Abstract:
OBJECTIVE:Accumulating evidence suggests that long non-coding RNAs (lncRNAs) are playing critical roles in tumorigenesis. The present study aimed to investigate the expression pattern and effects of lncRNA DSCAM-AS1 (DSCAM-AS1) that was a newly discovered lncRNA in melanoma. PATIENTS AND METHODS:Real-time quantitative PCR (polymerase chain reaction) was performed to determine the expression of DSCAM-AS1 in melanoma tissues and cell lines. Kaplan-Meier and Cox regression analyses were utilized to assess the association between the DSCAM-AS1 and overall survival of patients in melanoma patients. The CCK-8 assay, colony formation assay, flow cytometry assays, transwell and wound scratch assays were performed to determine the biological function of DSCAM-AS1 in tumor cells behaviors. Then, DSCAM-AS1-specific miRNA was further confirmed using the dual-luciferase reporter assay and Western blotting. RESULTS:In this research, we showed that the expression of DSCAM-AS1 was significantly upregulated in melanoma samples and cell lines. Clinical investigation indicated that higher expression of DSCAM-AS1 was associated with ulceration and advanced stage and led to significantly poorer survival time. High DSCAM-AS1 expression in melanoma was confirmed to be an independent predictor of poor survival of patients using univariate and multivariate analysis. Functional investigations revealed that knockdown of DSCAM-AS1 inhibited the ability of cell proliferation, colony formation, migration, invasion, whereas promoted cell apoptosis. Furthermore, mechanistic investigations indicated that DSCAM-AS1 could interact with miR-136 and negatively influence the expression of miR-136. CONCLUSIONS:Our findings showed that DSCAM-AS1 is a novel tumor-related molecule involved in melanoma progression as well as a potential prognostic biomarker and therapeutic target.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Huang YL,Xu Q,Wang Xdoi
10.26355/eurrev_201904_17567subject
Has Abstractpub_date
2019-04-01 00:00:00pages
2888-2897issue
7eissn
1128-3602issn
2284-0729journal_volume
23pub_type
杂志文章abstract:OBJECTIVE:This study aims to explore the ocular surface of late stage eye burns by impression cytology (IC) and analyze the cytological changes and their relationship to ocular surface abnormalities. PATIENTS AND METHODS:68 eyes with late stage eye burns (thermal burn: 28 eyes; alkali burn: 26 eyes; acid burn: 14 eyes...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00
abstract::Intrahepatic cholangiocarcinoma (ICC) is a rare malignant tumor of the biliary tract with growing incidence and dismal prognosis. It is responsible for 10-20% of primary liver cancer worldwide, but early diagnosis is still a challenge and few treatment options are available. Aim of this review is to summarize the curr...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2017-02-01 00:00:00
abstract:INTRODUCTION:In the latest years the use of lung ultrasound is increasing in the evaluation of pleural effusions, because it makes follow-up easier and drainage more efficient by providing guidance on the most appropriate sampling site. However, no standardized approach for ultrasound-guided thoracenthesis is actually ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the effect of 5-fluorouracil mobilized bone marrow regenerative cells (BMRCs) transplantation on brain injury following focal cerebral ischemia and to explore the mechanisms. MATERIALS AND METHODS:Male Sprague-Dawley rats were subjected to middle cerebral artery occlusio...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,收录出版
doi:
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVE:Readmissions to hospital after discharge are considered adverse, serious and costly outcomes. In the last years, two new scores have been proposed to identify patients at high risk of hospital readmission, the HOSPITAL and the Elders Risk Assessment (ERA) indexes. The aim of this study was to evaluate these t...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-11-01 00:00:00
abstract:OBJECTIVE:We aimed to investigate whether miR-181d may be involved in steroid-induced osteonecrosis of the femoral head (ONFH) and its underlying mechanism. PATIENTS AND METHODS:Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was used to detect the expression of miR-181d in bone marrow of 5 case...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201807_15393
更新日期:2018-07-01 00:00:00
abstract::Cryptorchidism is the most frequent defect of the male urogenital tract at birth. It represents a risk factor for primitive testiculopathy associated with long-term complications (infertility, testicular neoplasia, and hormonal changes). An only consensus exists: "children with bilateral cryptorchidism who are not tre...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2009-09-01 00:00:00
abstract:OBJECTIVE:To explore the influence of butorphanol on myocardial ischemia/reperfusion (I/R) injury in rats through the mitogen-activated protein kinase (MAPK) signaling pathway. MATERIALS AND METHODS:The I/R model in Sprague-Dawley rats was established. The rats were randomly divided into normal group (n=20), myocardia...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201912_19695
更新日期:2019-12-01 00:00:00
abstract:UNLABELLED:OBJECTIVES AND MATERIALS AND METHODS: Medicinal plants constitute an important source of potential therapeutic agents for diabetes and hyperlipidemia. The purpose of present study was to investigate the effect of leaf extract of Moringa oleifera on inhibition of alpha-glucosidase and pancreatic alpha-amylase...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2011-07-01 00:00:00
abstract::Drug-induced liver diseases (DILD) are clinico-pathologic patterns of liver injury caused by drugs or other foreign compounds. Steatohepatitis is a rare form of DILD, and drugs account for fewer than 2% of non-alcoholic steatohepatitis (NASH). Drugs known to be capable of inducing steatosis and steatohepatitis can be ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2005-09-01 00:00:00
abstract::Novel coronavirus disease-19 (COVID-19) has widely spread all over the world and seriously threatened people's health. This disease is currently diagnosed by clinical features, chest computed tomography (CT) scan, and nucleic acid test of severe acute respiratory syndrome coronavirus (SARS-CoV-2). Recently, some studi...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202006_21709
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVE:Metabolic syndrome is characterized by abdominal obesity, hypertriglyceridemia and hyperglycemia. Fatty acid binding protein 4 (FABP4), as a member of intracellular lipid chaperones, is not only engaged in lipid transport but involved in inflammation and insulin resistance. The present study was to investigat...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202012_24182
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVE:In this study, we investigated whether certain types of lipid profiles are major contributors of acute ischemic stroke (AIS). PATIENTS AND METHODS:We screened 13,285 hospitalized patients in two stroke medical centers treated with thrombolysis, thrombectomy, or conventional care for anterior cerebral artery-...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202002_20209
更新日期:2020-02-01 00:00:00
abstract:OBJECTIVE:We evaluated the effect of different concentrations of the esterase inhibitor, AEBSF, and acid treatment on acyl-ghrelin stability in human plasma samples subjected to a freeze/thaw cycle. MATERIALS AND METHODS:Four plasma samples were collected from each donor and treated with the following concentrations o...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202002_20216
更新日期:2020-02-01 00:00:00
abstract:OBJECTIVE:The X-linked inhibitor of apoptosis protein (XIAP) is associated with the development of various tumors. The abnormal miR-142 expression is associated with the onset of lung cancer. Bioinformatics analysis revealed a targeted relationship between miR-142 and XIAP. This report investigated whether miR-142 play...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201909_18852
更新日期:2019-09-01 00:00:00
abstract:OBJECTIVE:To investigate the characteristic changes in serum estradiol and leptin levels in patients with cerebral-cardiac syndrome (CCS) induced by subarachnoid hemorrhage (SAH). PATIENTS AND METHODS:Ninety-six female patients with early stage of SAH (within 48 h of onset), who were admitted in our department between...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:To elucidate whether long non-coding RNA (lncRNA) Bmncr could inhibit RANML-induced osteoclast differentiation, thus alleviating the progression of osteoporosis. MATERIALS AND METHODS:Expression level of lncRNA Bmncr at different stages of osteoclast differentiation was detected by quantitative Real Time-Pol...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201911_19411
更新日期:2019-11-01 00:00:00
abstract:OBJECTIVE:Clubfoot is a complex congenital three-dimensional foot deformity, which affects 150,000-200,000 newborn babies annually around the world. A good understanding of the alignment of the two osseous columns and the lower leg of the ankle and foot complex is essential for evaluating the severity of clubfoot. The ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201903_17225
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVE:To evaluate the recurrent ischemic events and risk factors in patients with symptomatic intracranial artery stenosis. PATIENTS AND METHODS:Patients with acute cerebral infarction or transient ischemia attach (TIA) and intracranial arterial stenosis confirmed through CTA examination, were enrolled from the De...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
abstract::Prurigo nodularis of Hyde is a skin disorder characterized by pruritic excoriated nodules. Improvement in pruritus and decrease in nodules was demonstrated in patients treated with oral thalidomide 200-400 mg daily. We report a case of a 52 year old woman with a history of widespread, persistent, and intensely pruriti...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2009-03-01 00:00:00
abstract:OBJECTIVE:Synthetic cathinones are an emerging class of designer drugs abused of due to their psychostimulant and hallucinogenic effects, similar to those of cocaine, methylenedioxymethamphetamine (MDMA), amphetamines and methamphetamines. Mephedrone is a cathinone analogue (4-methyl aromatic analogue of methcathinone)...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2015-10-01 00:00:00
abstract::Internal abdominal hernias are a rare cause of intestinal obstruction (0.2-0.9%). Transmesenteric hernia is a rare type of internal hernia and usually in adult people is acquired. We report the case of a 44 year-old caucasian female with a small bowel occlusion after right nephrectomy for clear cell renal carcinoma ca...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2013-03-01 00:00:00
abstract:OBJECTIVE:To study the mechanism of lncRNA GAS5 affecting epithelial-mesenchymal transition and invasion of breast cancer cells by regulating miR-216b. PATIENTS AND METHODS:Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was used to detect the expressions of GAS5 and miR-216b in breast cancer and paracancer...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202005_21176
更新日期:2020-05-01 00:00:00
abstract:OBJECTIVE:Cervical cancer rate is increasing recently. LncRNA UCA1 plays a role in gynecological tumors, but its expression and mechanism in cervical cancer have not yet been elucidated. PATIENTS AND METHODS:The tumor tissues and adjacent tissues of cervical cancer patients were collected to measure LncRNA UCA1 and mi...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202010_23197
更新日期:2020-10-01 00:00:00
abstract:OBJECTIVE:Recently, the role of long noncoding RNA (lncRNAs) in tumor progression has attracted much attention. The aim of this study was to investigate the role of lncRNA SNHG16 in the development of Wilms' tumor, and to explore the underlying mechanism. PATIENTS AND METHODS:Real Time-quantitative Polymerase Chain Re...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,收录出版
doi:10.26355/eurrev_202004_20994
更新日期:2020-04-01 00:00:00
abstract::This short review describes the conceptual development in the search for the enteric neural circuits with the initial identifications of the classes of enteric neurons on the bases of their morphology, neurochemistry, biophysical properties, projections and connectivity. The discovery of the presence of multiple neuro...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2008-08-01 00:00:00
abstract:OBJECTIVE:To investigate the effects of miR-32-5p on the biological behaviors of cervical cancer (CCa), the relevant mechanism was studied in CCa cell lines (HeLa) in vitro. PATIENTS AND METHODS:The expression level of miR-32-5p was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Ta...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201901_16752
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVE:To study the expression level of the long non-coding ribonucleic acid (lncRNA) KCNQ1 overlapping transcript 1 (KCNQ1OT1) in the patients with myelodysplastic syndrome (MDS), as well as the correlation between the expression level of lncRNA KCNQ1OT1 and the clinical diagnosis and prognosis of MDS. PATIENTS AN...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202005_21341
更新日期:2020-05-01 00:00:00
abstract:OBJECTIVE:Pathogenesis factor of pregnant hypertension is still unclear and lacks of effective treatment. MiR-155 is a recently discovered miRNA molecule with differential expression in pregnant hypertension, which participates in the disease regulation. As a downstream target gene of miR-155, FOXO3a is correlated with...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201810_16133
更新日期:2018-10-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to improve the understanding of FBA in children and to decrease the rate of misdiagnosis, missed diagnosis and morbidity. PATIENTS AND METHODS:We analyzed the clinical features and the three-dimensional reconstructed CT images of 590 children with foreign body aspiration (FBA) in th...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00