Plant regeneration from long-term suspension cultures of white clover.

Abstract:

:A genotype of Trifolium repens L. capable of sustaining high-frequency plant regeneration from long-term (24-month old) cell cultures has been selected. Numerous densely cytoplasmic meristemoids were formed in suspension cultures following the coordinate removal of 2,4-dichlorophenoxyacetic acid (2,4-D) and trichloropicolinic acid (picloram) from the medium and an increase in the NH 4 (+) concentration. Some meristemoids arose from single cells in culture. Increasing the NH 4 (+) concentration in the medium resulted in increased meristemoid formation and decreased the growth rate. Ammonium stimulated meristemoid formation when it was the sole source of nitrogen only if a lethal shift in the pH of the medium was prevented. Meristemoids plated on hormone-free agar medium developed directly into shoots which spontaneously formed roots.

journal_name

Planta

journal_title

Planta

authors

White DW

doi

10.1007/BF00397413

subject

Has Abstract

pub_date

1984-09-01 00:00:00

pages

1-7

issue

1

eissn

0032-0935

issn

1432-2048

journal_volume

162

pub_type

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