Analysis of diltiazem in Lipoderm transdermal gel using reversed-phase high-performance liquid chromatography applied to homogenization and stability studies.

Abstract:

:A simple and novel method for the extraction and quantification of diltiazem hydrochloride was developed and applied to homogenization and stability studies. The method used solid phase extraction coupled with reversed-phase high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. Validation showed inter-day recoveries ranging from 84.00 to 96.52% with relative standard deviations ranging from 12.01 to 15.94%. Intra-day recoveries ranged from 67.95 to 106.1% with relative standard deviations less than 5%. The method showed excellent linearity from 50 to 250 mg/ml in undiluted gel (R2 = 0.996). The homogenization study showed good homogenization using both 50 and 100 depression techniques. Diltiazem was stable at a concentration of 246 mg/ml for 30 days and at a concentration of 99.6 mg/ml for 60 days no matter the storage conditions explored in this study.

journal_name

J Pharm Biomed Anal

authors

Buur JL,Baynes RE,Yeatts JL,Davidson G,Defrancesco TC

doi

10.1016/j.jpba.2004.11.053

subject

Has Abstract

pub_date

2005-06-01 00:00:00

pages

60-5

issue

1

eissn

0731-7085

issn

1873-264X

pii

S0731-7085(04)00642-9

journal_volume

38

pub_type

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