Abstract：
:In the last 15 years, single-cell technologies have become robust and indispensable tools to investigate cell heterogeneity. Beyond transcriptomic, genomic and epigenome analyses, technologies are constantly evolving, in particular toward multi-omics, where analyses of different source materials from a single cell are combined, and spatial transcriptomics, where resolution of cellular heterogeneity can be detected in situ. While some of these techniques are still being optimized, single-cell RNAseq has commonly been used because the examination of transcriptomes allows characterization of cell identity and, therefore, unravel previously uncharacterized diversity within cell populations. Most endocrine organs have now been investigated using this technique, and this has given new insights into organ embryonic development, characterization of rare cell types, and disease mechanisms. Here, we highlight recent studies, particularly on the hypothalamus and pituitary, and examine recent findings on the pancreas and reproductive organs where many single-cell experiments have been performed.
journal_name
J Mol Endocrinoljournal_title
Journal of molecular endocrinologyauthors
Cheung LYM,Rizzoti Kdoi
10.1530/JME-19-0276subject
Has Abstractpub_date
2020-08-01 00:00:00pages
R35-R51issue
2eissn
0952-5041issn
1479-6813pii
JME-19-0276journal_volume
65pub_type
杂志文章,评审abstract：:Fibroblast growth factor 23 (FGF23) is a phosphotropic hormone that belongs to a subfamily of endocrine FGFs with evolutionarily conserved functions in C. elegans and fruit flies. FAM20C phosphorylates FGF23 post-translationally, targeting to proteolysis through subtilisin-like proprotein convertase FURIN, resulting i...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章,评审
doi：10.1530/JME-20-0178
更新日期：2020-12-01 00:00:00
abstract：:In non-mammalian vertebrates, estrogens are key players in ovarian differentiation, but the mechanisms by which they act remain poorly understood. The present study on rainbow trout was designed to investigate whether estrogens trigger the female pathway by activating a group of early female genes (i.e. cyp19a1, foxl2...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/JME-08-0039
更新日期：2008-11-01 00:00:00
abstract：:Numerous mouse models of prostate carcinogenesis have been developed, but hitherto there has been no model in which the prostate gland could be imaged in live animals. The transgenic model generated here targeted mouse prostate gland using a firefly luciferase enzyme under the control of a small but highly active and ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.1.01722
更新日期：2005-10-01 00:00:00
abstract：:Breast cancer is a heterogeneous disease, and the complexity of breast carcinogenesis is associated with epigenetic modification. There are several major classes of epigenetic enzymes that regulate chromatin activity. This review will focus on the nine mammalian protein arginine methyltransferases (PRMTs) and the dysr...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章,评审
doi：10.1530/JME-18-0224
更新日期：2019-04-01 00:00:00
abstract：:The development and growth of prostate cancer is dependent on androgens; thus, the identification of androgen-regulated genes in prostate cancer cells is vital for defining the mechanisms of prostate cancer development and progression and developing new markers and targets for prostate cancer treatment. Glycine N-meth...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-13-0169
更新日期：2013-11-07 00:00:00
abstract：:Genetic studies have reported association of a polyglycine-encoding GGN repeat in exon 1 of the androgen receptor (AR) gene with common human traits. The polyglycine tract is located in the transactivating domain of the AR protein, suggesting an effect of repeat length on receptor function. Here, we compare the functi...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/JME-06-0072
更新日期：2007-07-01 00:00:00
abstract：:We have cloned a full length cDNA coding for the activin type IIB receptor (GactRIIB) from the goldfish ovary. GactRIIB shares 73 and 70% amino acid identity in the extracellular domain, and 78 and 80% identity in the intracellular domain with the type IIB receptors of the mouse and Xenopus respectively. The intracell...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0190047
更新日期：1997-08-01 00:00:00
abstract：:The pancreatic regenerating (reg) gene is proposed to be involved in pancreatic beta-cell growth. Up- or down-regulation of reg gene expression has been shown to parallel variations in beta-cell mass and function in the adult pancreas. In several species at least two nonallelic reg genes have been identified. In this ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0170079
更新日期：1996-08-01 00:00:00
abstract：:Decreased suppressors of cytokine signaling (SOCS) activity in human gestational tissues may play a part in the onset/progression of term labor. Since SOCS proteins negatively regulate cytokine-mediated inflammatory processes, we hypothesized that SOCS proteins are elevated in gestational tissues from spontaneous pret...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.1.01767
更新日期：2005-08-01 00:00:00
abstract：:Somatostatins (SSs) play important roles in the growth, development and metabolism of vertebrates. In this study, cDNAs for two unique somatostatin receptor variants were cloned and sequenced from rainbow trout. The two cDNAs, one consisting of 1755 bp and the other of 1743 bp, share 63.6% identity in nucleotide seque...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0320165
更新日期：2004-02-01 00:00:00
abstract：:Localization of inhibin/activin subunit mRNAs within the macaque ovary from the immediate pre-ovulatory period of the menstrual cycle, when serum immunoreactive inhibin begins to rise, to day 9 of the luteal phase, when serum inhibin concentrations are maximal, was investigated using in-situ hybridization. Ovaries wer...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0100245
更新日期：1993-06-01 00:00:00
abstract：:The parathyroid hormone (PTH)/PTH-related peptide (PTHrP) receptor regulates extracellular calcium concentrations and is therefore important for mineral homeostasis. ROS 17/2.8 cells, a rat osteoblast-like osteosarcoma cell line, express the PTH/PTHrP receptor and provide a good model for examining the transcriptional...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0250309
更新日期：2000-12-01 00:00:00
abstract：:Abnormal placental function is well-established as a major cause for poor pregnancy outcome. Placental blood flow within the maternal uteroplacental compartment, the fetoplacental circulation or both is a vital factor in mediating placental function. Impairment in flow in either or both vasculatures is a significant r...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章,评审
doi：10.1530/JME-17-0139
更新日期：2018-01-01 00:00:00
abstract：:Progesterone's ability to maintain pregnancy in eutherian mammals highlighted this steroid as the 'hormone of pregnancy'. It was the unique 'pro-gestational' bioactivity of progesterone that enabled eventual purification of this ovarian steroid to crystalline form by Willard Myron Allen in the early 1930s. While a fun...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-19-0212
更新日期：2020-07-01 00:00:00
abstract：:The main regulators of aldosterone secretion in adrenal gland zona glomerulosa (ZG) cells are the hormones angiotensin II (Ang II) and adrenocorticotrophin (ACTH) and small increases in the extracellular potassium (K(+)) concentration. The action of these agonists is mediated by different signalling systems - ACTH is ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章,评审
doi：10.1677/jme.0.0320893
更新日期：2004-06-01 00:00:00
abstract：:Hormones are dynamically collected by fenestrated capillaries to generate pulses, which are then decoded by target tissues to mount a biological response. To generate hormone pulses, endocrine systems have evolved mechanisms to tightly regulate blood perfusion and oxygenation, coordinate endocrine cell responses to se...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章,评审
doi：10.1530/JME-11-0035
更新日期：2011-08-25 00:00:00
abstract：:Basal and thyroid hormone-regulated expression of constructs containing varying lengths of 5' flanking sequence of the human glycoprotein hormone alpha subunit gene ligated to the reporter gene encoding chloramphenicol acetyl transferase (CAT) were examined in transfected pituitary GH3 cells. CAT gene expression was q...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0040187
更新日期：1990-04-01 00:00:00
abstract：:The classical concept of the renin-angiotensin system (RAS) is that of a blood-borne cascade, whose final and bioactive product, angiotensin II, plays an important endocrine role in the maintenance of blood pressure and electrolyte as well as fluid balance. In addition to this circulating RAS, there are an increasing ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章,评审
doi：10.1677/jme.0.0260155
更新日期：2001-06-01 00:00:00
abstract：:Parathyroid hormone (PTH), a powerful bone-resorbing agent, is capable of stimulating interstitial collagenase (MMP-13) mRNA production in osteoblastic cells. In this study, a PEA3 consensus binding sequence (-80; AGGAAGT) in addition to a 'TRE-like' sequence (-89; CGACTCA) in the 5' upstream regulatory region of the ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0250073
更新日期：2000-08-01 00:00:00
abstract：:Successful human embryo implantation requires a proper differentiation of endometrial stromal cells (ESCs) into decidual cells, during a process called decidualization. ESCs express specific molecules, such as prolactin, insulin-like growth factor-binding protein-1 (IGFBP-1) and connexin-43. Decidual cells are also in...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-17-0046
更新日期：2017-10-01 00:00:00
abstract：:The nona-peptide oxytocin (OT) induces contraction of the myometrium by interaction with specific plasma membrane associated OT receptors (OTR), whereas stimulation of beta2-adrenoceptors (beta2AR) causes relaxation. Homologous desensitization of the myometrium to both hormones has been described. However, a possible ...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0200261
更新日期：1998-04-01 00:00:00
abstract：:Secretion of parathyroid hormone-related protein (PTHrP) by sheep fetal parathyroid glands is reported to be an important factor in the maintenance of a placental calcium pump. The aim of the present study was to determine whether the developing rat parathyroid glands express PTHrP or parathyroid hormone (PTH), or bot...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0170149
更新日期：1996-10-01 00:00:00
abstract：:Synthesis of the uterine receptor for the hypothalamic hormone oxytocin has been induced in oocytes from Xenopus laevis previously primed with bovine endometrium mRNA. The injected oocytes responded to oxytocin by showing dose-dependent oscillations in membrane currents as recorded by the voltage-clamp method. The res...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0010077
更新日期：1988-07-01 00:00:00
abstract：:Unbiased genomic screening analyses have highlighted novel immunomodulatory properties of the active form of vitamin D, 1,25-dihydroxyvitamin D (1,25(OH)2D). However, clearer interpretation of the resulting gene expression data is limited by cell model specificity. The aim of the current study was to provide a broader...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1530/JME-17-0186
更新日期：2018-02-01 00:00:00
abstract：:Corticotrophin-releasing hormone (CRH) has been identified in several peripheral tissues, including the female reproductive organs. CRH is expressed in the placenta, myometrium, epithelial endometrium and the endometrial stromal cells at all phases of the menstrual cycle. Similarly, CRH receptors are present in pregna...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0320021
更新日期：2004-02-01 00:00:00
abstract：:In vertebrates, sex is determined essentially by two means, genetic factors located on sex chromosomes and epigenetic factors such as temperature experienced by the individual during development. Steroids, especially estrogens, are clearly involved in gonadal differentiation in non-mammalian vertebrates. In this regar...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.1.01588
更新日期：2004-12-01 00:00:00
abstract：:Soluble guanylyl cyclase is a cytosolic enzyme which catalyzes conversion of GTP to the second messenger cyclic GMP. The transcriptional regulation at the promoter levels of four soluble guanylyl cyclase subunits, termed alpha1, alpha2, beta1, and beta2, is largely unknown. In this study, we identified the transcripti...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.1.02180
更新日期：2006-12-01 00:00:00
abstract：:The peroxisome proliferator-activated receptor (PPAR) is a member of the steroid hormone receptor superfamily and is activated by a variety of fibrate hypolipidaemic drugs and non-genotoxic rodent hepatocarcinogens that are collectively termed peroxisome proliferators. A key marker of peroxisome proliferator action is...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/jme.0.0110037
更新日期：1993-08-01 00:00:00
abstract：:CRH and its structurally related peptide urocortin (Ucn) are released under stress. Ucn is a potent agonist for CRH-receptor 2 (CRH-R2), which is strongly expressed in rodent heart. Stress induces Ucn mRNA expression in the heart, where it may be cardioprotective. However, increasing evidence indicates that Ucn may al...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章
doi：10.1677/JME-08-0120
更新日期：2009-05-01 00:00:00
abstract：:About 70% of breast tumors express estrogen receptor alpha (ERα), which mediates the proliferative effects of estrogens on breast epithelial cells, and are candidates for treatment with antiestrogens, steroidal or non-steroidal molecules designed to compete with estrogens and antagonize ERs. The variable patterns of a...
journal_title：Journal of molecular endocrinology
pub_type： 杂志文章,评审
doi：10.1530/JME-16-0024
更新日期：2017-01-01 00:00:00
