Abstract：
:Toxoplasma gondii is a zoonotic parasite with a world-wide distribution. House mice (Mus musculus) play an important role as a reservoir host in the parasite life cycle. However, their detection in mouse brain is limited because the host potentially harbours only a few tissue cysts. In order to improve the diagnosis, we tested a novel protocol for T. gondii detection in mice and compared this technique to a standard PCR-based protocol using a commercial kit for DNA isolation. Efficacy of magnetic capture for isolation of T. gondii DNA from whole host brains was tested in brain samples of laboratory mice spiked with 1 up to 10(4) tachyzoites. Real-time PCR revealed that even 1-5 tachyzoites can be detected after magnetic capture. Also this method is suitable to quantify parasite numbers in mouse brains with more than 10 tachyzoite equivalents. To assess the two techniques in wild mice, we employed a dataset consisting of 243 individuals. The prevalence of T. gondii detected by magnetic capture and qPCR and by commercial isolation and PCR was 1.2% and 0%, respectively. The magnetic capture and quantitative PCR seems to be a highly sensitive and specific diagnostic method for both laboratory research and wild population surveys.
journal_name
Eur J Protistoljournal_title
European journal of protistologyauthors
Juránková J,Hůrková-Hofmannová L,Volf J,Baláž V,Piálek Jdoi
10.1016/j.ejop.2013.08.002subject
Has Abstractpub_date
2014-02-01 00:00:00pages
11-5issue
1eissn
0932-4739issn
1618-0429pii
S0932-4739(13)00069-2journal_volume
50pub_type
杂志文章abstract：:This review gives a brief report on some recent advances in the study of molecular biology of ciliate genes. The main emphasis will be put on those results which may contribute to a better understanding of how gene structure and genome organization has evolved in ciliates. New data will be discussed in relation to the...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/S0932-4739(11)80384-6
更新日期：1990-08-31 00:00:00
abstract：:The behavior of Euplotes crassus has been studied qualitatively and quantitatively in an attempt of throwing new light onto the problem of the relationships occurring among motor behavior, systematic position and evolutionary strategies of the ciliated protozoa. It was found that both single and paired Euplotes creep ...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/S0932-4739(88)80056-7
更新日期：1988-03-01 00:00:00
abstract：:Since its description 35 years ago from Quebec, Canada, the poorly known monospecific testate rhizopod genus Puytoracia (type species, Puytoracia bergeri) has been reported only from the Himalayan region of Nepal. This species was recently found at 13 locations in Ontario, Canada, and the range of form variation of th...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/j.ejop.2006.04.002
更新日期：2006-09-01 00:00:00
abstract：:We report the use of nile red as a rapid and inexpensive method to estimate cellular lipids in three species of Paramecium and in Tetrahymena by the direct application of the dye to living or fixed cells without extraction and purification. Qualitative estimates of the relative changes in the lipid content of cells of...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/S0932-4739(11)80129-X
更新日期：1990-06-29 00:00:00
abstract：:The apostome ciliate Hyalophysa chattoni, a symbiont of the estuarine grass shrimp Palaemonetes pugio, was tested for its growth and reproductive ability in a wide range of salinities from 0.1 to 55 ppt. Shrimp, with their attached ciliates, were slowly acclimated to different salinities in order to assess protozoan c...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/j.ejop.2007.11.002
更新日期：2008-05-01 00:00:00
abstract：:The effects of 0.2-2.0 mM PtCl(4) have been investigated, the purpose was to test whether the compound would affect Tetrahymena in a manner similar to that of cis-dichlorodiammine-platinum(II), or cisplatin, which in 0.83 mM inhibits cell proliferation for 24 h and induces diminution of the inner mitochondrial membran...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/S0932-4739(11)80044-1
更新日期：1992-05-22 00:00:00
abstract：:Acanthamoebae feed on bacteria but are also frequent hosts of bacterial symbionts. Here, we describe the stable co-occurrence of two symbionts, one affiliated to the genus Parachlamydia and the other to the candidate genus Paracaedibacter (Alphaproteobacteria), within a clinical isolate of Acanthamoeba hatchetti genot...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/j.ejop.2016.04.002
更新日期：2016-10-01 00:00:00
abstract：:The morphology and infraciliature of two pleurostomatid ciliates, Amphileptus aeschtae nov. spec. and Amphileptus eigneri nov. spec., collected from shrimp-culturing ponds of the Bohai Sea, China, were investigated using live observations and the protargol impregnation method. The new species A. aeschtae is identified...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/j.ejop.2006.10.002
更新日期：2007-06-01 00:00:00
abstract：:A new species of unicellular opisthokont protist, Nuclearia thermophila sp. nov., was isolated from the warm spring water of Yunoko Lake, Japan, and has been described using light and electron microscopy. It exists as a spherical floating form and a flattened amoeboid form showing various shapes. The cells occasionall...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/j.ejop.2008.09.004
更新日期：2009-05-01 00:00:00
abstract：:Neoparamoeba perurans is the aetiological agent of amoebic gill disease (AGD) in salmonids, however multiple other amoeba species colonise the gills and their role in AGD is unknown. Taxonomic assessments of these accompanying amoebae on AGD-affected salmon have previously been based on gross morphology alone. The aim...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/j.ejop.2018.10.003
更新日期：2019-02-01 00:00:00
abstract：:We have characterized the intracellular development and ultrastructure of a novel parasite that infected the marine benthic dinoflagellate Prorocentrum fukuyoi. The parasite possessed a combination of features described for perkinsids and syndineans, and also possessed novel characters associated with its parasitic li...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/j.ejop.2007.08.004
更新日期：2008-02-01 00:00:00
abstract：:A new colpodid ciliate, Bresslauides pratensis n. sp., was discovered in soil from a meadow field in Turkey. Its morphology was investigated using live observation, protargol impregnation, silver nitrate impregnation, and scanning electron microscopy. The new species differs from the congeners by the following combina...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/j.ejop.2012.01.006
更新日期：2012-08-01 00:00:00
abstract：:The morphology and the infraciliature of Pseudokeronopsis rubra [7, 10, 11], P. carnea [8] nov. comb, and P. flava [8] nov. comb, are redescribed using protargol silver staining, biometry, and chromatography. These species differ in the colour and composition of the pigment granules, the length of the midventral rows,...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/S0932-4739(87)80010-X
更新日期：1987-11-01 00:00:00
abstract：:The infraciliature and morphogenesis of Paraholosticha sterkii (Garnjobst, 1934) n. comb. (Basionym: Stylonethes sterkii) are described. Before division a cell encysts and after division each tomite forms an individual cyst within the common one. The ciliates cease to move and the kinetome is resorbed. With the beginn...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/S0932-4739(88)80038-5
更新日期：1988-07-01 00:00:00
abstract：:Resting cysts of Parentocirrus hortualis were investigated, using live observation, SEM and TEM. Processes during encystation and excystation were observed in vivo under the light microscope. During encystation, the trophic body becomes globular, the ciliature is resorbed in an anterior direction, the macronuclear nod...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/j.ejop.2015.12.003
更新日期：2016-04-01 00:00:00
abstract：:In the absence of distinct morphological characteristics, knowledge of genetic relationships within and between protist parasite species is important for determining reservoir hosts and understanding the biology of the causative agents of emerging diseases. The genus Hematodinium is a member of Syndinea, an ubiquitous...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/j.ejop.2009.10.002
更新日期：2010-01-01 00:00:00
abstract：:Hyalophysa clampi Browning and Landers, 2012 was reexamined to determine all stages in the life cycle of this symbiotic ciliate. The cell feeds as a normal exuviotroph within the exoskeleton of its molted crayfish host but does not encyst following the trophont stage. Trophonts transform into swimming tomont stages, w...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/j.ejop.2019.125654
更新日期：2020-02-01 00:00:00
abstract：:Gravitaxis of Paramecium at different oxygen concentrations is analyzed by means of a computer controlled image-analysis system. The gravitactic response depends on the oxygen tension: at low-oxic conditions Paramecium shows a precise negative gravitaxis which changes into a random distribution if the oxygen tension i...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/S0932-4739(11)80065-9
更新日期：1991-09-09 00:00:00
abstract：:The route of infection of two endonucleobiotic bacteria in Paramecium caudatum was investigated with the electron microscope. The bacterium Holospora elegans specifically infects the micronucleus, whereas Holospora obtusa infects the macronucleus. The infectious forms of both bacteria are ingested by the prospective h...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/S0932-4739(89)80037-9
更新日期：1989-02-24 00:00:00
abstract：:Nuclear phenomena at conjugation of the multimicronucleate ciliate Blepharisma japonicum were investigated by using fluorescence microscopy. Several hours after cells united in pairs, micronuclei differentiated into meiotic micronuclei and "somatomicronuclei". Meiotic micro-nuclei participated in conventional sexual p...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/S0932-4739(11)80340-8
更新日期：1991-06-21 00:00:00
abstract：:The living morphology, infraciliature and silverline system of two small marine Euplotes species, E. orientalis spec. nov. and E. raikoviAgamaliev, 1966, isolated from a sandy beach near Qingdao, China, were investigated. Euplotes orientalis is characterized by a combination of features including their small size (35-...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/j.ejop.2009.11.003
更新日期：2010-05-01 00:00:00
abstract：:In a study on ciliate diversity, we discovered the new hypotrich species, Gonostomum jangbogoensis n. sp., in freshwater from Terra Nova Bay, Victoria Land, southeast Antarctica. We describe its morphology and morphogenesis using standard methods, and the SSU rRNA gene phylogeny is provided as well. Morphology of Gono...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/j.ejop.2019.125669
更新日期：2020-04-01 00:00:00
abstract：:Five scuticociliates, collected from China, were morphologically studied using standard methods One represents a new member of the genus Falcicyclidium, F. citriforme nov. spec., which can be recognised mainly by a combination of the following characters: usually two macronuclear nodules, buccal field about half of bo...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/j.ejop.2017.03.003
更新日期：2017-06-01 00:00:00
abstract：:Planktonic testate amoebae in floodplains exhibit a broad-range of morphological variability. The variation size is already known, but it is necessary to know how this is for morphological variables. This study aimed to identify the relationships between testate amoebae morphology and environmental factors in four neo...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/j.ejop.2016.08.006
更新日期：2016-10-01 00:00:00
abstract：:The free-living anaerobic flagellate Psalteriomonas vulgaris n. spec. is described. The organism has four flagella of equal length which arise immediately subapically to the anterior part of the cell, within the apex of the ventral groove. The ultrastructural organization of the mastigont system and the ventral groove...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/S0932-4739(11)80302-0
更新日期：1993-02-19 00:00:00
abstract：:Two new marine scuticociliates, Sathrophilus planus n. sp. and Pseudoplatynematum dengi n. sp., isolated from coastal waters of Qingdao, northern China, were investigated using live observation and silver impregnations. Sathrophilus planus can be recognized by its elongate and conspicuously flattened body, ca. 16 soma...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/j.ejop.2010.03.002
更新日期：2010-08-01 00:00:00
abstract：:More than 1000 secretory organelles (trichocysts) are docked at the plasma membrane of Paramecium cells. After stimulation, the trichocyst contents are expelled as needle-like structures in an exocytotic response. Neither the function nor the natural stimulus of trichocyst exocytosis are known in this well established...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/S0932-4739(11)80256-7
更新日期：1991-11-29 00:00:00
abstract：:Prorodon spirogyrophagus nov. spec. is described by observations on living and silver-stained specimens. Important morphometric data are presented and the feeding habits are described. The new species is characterized by a body size of about 450 × 200 μm, numerous independently functioning contractile vacuoles each wi...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/S0932-4739(89)80009-4
更新日期：1989-07-07 00:00:00
abstract：:Cell division in Prorocentrum micans was studied with TEM, SEM and with fluorescence microscope techniques to visualize cytoskeletal structures. The cleavage furrow consists of the plasma membrane and two valvar vesicles. Its leading edge is lined with actin filaments. The completion of cytokinesis (like in some other...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/S0932-4739(11)80175-6
更新日期：1990-03-09 00:00:00
abstract：:The marine dinoflagellate Cochlodinium polykrikoides is a harmful algal bloom (HAB) species that severely impacts the environment and causes huge economic losses. Yellow clay (YC), considered to be a non-toxic and naturally-occurring material, represents an important step towards the direct control of HABs. In the pre...
journal_title：European journal of protistology
pub_type： 杂志文章
doi：10.1016/j.ejop.2019.125642
更新日期：2019-10-01 00:00:00
