Abstract:
:Chiral separation of 19 pairs of cis-beta-lactam (BL) stereoisomers of pharmacological importance was examined by capillary electrophoresis using cyclodextrin (CD) derivatives. In order to select the most effective conditions separating the highest number of stereoisomers of BLs, single carboxymethyl alpha-, beta- and gamma-, as well as sulfobutyl beta-CD derivatives were applied. Additionally, carboxymethyl and sulfobutyl beta-CD derivatives complemented with neutral beta-CD derivatives as dual CD systems were tested. Both the composition and concentration of applied selectors and the pH of background electrolyte were selected. In single systems the structural characteristics of BLs and the complex forming affinity were correlated. Most BLs provided optimal complexation with beta-CD derivatives. In conclusion, the efficiency of combining sulfobutyl-beta-CD and permethylated beta-CD was superior to other single and dual CD systems applied. This method successfully separated each pair of stereoisomers investigated.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Németh K,Varga E,Iványi R,Szemán J,Visy J,Jicsinszky L,Szente L,Forró E,Fülöp F,Péter A,Simonyi Mdoi
10.1016/j.jpba.2010.04.026subject
Has Abstractpub_date
2010-11-02 00:00:00pages
382-8issue
3eissn
0731-7085issn
1873-264Xpii
S0731-7085(10)00267-0journal_volume
53pub_type
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