Abstract：
:QuEChERS sample preparation was optimized using solvent extraction with acetonitrile and dispersive-solid phase extraction with primary and secondary amine sorbents, and validated for high-performance liquid chromatographic determination of nine steroids commonly used to adulterate herbal medicines: such as triamcinolone, prednisolone, hydrocortisone, methylprednisolone, betamethasone, dexamethasone, beclomethasone, fludrocortsone acetate and cortisone acetate. Satisfactory extraction recoveries of 91-113% for all nine steroids were obtained, along with an acceptable precision in extraction recoveries shown by R.S.D. of ≤4.6 and 3.2% for intraday and interday, respectively. The QuEChERS sample preparation developed here allows the reliable detection of adulterated steroids with the limits of detection in the range of 0.06-0.17ppm. Adulterated steroids in three out of six real commercial liquid herbal medicines were found, such as 1.6 and 8.8ppm dexamethasone and 0.43ppm prednisolone.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Klinsunthorn N,Petsom A,Nhujak Tdoi
10.1016/j.jpba.2011.03.046subject
Has Abstractpub_date
2011-07-15 00:00:00pages
1175-8issue
5eissn
0731-7085issn
1873-264Xpii
S0731-7085(11)00200-7journal_volume
55pub_type
杂志文章abstract：:In recent years, dietary supplements and herbal medicines are increasing in popularity all over the world. However, it is problematic that some manufacturers illegally included synthetic drugs in their products. Due to the extremely complex matrices of those products, most existing methods for screening illegal adulte...
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