Effect of bioemulsificant exopolysaccharide (EPS₂₀₀₃) on microbial community dynamics during assays of oil spill bioremediation: a microcosm study.

Abstract:

:Microcosms experiments were carried out to evaluate the effect of bioemulsificant exopolysaccharide (EPS₂₀₀₃) on microbial community dynamics. An experimental seawater microcosm, supplemented with crude oil and EPS₂₀₀₃ (SW+OIL+EPS₂₀₀₃), was monitored for 15 days and compared to control microcosm (only oil-polluted seawater, SW+OIL). Determination of bacterial abundance, heterotrophic cultivable and hydrocarbon-degrading bacteria were carried out during all experimentation period. The microbial community dynamic was monitored by isolation of total RNA, RT-PCR amplification of 16S rRNA, cloning and sequencing. Oil degradation was monitored by GC-MS analysis. Bioemulsificant addition stimulated an increase of the total bacterial abundance, change in the community structure and activity. The bioemulsificant also increased of 5 times the oil biodegradation rate. The data obtained from microcosm experiment indicated that EPS₂₀₀₃ could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.

journal_name

Mar Pollut Bull

authors

Cappello S,Genovese M,Della Torre C,Crisari A,Hassanshahian M,Santisi S,Calogero R,Yakimov MM

doi

10.1016/j.marpolbul.2012.07.046

subject

Has Abstract

pub_date

2012-12-01 00:00:00

pages

2820-8

issue

12

eissn

0025-326X

issn

1879-3363

pii

S0025-326X(12)00367-0

journal_volume

64

pub_type

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