Abstract:
:A capillary electrophoresis method using cyclodextrins as the chiral selectors was developed for the determination of carvedilol enantiomers in serum. Several types of cyclodextrins were evaluated. The effect of cyclodextrin concentration on enantiomer resolution was investigated. Best results were obtained using 10 mM hydroxypropyl-beta-cyclodextrin in the run buffer. The effect of voltage on efficiency was assessed. Other electrophoretic conditions were optimized. The method was validated for carvedilol enantiomers in serum. Linearity of detection was assessed over the concentration range of 50-4000 ng/ml of each enantiomer in serum. Intra- and inter-assay variability obtained were under 8% for both enantiomers.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Clohs L,McErlane KMdoi
10.1016/s0731-7085(00)00468-4subject
Has Abstractpub_date
2001-02-01 00:00:00pages
545-54issue
4eissn
0731-7085issn
1873-264Xpii
S0731-7085(00)00468-4journal_volume
24pub_type
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