Abstract:
:The aim of this study was to investigate the correlation between contractile function recovery and changes of acetylcholine receptors (AChR) in a transferred muscle flap following reinnervation. Orthotopic transfer of the gracilis muscle flap with repair of its nerve was performed bilaterally in 48 rats. The rats were randomly divided into six experimental groups based on the time intervals for assessments (1, 4, 5, 10, 20, and 30 weeks). Sixteen gracilis muscle samples from eight rats without surgery were used as the controls. In each group, muscle contractile force and weight were measured (n = 16). The AChR numbers (n = 8) and subunits (epsilon and gamma) mRNA (n = 8) were examined using [(125)I]-alpha-bungarotoxin and fluorescent quantitative-PCR. The results showed the AChR numbers in the muscle flap increased from 4 to 20 weeks after reinnervation and correlated with recovery of the tetanic contraction force. However, correlation between the increase of AChR number with the specific tension (peak contractile force normalized to wet muscle weight) was only found from 4 to 10 weeks postoperatively. The expression of gamma-subunit mRNA increased at the early period after flap transfer and then decreased rapidly, whereas the epsilon-subunit mRNA recovered gradually since fourth week postoperatively. A small amount of gamma-subunit mRNA could still be detected at 30 weeks after surgery. In conclusion, following reinnervation of the transferred muscle flap, the contractile functional recovery is partially correlated to increase of the AChRepsilon. Our findings may provide evidence for further study of improving muscle function in functional reconstruction by targeting the AChR.
journal_name
Microsurgeryjournal_title
Microsurgeryauthors
Zhang Y,Liu A,Zhang W,Jiang H,Cai Zdoi
10.1002/micr.20727subject
Has Abstractpub_date
2010-05-01 00:00:00pages
307-13issue
4eissn
0738-1085issn
1098-2752journal_volume
30pub_type
杂志文章相关文献
MICROSURGERY文献大全abstract:INTRODUCTION:The basic idea of video-microsurgery is the improvement of ergonomic conditions in microsurgical procedures by replacing the bulky operating microscope with a compact videosystem. OBJECTIVE:To specify optical requirements on a videosystem for microsurgical intracranial procedures in neurosurgery. METHODS...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.20915
更新日期:2011-10-01 00:00:00
abstract::The iliac crest is reevaluated with respect to the rapid evolution of vascularized mandibular reconstruction. With more flaps to choose from, indications for the crest have become more specific-playing to the strengths of this particular transfer. Ideally, defects should exclude soft tissue and occupy lateral segments...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.1920150406
更新日期:1994-01-01 00:00:00
abstract::Nerve injuries to the upper and inferior limbs represent a common event due both to home and working accidents. Minor traumas can lead to severe disabilities if a wrong treatment is carried out. From 1997 to 2005, 920 patients were observed with a total of 1,200 major or minor nerves injuries operated. In 852 cases ac...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.20382
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Identification of lymphatic vessels for lymphaticovenular anastomosis (LVA), which is an effective surgical treatment for obstructive lymphedema, is important. Indocyanine green (ICG) lymphography is useful for that purpose, but is not common in many institutions. Although ultrasound is a very common modalit...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.22414
更新日期:2016-07-01 00:00:00
abstract::The single-anastomosis heart-lung transplantation method in the rat developed by Lee and associates has been examined and has proven to be simple and reproducible. Technical procedures are discussed emphasizing those procedures that require special caution to ensure successful graft function. The donor and recipient r...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.1920080411
更新日期:1987-01-01 00:00:00
abstract::In microvascular reconstructive surgery the patency of the recipient vessels is the key to successful outcome. In head and neck surgery there is often a lack of adequate recipient vessels as a result of chemoradiation therapy and ablative surgery. To overcome this it is crucial to identify vessels of adequate length a...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.20680
更新日期:2009-01-01 00:00:00
abstract::We used the rat medial gastrocnemius free flap, based on a pedicle of the femoral artery and vein, in order to test the tolerance of skeletal muscle to cold ischemia-reperfusion (IR) injury, and to determine whether tolerance can be enhanced by pre-ischemic perfusion with tissue/organ preservation solutions. Muscle fl...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/1098-2752(2000)20:7<343::aid-micr6>3.0.co;
更新日期:2000-01-01 00:00:00
abstract::The histology of free microvascular muscle flaps in 19 patients was studied prospectively. Biopsies were taken during operation, and after 2 and 6 weeks, as well as 3, 6, and 9 months, postoperatively, fixed and stained using the van Gieson method. Fiber diameters were analyzed morphometrically and fiber types defined...
journal_title:Microsurgery
pub_type: 临床试验,杂志文章
doi:10.1002/(sici)1098-2752(1998)18:2<137::aid-micr13>
更新日期:1998-01-01 00:00:00
abstract::Severe injuries at foot and ankle level with loss of soft tissues and bone are often treated by means of amputation. The transfer of composite free flaps from various donor sites may provide anatomical reconstruction of the foot and ankle and function. Ten patients who sustained severe combined tissue injuries of the ...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.20863
更新日期:2011-03-01 00:00:00
abstract::Consecutive or simultaneous contralateral breast reduction is a frequent request from patients undergoing unilateral breast reconstruction. Both procedures can be combined using otherwise discarded tissue as a split breast graft for reconstruction of the contralateral side. There have been few reports on the use of pe...
journal_title:Microsurgery
pub_type:
doi:10.1002/micr.30654
更新日期:2020-09-19 00:00:00
abstract::Vascular microanastomosis of vessels that presents marked discrepancy in size can be performed without difficulty by making a longitudinal incision of the end of the smallest vessel, forming a kind of "spatula," and joined to the vessel of greater diameter. ...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.1920060111
更新日期:1985-01-01 00:00:00
abstract::This experimental study investigates the feasibility of pretreating the abdominal skin of a rat with subdermal injections of adenovirus encoding angiopoietin-1 in order to improve postoperative survival of the skin flap. An epigastric skin flap was used as the model in this study. Rats received subdermal injections of...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.10140
更新日期:2003-01-01 00:00:00
abstract::The effect of isovolemic hemodilution using dextran-60 on arterial blood flow in the rabbit ear was investigated. The animals were anesthetized and an electromagnetic flow probe was applied around the central artery of the ear. Isovolemic hemodilution was performed during a 15 min exchange period. Two degrees of hemod...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.1920110106
更新日期:1990-01-01 00:00:00
abstract::We have treated three patients with avascular osteonecrosis using vascularized scapular bone graft. To predict blood perfusion in both the diseased femoral head and the transferred bone, all the hips were followed up using dynamic magnetic resonance (MR) scans, performed 1 and 7 months after surgery. In the present ca...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.1920161004
更新日期:1995-01-01 00:00:00
abstract::Replantation of amputated rat feet utilizing an efferent arteriovenous shunt constructed between the distal posterior tibial artery and the proximal posterior tibial vein, in the absence of all other venous drainage, provides an alternative pathway to the normal venous drainage in a replanted rat foot. However, this s...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.1920130112
更新日期:1992-01-01 00:00:00
abstract::The authors report their experience of the application of microsurgical techniques in total and subtotal thyroidectomy operations from January 1984-January 1997. Four hundred and eighty-six patients with thyroid diseases were operated upon with both traditional and microsurgical techniques. The comparison of results s...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/(sici)1098-2752(1998)18:8<460::aid-micr6>3
更新日期:1998-01-01 00:00:00
abstract::The authors present two cases of complete median nerve paralysis due to entrapment after posterior dislocation of the elbow. Previous literature on the subject matter is reviewed, and management and therapeutic indication of these lesions are discussed. ...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.1920040403
更新日期:1983-01-01 00:00:00
abstract::A 4-year-old girl who sustained the hemiplegic cerebral palsy and subsequent spasticity in the left upper extremity underwent the C7 nerve root rhizotomy and the contralateral C7 nerve root transfer to the ipsilateral middle trunk of brachial plexus through an interpositional sural nerve graft. In a 2-year follow-up, ...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.20877
更新日期:2011-07-01 00:00:00
abstract::Femoral nerve lesions are uncommon, but very distressing at the functional level because of the absence of knee locking mechanism by the quadriceps muscle. We propose here a new neurotization procedure of obturator nerve motor branches to the motor portion of the femoral nerve in the thigh. This study was conducted on...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.22012
更新日期:2012-09-01 00:00:00
abstract:BACKGROUND:Reconstruction of long and deep sternal defects has been challenging. The pectoralis major can be used in the conventional turnover method that requires the internal thoracic vessel. We developed a new turnover pectoralis major flap based on thoracoacromial vessels. The purpose of this report is to present r...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.30509
更新日期:2020-02-01 00:00:00
abstract::Both hypotension and hypertension aggravate the damage of reperfusion injury after reconstructive microsurgery. The purpose of this study is to establish a theoretical guide for postoperative blood pressure control in optimizing the cutaneous perfusion and flap survival. Systemic arterial pressure was altered by the i...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.20627
更新日期:2009-01-01 00:00:00
abstract::The secondary lymphedema of the upper limb (post-mastectomy lymphedema) has an incidence, in patients who underwent axillary lymphadenectomy for breast cancer, between 5 to 25%, up to 40% after radiotherapic treatment. We studied 50 patients treated for breast cancer. The patients were divided in two groups of 25 each...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.20215
更新日期:2006-01-01 00:00:00
abstract:INTRODUCTION:The anterolateral thigh (ALT) flap is considered a workhorse reconstructive option; however, it is encumbered by its bulkiness that can result in poor final outcome and need for revision surgery. The aim of the present study was to compare the standard cutaneous ALT free flap and sandwich fascial ALT (SALT...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.30515
更新日期:2020-05-01 00:00:00
abstract::The authors present a new technique of end-to-side microvascular anastomosis in a rat carotid artery model, employing a milliwatt CO2 laser. Both carotid arteries were isolated and approximated in an end-to-side fashion by the placement of four 10-0 nylon stay sutures. The milliwatt CO2 laser was used to effect vessel...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.1920070206
更新日期:1986-01-01 00:00:00
abstract::Several technical advances over the years have allowed microsurgery to be successful. However, thrombosis, particularly at the anastomosis site, remains a complication of microvascular surgery. Various preventive measures, including drugs, have been used to improve microvascular surgical anastomotic patency rates. The...
journal_title:Microsurgery
pub_type: 杂志文章,评审
doi:10.1002/micr.1920150905
更新日期:1994-01-01 00:00:00
abstract::On the dorsum of adult Sprague-Dawley rats, 3 x 7 cm pedicle flaps were raised and a temporary arteriovenous shunt (AVS) from anastomosis of the tail artery and vein was placed beneath. Fourteen, 21 and 28 days later, the pedicle flaps were converted to island flaps leaving only the AVS attachment. The degree of subse...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.1920150115
更新日期:1994-01-01 00:00:00
abstract:OBJECTIVE:Mandibular reconstruction with a mandibular reconstruction plate (MRP) and free flap transfer can involve serious plate-related complications. The aim of our study is to present our new "no-touch-technique" which keeps an MRP not contaminated to saliva during the entire operation for the reduction of plate-re...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.22403
更新日期:2016-02-01 00:00:00
abstract:PURPOSE:Replantation of amputations separated by more than four digits is challenging microsurgery because of the technical demands and long operation time. The purpose of this report was to present the clinical and functional outcomes in patients with amputations of four or five digits using a simultaneous replantatio...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.30009
更新日期:2016-03-01 00:00:00
abstract::Despite the high success rate of free-tissue transfer, thrombosis still complicates 5-30% of cases. Meticoulous technique, careful vessel selection, and pharmacological prophylaxis are not always enough to avoid thrombosis. Early diagnosis and reintervention provide the only way to salvage a thrombosed free flap, in c...
journal_title:Microsurgery
pub_type: 杂志文章
doi:10.1002/micr.20147
更新日期:2005-01-01 00:00:00
abstract:BACKGROUND:Wound dehiscence, infection, and necrosis of tendon and overlying skin are severe complications after open repairs of Achilles tendon. A simultaneous reconstruction should be provided in a single stage operation. We evaluated the outcomes of one of the possible options: the radial forearm free flap with Flex...
journal_title:Microsurgery
pub_type: 临床试验,杂志文章
doi:10.1002/micr.22481
更新日期:2015-11-01 00:00:00