Analysis of nerve and neuropeptide patterns in vacuum-assisted closure-treated diabetic murine wounds.

Abstract:

BACKGROUND:Reestablishment of the peripheral nervous system occurs in parallel with wound healing. With accelerated wound healing seen with the vacuum-assisted closure device, the authors studied its effects on nerve fiber regeneration, nerve sprouting, and the stimulation of neuropeptides and neurotrophins. METHODS:A vacuum-assisted closure device was applied to a full-thickness diabetic mouse wound using continuous or cyclical modes and compared with foam dressing or occlusive dressing controls, using 10 mice per group. Nerve fibers, substance P, calcitonin gene-related peptide, and nerve growth factor were analyzed using two-dimensional immunohistochemistry and real-time reverse-transcriptase polymerase chain reaction. RESULTS:A significant increase in dermal and epidermal nerve fiber densities and in substance P, calcitonin gene-related peptide, and nerve growth factor expression was seen in vacuum-assisted closure-treated wounds. Cyclical treatment mode correlated with the largest increase in granulation tissue production, wound surface microdeformations, and a slightly faster wound closure rate. CONCLUSIONS:This study suggests that vacuum-assisted closure therapy can modulate nerve fiber and neuropeptide production in the wound. Optimized kinetics of vacuum-assisted closure application may provide an opportunity for clinicians to further improve wound healing in denervated wounds such as pressure sores and diabetic foot ulcerations.

journal_name

Plast Reconstr Surg

authors

Younan G,Ogawa R,Ramirez M,Helm D,Dastouri P,Orgill DP

doi

10.1097/PRS.0b013e3181da86d0

subject

Has Abstract

pub_date

2010-07-01 00:00:00

pages

87-96

issue

1

eissn

0032-1052

issn

1529-4242

pii

00006534-201007000-00011

journal_volume

126

pub_type

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