Abstract:
:Neoparamoeba perurans is the aetiological agent of amoebic gill disease (AGD) in salmonids, however multiple other amoeba species colonise the gills and their role in AGD is unknown. Taxonomic assessments of these accompanying amoebae on AGD-affected salmon have previously been based on gross morphology alone. The aim of the present study was to document the diversity of amoebae colonising the gills of AGD-affected farmed Atlantic salmon using a combination of morphological and sequence-based taxonomic methods. Amoebae were characterised morphologically via light microscopy and transmission electron microscopy, and by phylogenetic analyses based on the 18S rRNA gene and cytochrome oxidase subunit I (COI) gene. In addition to N. perurans, 11 other amoebozoans were isolated from the gills, and were classified within the genera Neoparamoeba, Paramoeba, Vexillifera, Pseudoparamoeba, Vannella and Nolandella. In some cases, such as Paramoeba eilhardi, this is the first time this species has been isolated from the gills of teleost fish. Furthermore, sequencing of both the 18S rRNA and COI gene revealed significant genetic variation within genera. We highlight that there is a far greater diversity of amoebae colonising AGD-affected gills than previously established.
journal_name
Eur J Protistoljournal_title
European journal of protistologyauthors
English CJ,Tyml T,Botwright NA,Barnes AC,Wynne JW,Lima PC,Cook MTdoi
10.1016/j.ejop.2018.10.003subject
Has Abstractpub_date
2019-02-01 00:00:00pages
27-45eissn
0932-4739issn
1618-0429pii
S0932-4739(18)30087-7journal_volume
67pub_type
杂志文章abstract::A strain of the free-living flagellate Dimastigella trypaniformis Sandon was isolated from the gut contents of the Australian termite Mastotermes darwiniensis Froggatt. The fine structure of the isolate (Ulm strain) was compared with a strain isolated from soil in Scotland (Glasgow strain). The biflagellated trophozoi...
journal_title:European journal of protistology
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journal_title:European journal of protistology
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journal_title:European journal of protistology
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journal_title:European journal of protistology
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