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Recovery of malondialdehyde in urine as a 2,4-dinitrophenylhydrazine derivative analyzed with high-performance liquid chromatography.

Abstract：

:Malondialdehyde (MDA) in urine was measured as a 2,4-dinitrophenylhydrazine (DNPH) derivative using high-performance liquid chromatography (HPLC) for the analysis. MDA standard coeluted with a peak obtained from rat urine after i.p. administration of MDA standard. This peak was also the only peak containing 14C after injection of a [14C]MDA standard, and was shown by mass spectrometry to contain 1-(2,4-dinitrophenyl)pyrazole, the derivative formed when MDA is treated with DNPH. Depending on the amount given (0.3-5.5 mumol), the recovery (after 24 h sampling period) in urine was 0.7-2.6%. This apparent non-linear kinetics may relate to several factors, such as dose-dependent metabolism. However, the peak urinary concentration approached the expected plasma concentration and reproducible recovery data were obtained, suggesting that MDA was passively excreted in a reasonably stable form. These data indicate that monitoring MDA excretion in urine can give useful information about lipid peroxidation in vivo.

journal_name

Chem Biol Interact

journal_title

Chemico-biological interactions

authors

Ekström T,Garberg P,Egestad B,Högberg J

doi

10.1016/0009-2797(88)90070-1

subject

Has Abstract

pub_date

1988-01-01 00:00:00

pages

177-87

issue

3-4

eissn

0009-2797

issn

1872-7786

pii

0009-2797(88)90070-1

journal_volume

66

pub_type

杂志文章

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