Abstract:
:We report the use of nile red as a rapid and inexpensive method to estimate cellular lipids in three species of Paramecium and in Tetrahymena by the direct application of the dye to living or fixed cells without extraction and purification. Qualitative estimates of the relative changes in the lipid content of cells of varying culture ages were obtained using fluorescence microscopy, while semiquantitative determinations were obtained by measuring the total fluorescence from the emission spectrum (excitation, 535 nm) of fixed cells treated with excess nile red. The relative amounts of neutral (excitation, 488 nm; emission, 540 nm) and polar (excitation, 535 nm; emission, 680 nm) lipids were approximated using fluorescence intensity at these selected spectral conditions to avoid any spill over from each other. The patterns of change with culture age in total lipids in Tetrahymena and in total, neutral and polar lipids in Paramecium obtained using nile red agreed well with published gravimetric data for these ciliates.
journal_name
Eur J Protistoljournal_title
European journal of protistologyauthors
Cole TA,Fok AK,Ueno MS,Allen RDdoi
10.1016/S0932-4739(11)80129-Xsubject
Has Abstractpub_date
1990-06-29 00:00:00pages
361-8issue
4eissn
0932-4739issn
1618-0429pii
S0932-4739(11)80129-Xjournal_volume
25pub_type
杂志文章abstract::The ultrastructural cytology and reproduction of the hyperparasitic microsporidium Amphiamblys capitellides (Caullery and Mesnil, 1897) is described. Merogonial reproduction was not observed. The sporogony comprises two sequences: a sac-bound sporogony in close contact with the cytoplasm of the host and a free sporogo...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2006.07.001
更新日期:2006-11-01 00:00:00
abstract::Acanthamoebae feed on bacteria but are also frequent hosts of bacterial symbionts. Here, we describe the stable co-occurrence of two symbionts, one affiliated to the genus Parachlamydia and the other to the candidate genus Paracaedibacter (Alphaproteobacteria), within a clinical isolate of Acanthamoeba hatchetti genot...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2016.04.002
更新日期:2016-10-01 00:00:00
abstract::The living morphology, infraciliature and silverline system of two small marine Euplotes species, E. orientalis spec. nov. and E. raikoviAgamaliev, 1966, isolated from a sandy beach near Qingdao, China, were investigated. Euplotes orientalis is characterized by a combination of features including their small size (35-...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2009.11.003
更新日期:2010-05-01 00:00:00
abstract::This paper summarises the Symposium 'Concepts in Protistology', during the VI European Congress of Protistology, Berlin, 25-29 July 2011. There is an increasing focus on cataloguing the number of species on earth, species barcoding initiatives, and the increasing need to reconcile molecular with morphological data in ...
journal_title:European journal of protistology
pub_type:
doi:10.1016/j.ejop.2011.11.004
更新日期:2012-05-01 00:00:00
abstract::The species Leptomyxa flabellata was described by Goodey in 1915 and re-isolated by Pussard and Pons in 1976. It seems that it was never seen (or never recognized) again since that time. The strain designated as "Leptomyxa flabellata CCAP 1546/2" was studied by Cann in 1984, however the quality of the electron microsc...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2017.12.001
更新日期:2018-02-01 00:00:00
abstract::The free-living ciliates of the order Metopida Jankowski, 1980 are pivotal players in the microbial food web of the sulfuretum, acting as hosts to prokaryotic endo- and ectosymbionts. They are also of interest in the study of the function and evolution of their mitochondrion-related organelle, the hydrogenosome. The t...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2017.07.003
更新日期:2017-10-01 00:00:00
abstract::Expression of surface antigens is usually mutually exclusive, meaning that only one protein is present on the cell surface. With the RNAi feeding technology we induce serotype shifts in Paramecium tetraurelia which are demonstrated to be incomplete, meaning that the cells remain in a shifting state. The coexpression o...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2005.09.003
更新日期:2006-03-01 00:00:00
abstract::This review gives a brief report on some recent advances in the study of molecular biology of ciliate genes. The main emphasis will be put on those results which may contribute to a better understanding of how gene structure and genome organization has evolved in ciliates. New data will be discussed in relation to the...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(11)80384-6
更新日期:1990-08-31 00:00:00
abstract::Protistan parasites have an undisputed global health impact. However, outside of a few key exceptions, e.g. the agent of malaria, most of these infectious agents are neglected as important health threats. The Symposium entitled "Free-living amoebae and neglected pathogenic protozoa: health emergency signals?" held at ...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2020.125760
更新日期:2020-11-28 00:00:00
abstract::Licnophora chattoni, found in association with Zyzzyzus warreni, a tubulariid hydroid epizoic in sponges from São Sebastião (SP, Brazil), is redescribed and illustrated using light and electron microscopy. The ciliate has a flexible, transparent body formed by an oval anterior region linked to the posterior basal disc...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2011.07.004
更新日期:2012-01-01 00:00:00
abstract::Lake Tanganyika is world famous for a fauna that is rich in endemic species. On the other hand, it seems to be particularly poor in species of planktonic ciliates. Only the coastal infusorial fauna, for the most part mesopsammic, constitutes an extensive biomass with relatively few species. However, some are character...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(11)80144-6
更新日期:1991-02-22 00:00:00
abstract::Microbial eukaryotes living in low oxygen environments often have novel physiological and morphological features that facilitate symbiotic relationships with bacteria and other means for acquiring nutrients. Comparative studies of these features provide evidence for phylogenetic relationships and evolutionary history....
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2012.07.001
更新日期:2013-01-01 00:00:00
abstract::The morphology and morphogenesis of a marine ciliate, Epiclintes auricularis rarisetus nov. sspec., collected from Qingdao, northern China were studied on live and protargol-impregnated specimens. This isolate can be recognized by its elongate, contractile and tripartite body with a size of 200-400 x 20-40microm in vi...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2009.01.005
更新日期:2009-11-01 00:00:00
abstract::Gravitaxis of Paramecium at different oxygen concentrations is analyzed by means of a computer controlled image-analysis system. The gravitactic response depends on the oxygen tension: at low-oxic conditions Paramecium shows a precise negative gravitaxis which changes into a random distribution if the oxygen tension i...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(11)80065-9
更新日期:1991-09-09 00:00:00
abstract::I discuss how different feeding modes and related cellular structures map onto the eukaryote evolutionary tree. Centrally important for understanding eukaryotic cell diversity are Loukozoa: ancestrally biciliate phagotrophic protozoa possessing a posterior cilium and ventral feeding groove into which ciliary currents ...
journal_title:European journal of protistology
pub_type: 杂志文章,评审
doi:10.1016/j.ejop.2012.06.001
更新日期:2013-05-01 00:00:00
abstract::Haploid micronuclei produced by meiosis in Paramecium caudatum are destined either to survive or to degenerate depending upon their position in the cytoplasm. The behavior of the four meiotic division products in living cells and their DNA synthesis patterns were examined to determine whether there were differences be...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(11)80317-2
更新日期:1992-02-21 00:00:00
abstract::We observed reproductive swarmer cells of the nassellarian and spumellarian polycystine radiolarians Didymocyrtis ceratospyris, Pterocanium praetextum, Tetrapyle sp., and Triastrum aurivillii using light, scanning and transmission electron microscopy. The swarmer cells had subspherical to ovoid or spindle shapes with ...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2016.02.007
更新日期:2016-06-01 00:00:00
abstract::The present study was undertaken to describe Cryptosporidium spp. infection in tree squirrels from 17 locations in Northern Italy. A total of 357 squirrels were examined, including species native to Europe (Sciurus vulgaris; n=123), and species introduced from North America (Sciurus carolinensis; n=162) and Southeast ...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2017.09.007
更新日期:2017-10-01 00:00:00
abstract::The infraciliature and morphogenesis of Paraholosticha sterkii (Garnjobst, 1934) n. comb. (Basionym: Stylonethes sterkii) are described. Before division a cell encysts and after division each tomite forms an individual cyst within the common one. The ciliates cease to move and the kinetome is resorbed. With the beginn...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(88)80038-5
更新日期:1988-07-01 00:00:00
abstract::Dileptid and tracheliid ciliates have been traditionally classified within the subclass Haptoria of the class Litostomatea. However, their phylogenetic position among haptorians has been controversial and indicated that they may play a key role in understanding litostomatean evolution. In order to reconstruct the evol...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2011.04.006
更新日期:2011-11-01 00:00:00
abstract::Differences in gel electrophoretic mobility were used to compare tubulin subunits from a variety of distantly related ciliate species. The relative electrophoretic mobility of ciliate tubulins is shown to vary depending upon the electrophoretic conditions used to separate them. Tubulin migration is altered by changing...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(11)80271-3
更新日期:1993-05-28 00:00:00
abstract::Toxoplasma gondii is a zoonotic parasite with a world-wide distribution. House mice (Mus musculus) play an important role as a reservoir host in the parasite life cycle. However, their detection in mouse brain is limited because the host potentially harbours only a few tissue cysts. In order to improve the diagnosis, ...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2013.08.002
更新日期:2014-02-01 00:00:00
abstract::The morphology, infraciliature and silverline system of Trachelocerca trepida Kahl, 1928 has been reinvestigated. The species was found in marine water (Yellow Sea) near Shanghai, Eastchina. Stained specimens (protargol) are characterized morphometrically. The shape of the extrusomes (toxicysts) and the structure of o...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(11)80110-0
更新日期:1990-10-19 00:00:00
abstract::The Javan ferret-badger Melogale orientalis (Carnivora: Mustelidae: Helictidinae) is a small carnivore endemic to Indonesia. In the family Mustelidae, 10 Eimeria, 12 Cystoisopora, one Isospora, and one Hammondia species are known, but no eimeriid coccidia has been yet described in the subfamily Helictinidae (ferret ba...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2019.125668
更新日期:2020-04-01 00:00:00
abstract::The living morphology, infraciliature and morphogenesis of a new ciliate, Urosomoida paragiliformis n. sp., collected from saline soil in Weinan, China, were studied in vivo and after protargol staining. Urosomoida paragiliformis n. sp. is characterized as follows: body elongate-elliptical with no tail, flexible and s...
journal_title:European journal of protistology
pub_type: 杂志文章,评审
doi:10.1016/j.ejop.2016.07.005
更新日期:2016-10-01 00:00:00
abstract::During the last decades, the planktonic cyanobacterium Planktothrix rubescens became a dominant primary producer in many deep pre-alpine lakes. While altered physiochemical conditions due to lake warming seem to favour this cyanobacterial species, its dominance is partly attributed to factors conferring grazing resist...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2017.05.009
更新日期:2017-08-01 00:00:00
abstract::Mitotis in the choanoflagellate Monosiga sp. has been studied by electron microscopy for the first time based on serial sections of metaphase and anaphase. The dense metaphase plate is formed by chromosomes at the centre of the nucleus. Spindle poles are located at the opposite sides of this plate. The nuclear envelop...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(11)80292-0
更新日期:1993-02-19 00:00:00
abstract::The endonucleobiotic bacterium Holospora elegans inhabits the micronucleus of Paramecium caudatum. In this paper the release of the infectious form (IF) of H. elegans from its host is described. During the anaphase of the micronuclear division most of the IFs remain within the connecting piece which, therefore, may be...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(89)80021-5
更新日期:1989-10-27 00:00:00
abstract::The free-living anaerobic flagellate Psalteriomonas vulgaris n. spec. is described. The organism has four flagella of equal length which arise immediately subapically to the anterior part of the cell, within the apex of the ventral groove. The ultrastructural organization of the mastigont system and the ventral groove...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(11)80302-0
更新日期:1993-02-19 00:00:00
abstract::Fine structure of the membrane system was examined in a large heliozoon, Echinosphaerium akamae, especially by means of negative staining and thin sectioning under the electron microscope. In addition to solitary membranous organelles such as mitochondrion, Golgi apparatus and nuclear envelope, there was found a conti...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(89)80047-1
更新日期:1989-02-24 00:00:00