A yeast estrogen screen without extraction provides fast, reliable measures of estrogenic activity.

Abstract:

:Yeast estrogen screen (YES) has been used since 1996 as a bioassay to quantify activity in wastewater. Here we present a modification of YES to measure estrogenic activity in water. This modification, called yeast estrogen screen no extraction (YESne), is faster and easier than the common method. The modified method can detect 17β-estradiol equivalent concentrations down to 1.1 ng/L. The median effective concentration value (EC50) is 1.2E-10. It detected average influent concentrations of 16.4 and 17.5 ng/L of 17β-estradiol equivalent at four Lehigh Valley, Pennsylvania, USA, wastewater treatment plants on September 18 and October 23, 2008, respectively, and average effluent concentrations of 5.1 and 8.1 ng/L of 17β-estradiol equivalent at the same plants on the two dates, respectively. Reduction in 17β-estradiol equivalent activity for the four wastewater treatment plants averaged 67.8 and 52.3%, respectively, for the September 18 and October 23 samples. The YESne is a simple, quick method for quantifying estrogenic activity that has been used successfully in nonmajor undergraduate classes and could be adapted by wastewater treatment plant laboratory technicians to measure influent and effluent estrogenicity on a regular basis. This practice will greatly increase our knowledge base of estrogenicity in wastewater before and after treatment.

journal_name

Environ Toxicol Chem

authors

Colosi JC,Kney AD

doi

10.1002/etc.618

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

2261-9

issue

10

eissn

0730-7268

issn

1552-8618

journal_volume

30

pub_type

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