pex5 Mutants that differentially disrupt PTS1 and PTS2 peroxisomal matrix protein import in Arabidopsis.

Abstract:

:PEX5 and PEX7 are receptors required for the import of peroxisome-bound proteins containing one of two peroxisomal targeting signals (PTS1 or PTS2). To better understand the role of PEX5 in plant peroxisomal import, we characterized the Arabidopsis (Arabidopsis thaliana) pex5-10 mutant, which has a T-DNA insertion in exon 5 of the PEX5 gene. Sequencing results revealed that exon 5, along with the T-DNA, is removed in this mutant, resulting in a truncated pex5 protein. The pex5-10 mutant has germination defects and is completely dependent on exogenous Suc for early seedling establishment, based on poor utilization of seed-storage fatty acids. This mutant also has delayed development and reduced fertility, although adult pex5-10 plants appear normal. Peroxisomal metabolism of indole-3-butyric acid, propionate, and isobutyrate also is disrupted. The pex5-10 mutant has reduced import of both PTS1 and PTS2 proteins, and enzymatic processes that occur in peroxisomes are disrupted. To specifically study the import and importance of PTS1 proteins, we made a truncated PEX5 construct lacking the PTS1-binding region (PEX5(454)). Transformation of this construct into pex5-10 resulted in the rescue of PTS2 import, thereby creating a line with PTS1-specific import defects. The pex5-10 (PEX5(454)) plants still had developmental defects, although restoring PTS2 import resulted in a less severe mutant phenotype. Comparison of pex5-10 and pex5-10 (PEX5(454)) phenotypes can separate the import mechanisms for enzymes acting in different peroxisomal processes, including indole-3-butyric acid/2,4-dichlorophenoxybutyric acid oxidation, isobutyrate and propionate metabolism, and photorespiration.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Khan BR,Zolman BK

doi

10.1104/pp.110.162479

subject

Has Abstract

pub_date

2010-12-01 00:00:00

pages

1602-15

issue

4

eissn

0032-0889

issn

1532-2548

pii

pp.110.162479

journal_volume

154

pub_type

杂志文章
  • Photosynthetic pigment localization and thylakoid membrane morphology are altered in Synechocystis 6803 phycobilisome mutants.

    abstract::Cyanobacteria are oxygenic photosynthetic prokaryotes that are the progenitors of the chloroplasts of algae and plants. These organisms harvest light using large membrane-extrinsic phycobilisome antenna in addition to membrane-bound chlorophyll-containing proteins. Similar to eukaryotic photosynthetic organisms, cyano...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.192849

    authors: Collins AM,Liberton M,Jones HD,Garcia OF,Pakrasi HB,Timlin JA

    更新日期:2012-04-01 00:00:00

  • Chloroplast Membrane Remodeling during Freezing Stress Is Accompanied by Cytoplasmic Acidification Activating SENSITIVE TO FREEZING2.

    abstract::Low temperature is a seasonal abiotic stress that restricts native plant ranges and crop distributions. Two types of low-temperature stress can be distinguished: chilling and freezing. Much work has been done on the mechanisms by which chilling is sensed, but relatively little is known about how plants sense freezing....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00286

    authors: Barnes AC,Benning C,Roston RL

    更新日期:2016-07-01 00:00:00

  • Effects of inorganic phosphate on the light dependent thylakoid energization of intact spinach chloroplasts.

    abstract::The light dependent energization of the thylakoid membrane was analyzed in isolated intact spinach (Spinacia oleracea L.) chloroplasts incubated with different concentrations of inorganic phosphate (Pi). Two independent methods were used: (a) the accumulation of [(14)C]5,5-dimethyl-2,4-oxazolidinedione and [(14)C] met...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.1.221

    authors: Heineke D,Stitt M,Heldt HW

    更新日期:1989-09-01 00:00:00

  • CO(2) Metabolism in Corn Roots. III. Inhibition of P-enolpyruvate Carboxylase by l-malate.

    abstract::Phosphoenolpyruvate carboxylase was purified from corn root tips about 80-fold by centrifugation, ammonium sulfate fractionation, and anion exchange and gel filtration chromatography. The resulting preparation was essentially free from malate dehydrogenase, isocitrate dehydrogenase, malate enzyme, NADH oxidase, and py...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.12.1919

    authors: Ting IP

    更新日期:1968-12-01 00:00:00

  • Chloroplast NADPH-thioredoxin reductase interacts with photoperiodic development in Arabidopsis.

    abstract::Chloroplast NADPH-thioredoxin reductase (NTRC) belongs to the thioredoxin systems that control crucial metabolic and regulatory pathways in plants. Here, by characterization of T-DNA insertion lines of NTRC gene, we uncover a novel connection between chloroplast thiol redox regulation and the control of photoperiodic ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.133777

    authors: Lepistö A,Kangasjärvi S,Luomala EM,Brader G,Sipari N,Keränen M,Keinänen M,Rintamäki E

    更新日期:2009-03-01 00:00:00

  • The procambium specification gene Oshox1 promotes polar auxin transport capacity and reduces its sensitivity toward inhibition.

    abstract::The auxin-inducible homeobox gene Oshox1 of rice (Oryza sativa) is a positive regulator of procambial cell fate commitment, and its overexpression reduces the sensitivity of polar auxin transport (PAT) to the PAT inhibitor 1-N-naphthylphthalamic acid (NPA). Here, we show that wild-type rice leaves formed under conditi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.009167

    authors: Scarpella E,Boot KJ,Rueb S,Meijer AH

    更新日期:2002-11-01 00:00:00

  • Properties of Ornithine Carbamoyltransferase from Pisum sativum L.

    abstract::Some properties of ornithine carbamoyltransferase from chloroplasts isolated from leaves of Pisum sativum L. (cv Marzia) were compared with those of the enzyme partially purified (316-fold) from shoots of seedlings after 3 weeks of cultivation.Both preparations showed a pH optimum at pH 8.3 and had the same affinity t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.3.695

    authors: de Ruiter H,Kollöffel C

    更新日期:1985-03-01 00:00:00

  • Chilling-Induced Lipid Degradation in Cucumber (Cucumis sativa L. cv Hybrid C) Fruit.

    abstract::Chilling at 4 degrees C in the dark induced lipid degradation in cucumber (Cucumis sativa L.) fruit upon rewarming at 14 degrees C. Rates of ethane evolution by fruits rewarmed after 3 days of chilling were up to four-fold higher than those evolved by unchilled (14 degrees C) fruits (0.02-0.05 picomoles gram fresh wei...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.3.1049

    authors: Parkin KL,Kuo SJ

    更新日期:1989-07-01 00:00:00

  • Ribulose-1,5-bisphosphate Carboxylase/Oxygenase and Polyphenol Oxidase in the Tobacco Mutant Su/su and Three Green Revertant Plants.

    abstract::Ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) was crystallized from a heterozygous tobacco (Nicotiana tabacum L.) aurea mutant (Su/su), its wild-type sibling (su/su), and green revertant plants regenerated from green spots found on leaves of haploid Su plants. No differences were found in the specific ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.5.828

    authors: Koivuniemi PJ,Tolbert NE,Carlson PS

    更新日期:1980-05-01 00:00:00

  • Xanthophylls and abscisic Acid biosynthesis in water-stressed bean leaves.

    abstract::Experiments were designed to obtain evidence about the possible role of xanthophylls as abscisic acid (ABA) precursors in water-stressed leaves of Phaseolus vularis L. Leaves were exposed to (14)CO(2) and the specific activities of several major leaf xanthophylls and stress-induced ABA were determined after a chase in...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.4.910

    authors: Li Y,Walton DC

    更新日期:1987-12-01 00:00:00

  • Differential Response in the Water Status of Immature and Mature Fronds of the Ostrich Fern (Matteuccia struthiopteris [L.] Todaro) to a Mild Water Stress.

    abstract::Experiments were conducted in growth chambers to examine the effect of a mild water stress (-200 kilopascals polyethylene glycol) on frond elongation and water status of the ostrich fern (Matteuccia struthiopteris [L.] Todaro). Measurements were taken for two days, starting one day after the application of polyethylen...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.1.96

    authors: Prange RK,Ormrod DP

    更新日期:1983-05-01 00:00:00

  • Analysis of ethylene signal-transduction kinetics associated with seedling-growth response and chitinase induction in wild-type and mutant arabidopsis.

    abstract::Kinetic aspects of ethylene-mediated signal transduction leading to seedling-growth inhibition and chitinase induction in Arabidopsis were investigated by the introduction of defined mutations in components of these pathways. Dose-response analysis of wild-type responses indicated that the rate-limiting steps for seed...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.2.597

    authors: Chen QG,Bleecker AB

    更新日期:1995-06-01 00:00:00

  • Ligand Specificity of Bean Leaf Soluble Auxin-binding Protein.

    abstract::The soluble bean leaf auxin-binding protein (ABP) has a high affinity for a range of auxins including indole-3-acetic acid (IAA), alpha-napthaleneacetic acid, phenylacetic acid, 2,4,5-trichlorophenoxyacetic acid, and structurally related auxins. A large number of nonauxin compounds that are nevertheless structurally r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.1.112

    authors: Wardrop AJ,Polya GM

    更新日期:1980-07-01 00:00:00

  • Effect of asymmetric auxin application on Helianthus hypocotyl curvature.

    abstract::Indole-3-acetic acid was applied asymmetrically to the hypocotyls of sunflower (Helianthus annuus L.) seedlings. After 5 hours on a clinostat, auxin gradients as small as 1 to 1.3 produced substantial (more than 60 degrees) hypocotyl curvature. This result suggests the asymmetric growth underlying hypocotyl gravitro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.2.466

    authors: Migliaccio F,Rayle DL

    更新日期:1989-01-01 00:00:00

  • Messenger RNA-binding properties of nonpolysomal ribonucleoproteins from heat-stressed tomato cells

    abstract::Most cells experiencing heat stress reprogram their translational machinery to favor the synthesis of heat-stress proteins. Translation of other transcripts is almost completely repressed, but most untranslated messengers are not degraded. In contrast to yeast, Drosophila melanogaster, and HeLa cells, plant cells stor...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.120.1.23

    authors: Stuger R,Ranostaj S,Materna T,Forreiter C

    更新日期:1999-05-01 00:00:00

  • DNA free energy-based promoter prediction and comparative analysis of Arabidopsis and rice genomes.

    abstract::The cis-regulatory regions on DNA serve as binding sites for proteins such as transcription factors and RNA polymerase. The combinatorial interaction of these proteins plays a crucial role in transcription initiation, which is an important point of control in the regulation of gene expression. We present here an analy...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.167809

    authors: Morey C,Mookherjee S,Rajasekaran G,Bansal M

    更新日期:2011-07-01 00:00:00

  • The wheat mitogen-activated protein kinases TaMPK3 and TaMPK6 are differentially regulated at multiple levels during compatible disease interactions with Mycosphaerella graminicola.

    abstract::Many race- or isolate-specific disease resistance responses of plants toward pathogens (incompatible interactions) invoke hypersensitive response (HR)-like programmed cell death (PCD) and the coordinated activation of mitogen-activated protein kinases homologous with Arabidopsis (Arabidopsis thaliana) AtMPK6 and AtMPK...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.119511

    authors: Rudd JJ,Keon J,Hammond-Kosack KE

    更新日期:2008-06-01 00:00:00

  • The synthetic elicitor 3,5-dichloroanthranilic acid induces NPR1-dependent and NPR1-independent mechanisms of disease resistance in Arabidopsis.

    abstract::Immune responses of Arabidopsis (Arabidopsis thaliana) are at least partially mediated by coordinated transcriptional up-regulation of plant defense genes, such as the Late/sustained Up-regulation in Response to Hyaloperonospora parasitica (LURP) cluster. We found a defined region in the promoter of the LURP member Ca...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.133678

    authors: Knoth C,Salus MS,Girke T,Eulgem T

    更新日期:2009-05-01 00:00:00

  • A new 1-aminocyclopropane-1-carboxylic acid-conjugating activity in tomato fruit.

    abstract::A new conjugate, 1-(gamma-L-glutamylamino)cyclopropane-1-carboxylic acid (GACC), of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) is identified. The only previously identified conjugate of ACC is 1-(malonylamino)cyclopropane-1-carboxylic acid (MACC). GACC, not MACC, was the major conjugate formed ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.3.917

    authors: Martin MN,Cohen JD,Saftner RA

    更新日期:1995-11-01 00:00:00

  • Reversible o(2) inhibition of nitrogenase activity in attached soybean nodules.

    abstract::Various forms of stress result in decreased O(2) permeability or decreased capacity to consume O(2) in legume root nodules. These changes alter the nodule interior O(2) concentration (O(i)). To determine the relationship between O(i) and nitrogenase activity in attached soybean (Glycine max) nodules, we controlled O(i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.4.1863

    authors: Denison RF,Witty JF,Minchin FR

    更新日期:1992-12-01 00:00:00

  • A methyl viologen-resistant mutant of Arabidopsis, which is allelic to ozone-sensitive rcd1, is tolerant to supplemental ultraviolet-B irradiation.

    abstract::To better understand the role of active oxygen species (AOS) in acquired resistance to increased levels of ultraviolet (UV)-B irradiation in plants, we isolated an Arabidopsis mutant that is resistant to methyl viologen, and its sensitivity to UV-B was investigated. A complementation test revealed that the obtained mu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.033480

    authors: Fujibe T,Saji H,Arakawa K,Yabe N,Takeuchi Y,Yamamoto KT

    更新日期:2004-01-01 00:00:00

  • Histochemical and biochemical observations on storage protein metabolism and protein body autolysis in cotyledons of germinating mung beans.

    abstract::Storage protein hydrolysis in the cotyledons of germinating mung beans (Phaseolus aureus Roxb.) was examined by histochemical techniques, and the autolytic capacity of isolated protein bodies was studied with biochemical methods. The localization of endopeptidase activity within the cotyledons was studied using an Ind...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.56.2.292

    authors: Harris N,Chrispeels MJ

    更新日期:1975-08-01 00:00:00

  • Variations in the composition of gelling agents affect morphophysiological and molecular responses to deficiencies of phosphate and other nutrients.

    abstract::Low inorganic phosphate (Pi) availability triggers an array of spatiotemporal adaptive responses in Arabidopsis (Arabidopsis thaliana). There are several reports on the effects of Pi deprivation on the root system that have been attributed to different growth conditions and/or inherent genetic variability. Here we sho...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.136184

    authors: Jain A,Poling MD,Smith AP,Nagarajan VK,Lahner B,Meagher RB,Raghothama KG

    更新日期:2009-06-01 00:00:00

  • Oxygen Uptake and Photosynthesis of the Red Macroalga, Chondrus crispus, in Seawater: Effects of Oxygen Concentration.

    abstract::With an experimental system developed for aquatic plants using the mass spectrometry technique and infrared gas analysis of CO(2), we studied the responses to various O(2) concentrations of gas exchanges with the red macroalga Chondrus crispus S. The results were as follows. (a) Irrespective of the CO(2) concentration...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.3.545

    authors: Brechignac F,Andre M

    更新日期:1985-07-01 00:00:00

  • The chemically inducible plant cytochrome P450 CYP76B1 actively metabolizes phenylureas and other xenobiotics

    abstract::Cytochrome P450s (P450s) constitute one of the major classes of enzymes that are responsible for detoxification of exogenous molecules both in animals and plants. On the basis of its inducibility by exogenous chemicals, we recently isolated a new plant P450, CYP76B1, from Jerusalem artichoke (Helianthus tuberosus) and...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.118.3.1049

    authors: Robineau T,Batard Y,Nedelkina S,Cabello-Hurtado F,LeRet M,Sorokine O,Didierjean L,Werck-Reichhart D

    更新日期:1998-11-01 00:00:00

  • Calcium in the regulation of gravitropism by light.

    abstract::The red light requirement for positive gravitropism in roots of corn (Zea mays cv "Merit") provides an entry for examining the participation of calcium in gravitropism. Applications of calcium chelators inhibit the light response. Calcium channel blockers (verapamil, lanthanum) can also inhibit the light response, a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.4.1276

    authors: Perdue DO,LaFavre AK,Leopold AC

    更新日期:1988-01-01 00:00:00

  • Arabidopsis MPK3 and MPK6 play different roles in basal and oligogalacturonide- or flagellin-induced resistance against Botrytis cinerea.

    abstract::Mitogen-activated protein kinases (MAPKs) are fundamental components of the plant innate immune system. MPK3 and MPK6 are Arabidopsis (Arabidopsis thaliana) MAPKs activated by pathogens and elicitors such as oligogalacturonides (OGs), which function as damage-associated molecular patterns, and flg22, a well-known micr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.174003

    authors: Galletti R,Ferrari S,De Lorenzo G

    更新日期:2011-10-01 00:00:00

  • Ethylene insensitivity conferred by the Green-ripe and Never-ripe 2 ripening mutants of tomato.

    abstract::The ripening of a fleshy fruit represents the summation of an array of biochemical processes that are regulated by interactions between developmental programs and environmental inputs. Analysis of tomato (Solanum lycopersicum) mutants and inhibitor studies indicate that ethylene is necessary for full development of th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.057745

    authors: Barry CS,McQuinn RP,Thompson AJ,Seymour GB,Grierson D,Giovannoni JJ

    更新日期:2005-05-01 00:00:00

  • Free amino Acid composition of leaf exudates and Phloem sap : a comparative study in oats and barley.

    abstract::Comparisons were made between the free amino acid composition in leaf exudates and that in pure phloem sap, using twin samples taken from a single leaf of two oat (Avena sativa L.) and three barley (Hordeum vulgare L.) varieties. Leaf exudate was collected in a 5 mm EDTA-solution (pH 7.0) from cut leaf blades and phlo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.92.1.222

    authors: Weibull J,Ronquist F,Brishammar S

    更新日期:1990-01-01 00:00:00

  • Pyrophosphate Fructose-6-P 1-Phosphotransferase from Tomato Fruit : Evidence for Change during Ripening.

    abstract::Three forms of pyrophosphate fructose-6-phosphate 1-phosphotransferase (PFP) were purified from both green and red tomato (Lycopersicon esculentum) fruit: (a) a classical form (designated Q(2)) containing alpha- (66 kilodalton) and beta- (60 kilodalton) subunits; (b) a form (Q(1)) containing a beta-doublet subunit; an...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.2.499

    authors: Wong JH,Kiss F,Wu MX,Buchanan BB

    更新日期:1990-10-01 00:00:00