Abstract:
BACKGROUND/AIM:γδ T cells mediate cytotoxicity against prostate cancer (PCa) cells in vitro; however, the clinical efficacy of γδ T cell-targeted immunotherapy for recurrent and metastatic PCa is unsatisfactory. We hypothesized that the resistance of recurrent and metastatic PCa to γδ T cells is related to the presence of prostate cancer stem cells (PCSCs), and we examined their relationship. MATERIALS AND METHODS:PCa spheres (prostaspheres) were generated from five PCa cell lines, and their susceptibility to cytotoxicity by γδ T cells was investigated. Expression of stemness-related markers was evaluated by qRT-PCR. RESULTS:Prostasphere-derived cancer cells were resistant to lysis by γδ T cells and expressed higher levels of several stemness markers, including CD133, NANOG, SOX2, and OCT4, than the parental PCa cell lines. CONCLUSION:Ex vivo-expanded γδ T cells are not effective against PCSCs.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Miyashita M,Tomogane M,Nakamura Y,Shimizu T,Fujihara A,Ukimura O,Ashihara Edoi
10.21873/anticanres.14559subject
Has Abstractpub_date
2020-10-01 00:00:00pages
5481-5487issue
10eissn
0250-7005issn
1791-7530pii
40/10/5481journal_volume
40pub_type
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