Abstract:
:Differences in gel electrophoretic mobility were used to compare tubulin subunits from a variety of distantly related ciliate species. The relative electrophoretic mobility of ciliate tubulins is shown to vary depending upon the electrophoretic conditions used to separate them. Tubulin migration is altered by changing the pH of the separating gel: the positions of the tubulin subunits at pH 8.1 are apparently reversed when compared with their migration at pH 9.3. Examination of the tubulin electrophoretic behaviour reveals considerable differences among species of ciliates belonging to different taxonomic groups. Such an electrophoretic diversity is in good agreement with the large evolutionary distances within the phylum derived from rRNA sequence analyses. Our results allow us to group the ciliates analyzed in clusters which can be fitted quite satisfactorily into the traditional phylogenetic schemes.
journal_name
Eur J Protistoljournal_title
European journal of protistologyauthors
Delgado P,Fleury Adoi
10.1016/S0932-4739(11)80271-3subject
Has Abstractpub_date
1993-05-28 00:00:00pages
176-81issue
2eissn
0932-4739issn
1618-0429pii
S0932-4739(11)80271-3journal_volume
29pub_type
杂志文章abstract::The morphology and infraciliature of two Frontonia species, F. shii spec. nov. and F. paramagna Chen et al., 2014, isolated from a freshwater pond in northeastern China, were investigated using living observation and silver staining methods Frontonia shii spec. nov. is recognized by the combination of the following ch...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2018.01.002
更新日期:2018-04-01 00:00:00
abstract::Gravitaxis of Paramecium at different oxygen concentrations is analyzed by means of a computer controlled image-analysis system. The gravitactic response depends on the oxygen tension: at low-oxic conditions Paramecium shows a precise negative gravitaxis which changes into a random distribution if the oxygen tension i...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(11)80065-9
更新日期:1991-09-09 00:00:00
abstract::Cell division in Prorocentrum micans was studied with TEM, SEM and with fluorescence microscope techniques to visualize cytoskeletal structures. The cleavage furrow consists of the plasma membrane and two valvar vesicles. Its leading edge is lined with actin filaments. The completion of cytokinesis (like in some other...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(11)80175-6
更新日期:1990-03-09 00:00:00
abstract::The living morphology, infraciliature and silverline system of two small marine Euplotes species, E. orientalis spec. nov. and E. raikoviAgamaliev, 1966, isolated from a sandy beach near Qingdao, China, were investigated. Euplotes orientalis is characterized by a combination of features including their small size (35-...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2009.11.003
更新日期:2010-05-01 00:00:00
abstract::It is well established that in ombrotrophic bogs, water-table depth (WTD) is the primary environmental control on testate amoeba distribution. However, the environmental controls on testate amoebae in minerotrophic fens are less well known and successional change in their assemblages associated with fen-bog peatland d...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2018.07.003
更新日期:2018-10-01 00:00:00
abstract::The ultrastructural cytology and reproduction of the hyperparasitic microsporidium Amphiamblys capitellides (Caullery and Mesnil, 1897) is described. Merogonial reproduction was not observed. The sporogony comprises two sequences: a sac-bound sporogony in close contact with the cytoplasm of the host and a free sporogo...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2006.07.001
更新日期:2006-11-01 00:00:00
abstract::Dileptid and tracheliid ciliates have been traditionally classified within the subclass Haptoria of the class Litostomatea. However, their phylogenetic position among haptorians has been controversial and indicated that they may play a key role in understanding litostomatean evolution. In order to reconstruct the evol...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2011.04.006
更新日期:2011-11-01 00:00:00
abstract::Neoparamoeba perurans is the aetiological agent of amoebic gill disease (AGD) in salmonids, however multiple other amoeba species colonise the gills and their role in AGD is unknown. Taxonomic assessments of these accompanying amoebae on AGD-affected salmon have previously been based on gross morphology alone. The aim...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2018.10.003
更新日期:2019-02-01 00:00:00
abstract::Five scuticociliates, collected from China, were morphologically studied using standard methods One represents a new member of the genus Falcicyclidium, F. citriforme nov. spec., which can be recognised mainly by a combination of the following characters: usually two macronuclear nodules, buccal field about half of bo...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2017.03.003
更新日期:2017-06-01 00:00:00
abstract::During the last decades, the planktonic cyanobacterium Planktothrix rubescens became a dominant primary producer in many deep pre-alpine lakes. While altered physiochemical conditions due to lake warming seem to favour this cyanobacterial species, its dominance is partly attributed to factors conferring grazing resist...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2017.05.009
更新日期:2017-08-01 00:00:00
abstract::The morphology of four cyrtophorian ciliates, Dysteria ovalis, D. semilunaris, Phascolodon vorticella, and Chlamydonellopsis calkinsi, were investigated using living observation and protargol-staining. The morphology of D. ovalis is revealed in detail for the first time, and it is characterized by the following combin...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2016.08.005
更新日期:2016-10-01 00:00:00
abstract::The behavior of Euplotes crassus has been studied qualitatively and quantitatively in an attempt of throwing new light onto the problem of the relationships occurring among motor behavior, systematic position and evolutionary strategies of the ciliated protozoa. It was found that both single and paired Euplotes creep ...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(88)80056-7
更新日期:1988-03-01 00:00:00
abstract::The morphology, ontogenesis, conjugation, and phylogenetic position of Metopus boletus nov. spec. were studied using live observation, various silver impregnation methods, scanning electron microscopy, morphometry, and the 18S rRNA gene sequence. The new species is outstanding in having a mushroom-like appearance; a g...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2019.04.002
更新日期:2019-06-01 00:00:00
abstract::Resting cysts of Parentocirrus hortualis were investigated, using live observation, SEM and TEM. Processes during encystation and excystation were observed in vivo under the light microscope. During encystation, the trophic body becomes globular, the ciliature is resorbed in an anterior direction, the macronuclear nod...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2015.12.003
更新日期:2016-04-01 00:00:00
abstract::Expression of surface antigens is usually mutually exclusive, meaning that only one protein is present on the cell surface. With the RNAi feeding technology we induce serotype shifts in Paramecium tetraurelia which are demonstrated to be incomplete, meaning that the cells remain in a shifting state. The coexpression o...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2005.09.003
更新日期:2006-03-01 00:00:00
abstract::Lake Tanganyika is world famous for a fauna that is rich in endemic species. On the other hand, it seems to be particularly poor in species of planktonic ciliates. Only the coastal infusorial fauna, for the most part mesopsammic, constitutes an extensive biomass with relatively few species. However, some are character...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(11)80144-6
更新日期:1991-02-22 00:00:00
abstract::A new colpodid ciliate, Bresslauides pratensis n. sp., was discovered in soil from a meadow field in Turkey. Its morphology was investigated using live observation, protargol impregnation, silver nitrate impregnation, and scanning electron microscopy. The new species differs from the congeners by the following combina...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2012.01.006
更新日期:2012-08-01 00:00:00
abstract::During conjugation, Stylonychia mytilus undergoes repetitive somatic development by reorganizing the cortical ciliature consecutively for three times. The control of these reorganizations by the nucleus was investigated employing amicronucleates generated by microsurgery. When amicronucleates conjugate, they underwent...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(11)80154-9
更新日期:1991-02-22 00:00:00
abstract::The cortical morphogenesis of Amphisiella australis Blatterer and Foissner, 1988 was investigated using protargol impregnation. In 43% of the examined cells the morphogenesis starts with a dedifferentiation of the two transverse cirri forming the oral primordium. In the other specimens the oral primordium either origi...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(11)80004-0
更新日期:1992-11-20 00:00:00
abstract::The species Leptomyxa flabellata was described by Goodey in 1915 and re-isolated by Pussard and Pons in 1976. It seems that it was never seen (or never recognized) again since that time. The strain designated as "Leptomyxa flabellata CCAP 1546/2" was studied by Cann in 1984, however the quality of the electron microsc...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2017.12.001
更新日期:2018-02-01 00:00:00
abstract::More than 1000 secretory organelles (trichocysts) are docked at the plasma membrane of Paramecium cells. After stimulation, the trichocyst contents are expelled as needle-like structures in an exocytotic response. Neither the function nor the natural stimulus of trichocyst exocytosis are known in this well established...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(11)80256-7
更新日期:1991-11-29 00:00:00
abstract::This review gives a brief report on some recent advances in the study of molecular biology of ciliate genes. The main emphasis will be put on those results which may contribute to a better understanding of how gene structure and genome organization has evolved in ciliates. New data will be discussed in relation to the...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(11)80384-6
更新日期:1990-08-31 00:00:00
abstract::We report the use of nile red as a rapid and inexpensive method to estimate cellular lipids in three species of Paramecium and in Tetrahymena by the direct application of the dye to living or fixed cells without extraction and purification. Qualitative estimates of the relative changes in the lipid content of cells of...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(11)80129-X
更新日期:1990-06-29 00:00:00
abstract::Protistan parasites have an undisputed global health impact. However, outside of a few key exceptions, e.g. the agent of malaria, most of these infectious agents are neglected as important health threats. The Symposium entitled "Free-living amoebae and neglected pathogenic protozoa: health emergency signals?" held at ...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2020.125760
更新日期:2020-11-28 00:00:00
abstract::Mitotis in the choanoflagellate Monosiga sp. has been studied by electron microscopy for the first time based on serial sections of metaphase and anaphase. The dense metaphase plate is formed by chromosomes at the centre of the nucleus. Spindle poles are located at the opposite sides of this plate. The nuclear envelop...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(11)80292-0
更新日期:1993-02-19 00:00:00
abstract::The morphology and the infraciliature of Pseudokeronopsis rubra [7, 10, 11], P. carnea [8] nov. comb, and P. flava [8] nov. comb, are redescribed using protargol silver staining, biometry, and chromatography. These species differ in the colour and composition of the pigment granules, the length of the midventral rows,...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(87)80010-X
更新日期:1987-11-01 00:00:00
abstract::A new ciliate species (Cyclidium porcatum) is the first freshwater anaerobic scuticociliate to be cultured and described. It contains a unique tripartite structure consisting of hydrogenosomes (confirmed by cytochemical staining for hydrogenase), interspersed with methanogens (confirmed by auto fluorescence and in sit...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(11)80281-6
更新日期:1993-05-28 00:00:00
abstract::Copidognathus nautilei Bartsch, 1997, from a hydrothermal vent field of the Mid-Atlantic Ridge at about 13 degrees N, 45 degrees W and 4090 m depth, was infested with the suctorian Corynophrya abyssalis n. sp., with up to 58 epizoans per mite. The new suctorian has a sacciform body with seven longitudinal ribs, a comp...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/j.ejop.2010.02.001
更新日期:2010-08-01 00:00:00
abstract::A new Naegleria species, N. andersoni, is described that occurs world-wide. The new species consists of two subspecies and can be distinguished from previously described species on the basis of antigenic, isoenzyme and restriction enzyme DNA fragment differences and in maximum growth temperature tolerated. N. anderson...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(88)80022-1
更新日期:1988-10-01 00:00:00
abstract::The endonucleobiotic bacterium Holospora elegans inhabits the micronucleus of Paramecium caudatum. In this paper the release of the infectious form (IF) of H. elegans from its host is described. During the anaphase of the micronuclear division most of the IFs remain within the connecting piece which, therefore, may be...
journal_title:European journal of protistology
pub_type: 杂志文章
doi:10.1016/S0932-4739(89)80021-5
更新日期:1989-10-27 00:00:00