Abstract：
BACKGROUND/AIM:Bladder cancer (BLCA, urothelial bladder cancer) is one of the most common malignancies with increasing incidence and mortality worldwide. Poor diagnosis and the limitation of treatment is still an unmet need in clinical practice. γδ T-Cells have been paid increasing attention because of their potent cytotoxicity against tumors. Herein, we investigated the cytolytic effect of γδ T-cells in combination with the chemotherapeutic drug, carboplatin, against BLCA cells. MATERIALS AND METHODS:The standard protocol for the induction and expansion of peripheral blood mononuclear cell-derived γδ T-cells was a zoledronic acid/interleukin-2-based medium system for 2 weeks. The cytotoxicity of γδ T-cells with and without carboplatin against BLCA cells was examined. RESULTS:After incubation, T-cell receptor-positive γδ T-cells showed a natural killer cell-like phenotypic characteristic and dose-dependently increased cytotoxicity against BLCA cells. Interestingly, we found that in advanced BLCA cells, which were more resistant to carboplatin, the cell viability was significantly (p<0.05) reduced in the presence of γδ T-cells. CONCLUSION:Our findings showed that γδ T-cell therapy has potent benefit in cancer treatment.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Pan Y,Chiu YH,Chiu SC,Cho DY,Lee LM,Wen YC,Whang-Peng J,Hsiao CH,Shih PHdoi
10.21873/anticanres.14525subject
Has Abstractpub_date
2020-09-01 00:00:00pages
5221-5227issue
9eissn
0250-7005issn
1791-7530pii
40/9/5221journal_volume
40pub_type
杂志文章abstract：:Adrenomedullin (AM) is a hypotensive polypeptide that has been shown to stimulate cyclic AMP and intracellular free Ca2+ agents that are known to induce expression of proto-oncogenes, in various cell types. Transforming growth factor-beta 1 (TGF-beta1) is a multifunctional polypeptide that regulates proliferation, dif...
journal_title：Anticancer research
pub_type： 杂志文章
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更新日期：2002-05-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
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journal_title：Anticancer research
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journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：2000-03-01 00:00:00
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journal_title：Anticancer research
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doi：
更新日期：2002-01-01 00:00:00
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pub_type： 杂志文章,多中心研究
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更新日期：2007-07-01 00:00:00
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journal_title：Anticancer research
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更新日期：1996-05-01 00:00:00
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