Abstract：
:A liquid chromatographic method was developed for the simultaneous separation of curcumin, β-arteether, tetrahydrocurcumin and dihydroartemisinin based on the design of experiments and the design space methodology. The influence of the percentage of organic modifier, flow rate of the mobile phase and column temperature on the analytes separation was investigated. The optimal chromatographic separation was achieved on a C18 column (125mm×4mm, 5μm) using an isocratic elution with a mobile phase consisting of methanol-ammonium acetate (pH 4; 10mM) (80:20, v/v) at a flow rate of 0.45ml/min and a column temperature of 32.5°C. This method was then validated for simultaneous quantification of curcumin and β-arteether contained in lipid-based formulations taking into account the β-expectation tolerance interval for the total error measurement. Finally, the suitability of the proposed liquid chromatographic method for routine analysis of curcumin and β-arteether loaded in lipid-based formulations has been proven.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Memvanga PB,Mbinze JK,Rozet E,Hubert P,Préat V,Marini RDdoi
10.1016/j.jpba.2013.09.009subject
Has Abstractpub_date
2014-01-01 00:00:00pages
447-56eissn
0731-7085issn
1873-264Xpii
S0731-7085(13)00434-2journal_volume
88pub_type
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