Abstract：
OBJECTIVE:The aim of this study was to detect the relationship between long-chain non-coding RNA (lncRNA) NEAT1 and microRNA-1224 (miR-1224) in lung cancer and to explore its underlying mechanism. MATERIALS AND METHODS:The expression levels of lncRNA NEAT1 and miR-1224 in lung cancer tissues and cells were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The interaction between lncRNA NEAT1 with miR-1224, miR-1224, and KLF3 was detected by Dual-Luciferase Reporter Gene Assay. MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and flow cytometry were used to detect the changes in the proliferative and apoptosis abilities of lung cancer cells after silencing lncRNA NEAT1 or up-regulating miR-1224, respectively. RESULTS:Compared with adjacent normal tissues, lncRNA NEAT1 was significantly up-regulated, while miR-1224 was significantly down-regulated in lung cancer tissues. LncRNA NEAT1 could specifically bind to the 3'UTR of miR-1224 and regulate its expression. The inhibition of lncRNA NEAT1 remarkably reduced the proliferation and enhanced the apoptosis of lung cancer cells. However, the upregulation of the expression of miR-1224 level could significantly inhibit proliferation and promote the apoptosis rate of lung cancer cells. Furthermore, miR-1224 could downregulate KLF3 expression by directly binding to its 3'UTR. CONCLUSIONS:LncRNA NEAT1 can sponge the expression of miR-1224, thereby affecting the proliferation and apoptosis of lung cancer.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Yu PF,Wang Y,Lv W,Kou D,Hu HL,Guo SS,Zhao YJdoi
10.26355/eurrev_201910_19151subject
Has Abstractpub_date
2019-10-01 00:00:00pages
8403-8410issue
19eissn
1128-3602issn
2284-0729journal_volume
23pub_type
杂志文章abstract：OBJECTIVE:Polycystic ovarian syndrome and obesity contribute to the metabolic complications for women of reproductive age. The aim of present study was to analyze the effect of abdominal obesity expressed using waist/hip ratio (WHR) in patients with polycystic ovary syndrome on metabolic parameters. PATIENTS AND METHO...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2017-11-01 00:00:00
abstract：OBJECTIVE:Long noncoding RNA (lncRNA) GIHCG has been reported as an oncogene in hepatocellular carcinoma. However, the expression, roles, and clinical values of GIHCG in renal cell carcinoma (RCC) remain unclear. The aim of this study was to investigate the expression, roles, diagnostic and prognostic values of GIHCG i...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201801_14099
更新日期：2018-01-01 00:00:00
abstract：:The pandemic influenza A H1N1 will affect millions of subjects. This influenza can cause respiratory complications with possible death. We have described two case reports of acute severe asthma exacerbation combined to influenza A H1N1, caracterized by severe respiratory failure. The diagnosis of influenza A H1N1 was ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2010-05-01 00:00:00
abstract：OBJECTIVE:Glioma is one common intracranial malignancy. Recently, there has been a large volume of published studies describing the functions of microRNAs as potential diagnostic markers for glioma. Data from several sources revealed that miR-378 played crucial roles in multiple tumors. However, much uncertainty still ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201806_15268
更新日期：2018-06-01 00:00:00
abstract：OBJECTIVE:The RIB43A domain with coiled-coils 2 (RIBC2) encodes an uncharacterized vertebrate protein exhibiting similarity with Chlamydomonas protofilament ribbon proteins, required for ciliary motility. To date, no functional variants capable of triggering a change in the expression of RIBC2 have been reported. PATI...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202004_20851
更新日期：2020-04-01 00:00:00
abstract：OBJECTIVE:Asthma is the most common chronic airway inflammatory disease. Sirtuin 1 (SIRT1) exerts a crucial effect on regulating chronic inflammatory responses. Therefore, this study aims to explore the effect of SIRT1 on the pathogenesis of asthma. MATERIALS AND METHODS:Serum level of SIRT1 in asthma patients and hea...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201906_18085
更新日期：2019-06-01 00:00:00
abstract：OBJECTIVE:To explore the effects of remote ischemic preconditioning on myocardial injury and prognosis after percutaneous coronary intervention (PCI) in patients with acute coronary syndrome. PATIENTS AND METHODS:The study was a single center, prospective, randomized, controlled study. A total of 184 patients with uns...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2017-10-01 00:00:00
abstract：OBJECTIVE:To investigate the impact of miR-124Aa on the proliferation, invasion and cytokine excretion of rheumatoid arthritis synovial fibroblasts (RASFs) in patients with rheumatoid arthritis (RA). PATIENTS AND METHODS:RASFs were separated for in-vitro culture, and transfected using lipidosome that connected with ch...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201807_15515
更新日期：2018-07-01 00:00:00
abstract：OBJECTIVE:To explore the possible role and mechanism of miR-497 in cutaneous squamous cell carcinoma. PATIENTS AND METHODS:Quantitative Real-time polymerase chain reaction (qRT-PCR) was used to detect miR-497 and FAM114A2 expression level in 38 cases of cutaneous squamous cell carcinoma (CSCC) and 22 normal skin tissu...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201811_16272
更新日期：2018-11-01 00:00:00
abstract：OBJECTIVE:To investigate the effect of Lactobacillus rhamnosus GR-1 on atherosclerotic progression in apolipoprotein-E knockout (ApoE-/-) mice fed with a high-fat diet and the underlying mechanisms of its action. MATERIALS AND METHODS:Eight-week-old ApoE-/- mice were treated with Lactobacillus rhamnosus GR-1 daily for...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201904_17722
更新日期：2019-04-01 00:00:00
abstract：BACKGROUND:The diagnosis of spondylodiscitis can be difficult, because the patients history, subjective symptoms and physical findings are often inconclusive, particularly in the early stages. AIM:To perform an overview on the role of nuclear medicine procedures with single photon emission tomography (SPET) and positr...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,评审
doi：
更新日期：2012-04-01 00:00:00
abstract：OBJECTIVE:Observe the expression and distribution of HMGB1 in Sombati's cell model and kainic acid-induced epileptic rats' model. MATERIALS AND METHODS:Dissociated hippocampal neurons from neonatal SD rats and cultured those for 9 days, then changed medium to Mg2+-free medium for 3 hours to induce Sombati's cell model...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2015-08-01 00:00:00
abstract：OBJECTIVE:Pain relief is a very important aspect in Pediatrician's clinical practice. It is often thought that young children, particularly infants, do not perceive as much pain as adults because of their immature nervous system and that untreated pain would not have adverse long-term consequences. Instead, it has been...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,评审
doi：10.26355/eurrev_201801_14120
更新日期：2018-01-01 00:00:00
abstract：OBJECTIVE:To explore the role of Id1 in ovarian cancer cell proliferation, invasion and apoptosis. MATERIALS AND METHODS:Lentivirus-based shRNA vectors were constructed to knockdown Id1 expression in SKOV3 ovarian cancer cells. The proliferation, invasion ability and apoptosis of SKOV3 cells were evaluated by CCK-8 as...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2016-07-01 00:00:00
abstract：OBJECTIVE:Cervical cancer rate is increasing recently. LncRNA UCA1 plays a role in gynecological tumors, but its expression and mechanism in cervical cancer have not yet been elucidated. PATIENTS AND METHODS:The tumor tissues and adjacent tissues of cervical cancer patients were collected to measure LncRNA UCA1 and mi...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202010_23197
更新日期：2020-10-01 00:00:00
abstract：OBJECTIVE:Lung cancer is the leading cause of cancer-related mortality. Over 80% of all lung cancer cases are non-small-cell lung cancer (NSCLC) and approximately 5% of NSCLC patients are positive for anaplastic lymphoma kinase (ALK) gene rearrangement or fusion with echinoderm microtubule-associated protein-like 4 (EM...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,meta分析,评审
doi：
更新日期：2017-08-01 00:00:00
abstract：OBJECTIVE:To investigate the risk factors related to mortality in severe polytrauma patients with acute hypoxemic respiratory failure (AHRF). PATIENTS AND METHODS:From December 2011 to December 2014, we identified and intubated 524 traumatic AHRF patients in a level 1 trauma centers. Amongst those, we enrolled seventy...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2015-10-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to explore the clinical significance of miR-145-5p expression in gastric cancer (GC). PATIENTS AND METHODS:Expression of miR-145-5p was evaluated by qRT- PCR in tumor and normal gastric tissues in 145 GC patients. The correlation between the miR-145-5p expression and clinicopatholog...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2016-07-01 00:00:00
abstract：OBJECTIVE:To investigate the usefulness of 18F-FDG PET/CT imaging in the diagnosis of lymph node metastasis of esophageal carcinoma and to compare its results with pathological findings. PATIENTS AND METHODS:This study examined 43 cases of patients (32 males and 11 females, aged 54 ± 13 years old) being diagnosed with...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2016-04-01 00:00:00
abstract：:Catheter-related bloodstream infections (CRBI) represent a frequent complication of immune-compromised hosts with a high mortality rate. In this setting, opportunistic pathogens can create a biofilm on implanted devices, being the source of infection. We provide a mini-review of the literature, starting from the descr...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,评审
doi：
更新日期：2015-07-01 00:00:00
abstract：AIMS AND BACKGROUND:We investigated the efficacy of docetaxel plus prednisone in Italian patients with metastatic hormone-refractory prostate cancer (mHRPC). METHODS:Twenty four patients with mHRPC received docetaxel 75 mg/m2 every 3 weeks plus prednisone 5 mg twice daily for up to six cycles. The primary endpoint was...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2011-03-01 00:00:00
abstract：OBJECTIVE:We aimed to study the correlations of angiotensin II receptor type 1 (AGTR1) gene polymorphisms with the occurrence and development of eclampsia. PATIENTS AND METHODS:A total of 200 pregnant women with eclampsia admitted to our hospital from January 1, 2017 to September 30, 2019 were collected as observation...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202012_24160
更新日期：2020-12-01 00:00:00
abstract：BACKGROUND:Coronary artery disease (CAD) is the most common cause of hospitalization and mortality in many industrialized countries. We analysed the diagnostic accuracy of multi-detector row spiral computed tomography (MDCT) in determining mid- to high-grade coronary artery stenoses (> 50%). METHODS:Sixty-nine patient...
journal_title：European review for medical and pharmacological sciences
pub_type： 临床试验,杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：BACKGROUND:Osteosarcoma is an aggressive cancerous neoplasm arising from primitive transformed cells of mesenchymal origin that exhibit osteoblastic differentiation and produce malignant osteoid. With the rapid development of tumor molecular biology, gene and viral therapy, a highly promising strategy for the treatment...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2013-03-01 00:00:00
abstract：OBJECTIVE:Postpartum hemorrhage (PPH) is one of the major preventable causes of maternal death in developing countries. Although the non-parenteral use of misoprostol is a big advantage especially in home births, its benefits in prevention of PPH is still debating. We aimed to assess the effect and side-effects of prop...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,随机对照试验
doi：
更新日期：2015-01-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to elucidate the expression pattern and potential function of LINC01116 in regulating the progression of osteosarcoma. PATIENTS AND METHODS:Expression levels of LINC01116 in osteosarcoma tissues (n=52) and adjacent normal tissues (n=52) were detected by quantitative Real-time polyme...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201908_18720
更新日期：2019-08-01 00:00:00
abstract：BACKGROUND:Atherosclerotic ischemic renal disease is a frequent cause of end-stage renal failure. Correction of renal artery stenosis (RAS) may fail to stabilize or improve renal function. AIMS OF THE STUDY:Carotid and aortic Intima media thickness (IMT), resistance renal resistance index (RI), arterial blood pressure...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2013-02-01 00:00:00
abstract：BACKGROUND:Coronary artery bypass grafting (CABG) is a revascularization technique that has been reported as having satisfactory survival rates in Hemodialysis (HD) dependent patients. AIM:The aim of this study was to determine morbidity and mortality of isolated CABG in HD-dependent patients and compare with non- ren...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2013-10-01 00:00:00
abstract：OBJECTIVE:Gallbladder carcinoma is a malignant tumor in the bile duct with poor prognosis. Although aberrant expression of miR-335 has been reported in the tumor tissues of gallbladder carcinoma, the biological role of miR-335 was still largely unknown. This study was intended to explore the role of miR-335 in the prog...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201911_19546
更新日期：2019-11-01 00:00:00
abstract：:Central sleep apnea is a breathing disorder that manifests as repetitive cessation of the breath during the sleep. The occurrence of breathing disorders after cervical laminectomy has been exceptionally described as a complication after cervical decompressive laminectomy for cervical stenotic myelopathy. In 1994, Naim...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
