当前位置： SCI文献检索 > Acta Biomaterialia期刊下所有文献 > Impact of the composition of alginate and gelatin derivatives in bioconjugated hydrogels on the fabrication of cell sheets and spherical tissues with living cell sheaths.

Impact of the composition of alginate and gelatin derivatives in bioconjugated hydrogels on the fabrication of cell sheets and spherical tissues with living cell sheaths.

Abstract：

:Gelatin and alginate derivatives possessing phenolic hydroxyl moieties (gelatin-Ph and Alg-Ph) were dissolved in aqueous solution and conjugated via horseradish peroxidase-catalyzed crosslinking, resulting in hydrogelation. The objective of creating the hydrogels was to prepare cell sheets and spherical tissues wrapped in living cell sheaths. An increase in the gelatin-Ph content in the hydrogel improved cellular adhesion on the hydrogel surface but hindered degradability by alginate lyase. A hydrogel with the desired characteristics was obtained from a solution containing 0.5% (w/v) gelatin-Ph and 1.5% (w/v) Alg-Ph. Human aortic endothelial (HAE) cells and mouse embryo fibroblast 10T1/2 cells grew on the hydrogels and could be harvested as cell sheets by treatment with alginate lyase. 10T1/2 cells enclosed in Alg-Ph/gelatin-Ph microcapsules composed of the conjugate hydrogel elongated on the inner surface of the microcapsules and grew three times faster than those enclosed in Alg-Ph microcapsules. Alg-Ph/gelatin-Ph microcapsules not only supported growth of the enclosed cells into spherical tissues, but also provided a cell adhesive outer surface for the fabrication of an HAE cell layer. Finally, spherical tissues of 10T1/2 cells wrapped in living HAE cell sheaths were obtained by treatment with alginate lyase.

journal_name

Acta Biomater

journal_title

Acta biomaterialia

authors

Liu Y,Sakai S,Taya M

doi

10.1016/j.actbio.2013.01.037

subject

Has Abstract

pub_date

2013-05-01 00:00:00

pages

6616-23

issue

5

eissn

1742-7061

issn

1878-7568

pii

S1742-7061(13)00052-4

journal_volume

9

pub_type

杂志文章

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