy AG,Beavis SG

    更新日期:2015-10-01 00:00:00

  • Use of the Australian crimson-spotted rainbowfish (Melanotaenia fluviatilis) as a model test species for investigating the effects of endocrine disruptors.

    abstract::Few studies have investigated the potential reproductive effects of toxicants on Australian freshwater fish species. The present study uses the Australian rainbowfish (Melanotaenia fluviatilis) as a model for testing the potential effects of 17beta-estradiol. Groups of reproductively active rainbowfish were exposed to...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/06-603R.1

    authors: Pollino CA,Georgiades E,Holdway DA

    更新日期:2007-10-01 00:00:00

  • Molecular simulation of a model of dissolved organic matter.

    abstract::A series of atomistic simulations was performed to assess the ability of the Schulten dissolved organic matter (DOM) molecule, a well-established model humic molecule, to reproduce the physical and chemical behavior of natural humic substances. The unhydrated DOM molecule had a bulk density value appropriate to humic ...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/04-567r.1

    authors: Sutton R,Sposito G,Diallo MS,Schulten HR

    更新日期:2005-08-01 00:00:00

  • Environmental fractionation of PCBs and PBDEs during particle transport as recorded by sediments in coastal waters.

    abstract::The Strait of Georgia (British Columbia, Canada) is a hydrologically complex inland sea with a rich abundance and diversity of species of aquatic life. Marine sediments, as both a sink for hydrophobic contaminants and a potential source for aquatic food webs, were collected from 41 sites throughout the 6,900-km(2) Str...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.542

    authors: Grant PB,Johannessen SC,Macdonald RW,Yunker MB,Sanborn M,Dangerfield N,Wright C,Ross PS

    更新日期:2011-07-01 00:00:00

  • Cytotoxicity and aryl hydrocarbon receptor-mediated activity of n-heterocyclic polycyclic aromatic hydrocarbons: structure-activity relationships.

    abstract::Toxic effects of many persistent organic pollutants (e.g., polychlorinated biphenyls or polychlorinated dibenzo-p-dioxins and furans) are mediated via the aryl hydrocarbon receptor (AhR). Although polycyclic aromatic hydrocarbons (PAHs) and their derivatives also activate AhR, their toxic effects remain to be fully el...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/05-388r.1

    authors: Sovadinová I,Bláha L,Janosek J,Hilscherová K,Giesy JP,Jones PD,Holoubek I

    更新日期:2006-05-01 00:00:00

  • Interference of contaminated sediment extracts and environmental pollutants with retinoid signaling.

    abstract::Retinoids are known to regulate important processes such as differentiation, development, and embryogenesis. Some effects, such as malformations in frogs or changes in metabolism of birds, could be related to disruption of the retinoid signaling pathway by exposure to organic contaminants. A new reporter gene assay ha...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/06-513r.1

    authors: Novák J,Benísek M,Pacherník J,Janosek J,Sídlová T,Kiviranta H,Verta M,Giesy JP,Bláha L,Hilscherová K

    更新日期:2007-08-01 00:00:00

  • Reproductive effects and bioaccumulation of chlordane in Daphnia magna.

    abstract::Acute and chronic toxicity of high-grade chlordane (98%) and bioaccumulation were investigated in Daphnia magna at water soluble concentrations obtained without cosolvent. The measured effective concentrations immobilizing 50% of the microcrustacea (95% confidence interval) were 22.6 (19.7-26.1) microg/L at 24 h and 1...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/08-564.1

    authors: Manar R,Bessi H,Vasseur P

    更新日期:2009-10-01 00:00:00

  • Biodegradation of plasticizers by Rhodotorula rubra.

    abstract::The degradation of plasticizers by the yeast Rhodotorula rubra J-96-1 (American Type Culture Collection 9449) in the presence of glucose was studied. The plasticizers included the commonly used bis-2-ethylhexyl adipate (B(EH)A), dioctyl phthalate (DOP), and dioctyl terephthalate (DOTP), and the less commonly used dipr...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:

    authors: Gartshore J,Cooper DG,Nicell JA

    更新日期:2003-06-01 00:00:00

  • Metabolism of uniconazole-P in water-sediment systems under illumination.

    abstract::Aerobic soil metabolism of uniconazole-P ([S]-E-1-[4-chlorophenyl]-4,4-dimethyl-2-[1,2,4-triazole-1-yl]-penten-3-ol) and the effect of illumination on metabolic profiles were studied in the water-sediment system when spiked to water. Uniconazole-P was gradually partitioned to the sediment with an aquatic half-life of ...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/05-116ra.1

    authors: Kodaka R,Sugano T,Katagi T

    更新日期:2006-02-01 00:00:00

  • The theory underlying dose-response models influences predictions for intermittent exposures.

    abstract::Prediction of pulsed or intermittent exposure effects on populations is emerging as an important issue in ecotoxicology. However, the underlying theory of the dose-response models has not been tested rigorously enough to provide a true understanding for predicting the effects of pulsed exposures. Since its introductio...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/06-398r.1

    authors: Zhao Y,Newman MC

    更新日期:2007-03-01 00:00:00

  • Environmental cadmium induces histopathological changes in kidneys of roe deer.

    abstract::Kidney samples of 224 roe deer (113 males, 111 females) aged three months to approximately nine years were collected in eastern Austria. Cadmium contents were examined and histological examinations were performed considering 11 different pathomorphological parameters. Cadmium burden was relatively high (range: 0.010-2...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:

    authors: Beiglböck C,Steineck T,Tataruch F,Ruf T

    更新日期:2002-09-01 00:00:00

  • A short life-cycle test with the epibenthic copepod Nitocra spinipes for sediment toxicity assessment.

    abstract::A new short life-cycle test methodology is presented to evaluate sublethal effects of contaminated sediments to the harpacticoid copepod species Nitocra spinipes. The method combines a 4-d survival-gravidity test with a 7-d development test. For water-only Cu exposures, the sensitivity of the development test endpoint...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.513

    authors: Perez-Landa V,Simpson SL

    更新日期:2011-06-01 00:00:00

  • High sensitivity of cyanobacterium Microcystis aeruginosa to copper and the prediction of copper toxicity.

    abstract::Microcystis aeruginosa is a dominant cyanobacterium commonly found in Chinese freshwater ecosystems during phytoplankton blooms, and copper sulfate is one of the most frequently used algicides for controlling the nuisance blooms. In this study, we examined the effects of varied water chemistry (dissolved organic matte...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.266

    authors: Zeng J,Yang L,Wang WX

    更新日期:2010-10-01 00:00:00