Abstract:
INTRODUCTION:Opportunistic fungal infections in immunocompromised hosts are caused by Candida species, and the majority of such infections are due to Candida albicans. However, the emerging pathogen Candida dubliniensis demonstrates several phenotypic characteristics in common with C. albicans, such as production of germ tubes and chlamydospores, calling attention to the development of stable resistance to fluconazole in vitro. The aim of this study was to evaluate the performance of biochemistry identification in the differentiating between C. albicans and C. dubliniensis, by phenotyping of yeast identified as C. albicans. METHODS:Seventy-nine isolates identified as C. albicans by the API system ID 32C were grown on Sabouraud dextrose agar at 30°C for 24-48h and then inoculated on hypertonic Sabouraud broth and tobacco agar. RESULTS:Our results showed that 17 (21.5%) isolates were growth-inhibited on hypertonic Sabouraud broth, a phenotypic trait inconsistent with C. albicans in this medium. However, the results observed on tobacco agar showed that only 9 (11.4%) of the growth-inhibited isolates produced characteristic colonies of C. dubliniensis (rough colonies, yellowish-brown with abundant fragments of hyphae and chlamydospores). CONCLUSIONS:The results suggest that this method is a simple tool for screening C. albicans and non-albicans yeast and for verification of automated identification.
journal_name
Rev Soc Bras Med Tropjournal_title
Revista da Sociedade Brasileira de Medicina Tropicalauthors
Silveira-Gomes F,Sarmento DN,Espírito-Santo EP,Souza Nde O,Pinto TM,Marques-da-Silva SHdoi
10.1590/s0037-86822011000400011subject
Has Abstractpub_date
2011-07-01 00:00:00pages
457-60issue
4eissn
0037-8682issn
1678-9849pii
S0037-86822011000400011journal_volume
44pub_type
杂志文章abstract::The frequency with which condoms are used in sex relations by subjects with HIV was determined by interviewing 132 individuals, 82 men and 50 women, most of them from São Paulo state and some from other regions of the country, all of them seen at an outpatient clinic of the School of Medicine in Botucatu. The women we...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
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doi:10.1590/s0037-86822006000100013
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86821999000500003
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章,评审
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更新日期:2000-05-01 00:00:00
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章,随机对照试验
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更新日期:2019-04-11 00:00:00
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
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更新日期:2020-10-21 00:00:00
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822011000400021
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journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822007000400006
更新日期:2007-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-12-21 00:00:00