Abstract:
:The development and growth of prostate cancer is dependent on androgens; thus, the identification of androgen-regulated genes in prostate cancer cells is vital for defining the mechanisms of prostate cancer development and progression and developing new markers and targets for prostate cancer treatment. Glycine N-methyltransferase (GNMT) is a S-adenosylmethionine-dependent methyltransferase that has been recently identified as a novel androgen-regulated gene in prostate cancer cells. Although the importance of this protein in prostate cancer progression has been extensively addressed, little is known about the mechanism of its androgen regulation. Here, we show that GNMT expression is stimulated by androgen in androgen receptor (AR) expressing cells and that the stimulation occurs at the mRNA and protein levels. We have identified an androgen response element within the first exon of the GNMT gene and demonstrated that AR binds to this element in vitro and in vivo. Together, these studies identify GNMT as a direct transcriptional target of the AR. As this is an evolutionarily conserved regulatory element, this highlights androgen regulation as an important feature of GNMT regulation.
journal_name
J Mol Endocrinoljournal_title
Journal of molecular endocrinologyauthors
Ottaviani S,Brooke GN,O'Hanlon-Brown C,Waxman J,Ali S,Buluwela Ldoi
10.1530/JME-13-0169subject
Has Abstractpub_date
2013-11-07 00:00:00pages
301-12issue
3eissn
0952-5041issn
1479-6813pii
JME-13-0169journal_volume
51pub_type
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