Abstract：
:Recent biotechnological progress has enabled the production of spider silk proteins, spidroins, in heterologous hosts. Matrices based on recombinant spidroins support stem cell growth and are well tolerated when implanted in living tissue, thus the material is highly attractive for use in regenerative medicine. However, the matrices made are far from natural silk in terms of mechanical properties and are either spontaneously assembled, which results in heterogeneous products, or spun from harsh solvents with the concomitant risk of harmful remnants in the final products. If we could mimic the spider's aqueous silk spinning process we would likely obtain a material that had reproducible and better characteristics and that more easily could be transferred to clinical practice. Herein, the knowledge of the spiders' silk production system and the prerequisites for artificial spinning and assembly of recombinant proteins are reviewed and discussed in a biomedical context.
journal_name
Acta Biomaterjournal_title
Acta biomaterialiaauthors
Rising Adoi
10.1016/j.actbio.2013.09.030subject
Has Abstractpub_date
2014-04-01 00:00:00pages
1627-31issue
4eissn
1742-7061issn
1878-7568pii
S1742-7061(13)00490-Xjournal_volume
10pub_type
杂志文章,评审abstract：:Calcium phosphate glass-based materials in the pyrophosphate region are briefly reviewed. Calcium pyrophosphate glasses can be prepared by including a small amount of TiO(2) ( journal_title：Acta biomaterialia pub_type： 杂志文章,评审 doi：10.1016/j.actbio.2004.08.001 更新日期：2005-01-01 00:00:00
abstract：UNLABELLED:Chondrocyte dedifferentiation presents a major barrier in engineering functional cartilage constructs. To mitigate the effects of dedifferentiation, this study employed a post-expansion aggregate culture step to enhance the chondrogenic phenotype of passaged articular chondrocytes (ACs) before their integrat...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.07.044
更新日期：2016-10-01 00:00:00
abstract：:Corneal disease is a major cause of blindness and keratoplasty is an effective treatment method. However, clinical treatment is limited due to a severe shortage of high-quality allogeneic corneal tissues and the bacterial infection after corneal transplantation. In this study, we develop a novel artificial and antibac...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2013.08.033
更新日期：2014-01-01 00:00:00
abstract：:The surface of an implant device can be modified by immobilizing biological molecules on it to improve its integration into the host tissue. We have previously demonstrated that enzymatically tailored plant pectins are promising nanocoatings for biomaterials. This study investigates whether a coating of modified hairy...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.03.031
更新日期：2009-09-01 00:00:00
abstract：:Adhesive resin-based restorative materials have the potential to considerably strengthen teeth and offer more economically viable alternatives to traditional materials such as gold, amalgam or ceramics. Other advantages are direct and immediate placement and the elimination of the use of mercury. However, polymerizati...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2007.06.011
更新日期：2008-01-01 00:00:00
abstract：BACKGROUND:Mechanical characteristics of vascular tissue may play a role in different arterial pathologies, which, amongst others, requires robust constitutive descriptions to capture the vessel wall's anisotropic and non-linear properties.Specifically, the complex 3D network of collagen and its interaction with other ...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.01.030
更新日期：2016-03-01 00:00:00
abstract：UNLABELLED:A new a biocompatible Ti42Zr40Ta3Si15 (atomic %) porous bulk glassy alloy was produced by combination of rapid solidification and powder metallurgy techniques. Amorphous alloy ribbons were fabricated by melt spinning, i.e. extremely fast quenching the molten alloy with 10(6)K/s from T=1973K down to room temp...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.03.020
更新日期：2016-05-01 00:00:00
abstract：:The large, densely packed artificial surface area of artificial lungs results in rapid clotting and device failure. Surface generated nitric oxide (NO) can be used to reduce platelet activation and coagulation on gas exchange fibers, while not inducing patient bleeding due to its short half-life in blood. To generate ...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2019.04.004
更新日期：2019-05-01 00:00:00
abstract：:Extracellular matrix microfibrils are critical components of connective tissues with a wide range of mechanical and cellular signalling functions. Collagen VI is a heteromeric network-forming collagen which is expressed in tissues such as skin, lung, blood vessels and articular cartilage where it anchors cells into th...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.12.023
更新日期：2017-04-01 00:00:00
abstract：:Classically, the evaluation of cellular adhesion of cells on substrates is limited to the evaluation of cell attachment after some hours. We have claimed for several years that this evaluation is incomplete concerning the evaluation of cell adhesion and more precisely of the quality of the in vitro cell/biomaterial in...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2005.06.001
更新日期：2005-09-01 00:00:00
abstract：UNLABELLED:Novel photo-reactive phospholipid polymers were synthesized for use in the preparation of nonfouling surfaces with protein conjugation capacity. Poly[2-methacryloyloxyethyl phosphorylcholine (MPC)-ran-N-methacryloyl-(l)-tyrosinemethylester (MAT)] (P(MPC/MAT)) was synthesized by conventional radical polymeriz...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.03.023
更新日期：2016-08-01 00:00:00
abstract：:A novel processing route was proposed for the fabrication of biodegradable Mg-Ca binary alloys with high Ca contents (2-3 wt.%) in sheet form with enhanced biocorrosion resistance by tailoring their microstructures. The effective refinement and dispersion of the Mg2Ca phase in the Mg-Ca alloys using extrusion followed...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2014.09.029
更新日期：2015-01-01 00:00:00
abstract：:Synthesis of tissue adhesives had been carried out in various laboratories in the past decades but the development is currently stalled. One of the key reasons, it is believed, is that researchers have not fully understood and resolved the role of the functional groups that are responsible for good adhesion to biologi...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2008.08.001
更新日期：2009-01-01 00:00:00
abstract：:Atomic force microscopy (AFM) was used to study the morphological changes of two gram-negative pathogens, Pseudomonas aeruginosa and Escherichia coli, after exposure to nitric oxide (NO). The time-dependent effects of NO released from a xerogel coating and the concentration-dependent effects rendered by a small molecu...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.01.025
更新日期：2009-06-01 00:00:00
abstract：:A new series of beta Ti-Nb-Zr (TNZ) alloys with considerable plastic deformation ability during compression test, high elastic admissible strain, and excellent cytocompatibility have been developed for removable bone tissue implant applications. TNZ alloys with nominal compositions of Ti-34Nb-25Zr, Ti-30Nb-32Zr, Ti-28...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2015.03.023
更新日期：2015-07-01 00:00:00
abstract：:Surface modification of titanium implants to improve their fixation in bone tissue is of great interest. We present a novel approach to enhance implant performance by applying important principles of bone mineralization to biomedical coatings. As an attempt to mimic the biphasic biomineralization process, both the enz...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.03.019
更新日期：2009-09-01 00:00:00
abstract：:The transfer of nucleic acids into living cells, i.e. transfection, is a major technique in current molecular biology and medicine. As nucleic acids alone are not able to penetrate the cell membrane, an efficient carrier is needed. Calcium phosphate nanoparticles can serve as carrier due to their biocompatibility, bio...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.11.051
更新日期：2019-01-15 00:00:00
abstract：:The mechanical and electrical properties of polycaprolactone fumarate-polypyrrole (PCLF-PPy) scaffolds were studied under physiological conditions to evaluate their ability to maintain the material properties necessary for application as conductive nerve conduits. PC12 cells cultured on PCLF-PPy scaffolds were stimula...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.10.013
更新日期：2011-03-01 00:00:00
abstract：UNLABELLED:Rapid vascularization of bone graft substitutes upon implantation is one of the most important challenges to overcome in order to achieve successful regeneration of large, critical-size bone defects. One strategy for stimulating vascularization during the regeneration process is to create a hypoxic microenvi...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.03.031
更新日期：2016-05-01 00:00:00
abstract：:Two enzymes, thrombin and transglutaminase, both participate in the formation of fibrin networks and contribute to the mechanical strength of biogels. A theoretical model built from the available kinetic data showed that a competition may take place between the two enzymes for their common substrate, fibrinogen. To ev...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.08.001
更新日期：2010-02-01 00:00:00
abstract：:Star-block copolymers PEI-g-PZLL with a branched polyethylenimine (PEI) core and multiple grafted poly(ε-benzyloxycarbonyl-L-lysine) (PZLL) peripheral chains were designed, synthesized, and evaluated as nanocarriers for indomethacin (IND). In an aqueous solution, PEI-g-PZLL self-assembled into spherical nanoparticles ...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.11.038
更新日期：2017-02-01 00:00:00
abstract：:Nickel-titanium shape memory alloys (NiTi-SMAs) exhibit mechanical and chemical properties which make them attractive candidate materials for various types of biomedical applications. However, the high nickel content of NiTi-SMAs may result in adverse tissue reactions, especially when they are considered for load-bear...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2011.02.022
更新日期：2011-06-01 00:00:00
abstract：:A series of six copolymeric families, P(AN-co-MTAs) with various molar fractions of acrylonitrile (fAN) and methacrylates (fMTA) based on 1,3-thiazole and 1,2,3-triazole pendant groups with several spacers of different length and nature (alkyl or succinic), have been synthesized by conventional radical polymerization....
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2015.07.037
更新日期：2015-10-01 00:00:00
abstract：:In vitro degradation and chondrocyte-encapsulation of chitosan hydrogel made of crosslinkable and water-soluble chitosan derivative (CML) at neutral pH and body temperature were studied with respect to weight loss, cytoviability, DNA content and cell morphology. In vitro degradation of the chitosan hydrogels was sensi...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2006.06.007
更新日期：2007-01-01 00:00:00
abstract：UNLABELLED:Natural amino acids are zwitterionic molecules and the good biocompatibility promises them potential candidates as anti-fouling materials. Here, we developed a new method to functionalize silica nanoparticles with a natural amino acid-based anti-fouling layer. Amino acids were covalently immobilized on 3-ami...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.03.035
更新日期：2016-08-01 00:00:00
abstract：:Using novel nitric oxide (NO)-generating polymeric hydrogels that can be rapidly photopolymerized in situ, we can deliver NO locally at the site of vascular injury. Depending on material design, these poly(ethylene glycol) (PEG)-based hydrogels can generate NO for up to 50 d. This study demonstrates the ability of nit...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2005.07.010
更新日期：2005-11-01 00:00:00
abstract：:The gradient localization of biological cues is of paramount importance to guide directional migration of cells. In this study, poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)-block- poly(2-hydroxyethyl methacrylate) (P(HEMA-co-GMA)-b-PHEMA) brushes with a uniform underneath P(HEMA-co-GMA) layer and a gradi...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.05.046
更新日期：2018-07-15 00:00:00
abstract：:Cardiac fibrosis, an important pathological feature of structural remodeling, contributes to ventricular stiffness, diastolic dysfunction, arrhythmia and may even lead to sudden death. Matrix stiffness, one of the many mechanical factors acting on cells, is increasingly appreciated as an important mediator of myocardi...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2014.01.031
更新日期：2014-06-01 00:00:00
abstract：:Owing to its bio- and osteoconductivity, hydroxyapatite (HA) is a widely used implant material, but its osteogenic properties are only partly evaluated in vitro and in vivo. The present study focused on bone healing adjacent to HA-coated titanium (Ti) implants, with or without incorporated lithium ions (Li(+)). Specia...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2013.12.012
更新日期：2014-03-01 00:00:00
abstract：:Semiconducting nanoparticles, more commonly known as quantum dots, possess unique size and shape dependent optoelectronic properties. In recent years, these unique properties have attracted much attention in the biomedical field to enable real-time tissue imaging (bioimaging), diagnostics, single molecule probes, and ...
journal_title：Acta biomaterialia
pub_type： 杂志文章,评审
doi：10.1016/j.actbio.2019.05.022
更新日期：2019-08-01 00:00:00
