Abstract:
:A genotype of Trifolium repens L. capable of sustaining high-frequency plant regeneration from long-term (24-month old) cell cultures has been selected. Numerous densely cytoplasmic meristemoids were formed in suspension cultures following the coordinate removal of 2,4-dichlorophenoxyacetic acid (2,4-D) and trichloropicolinic acid (picloram) from the medium and an increase in the NH 4 (+) concentration. Some meristemoids arose from single cells in culture. Increasing the NH 4 (+) concentration in the medium resulted in increased meristemoid formation and decreased the growth rate. Ammonium stimulated meristemoid formation when it was the sole source of nitrogen only if a lethal shift in the pH of the medium was prevented. Meristemoids plated on hormone-free agar medium developed directly into shoots which spontaneously formed roots.
journal_name
Plantajournal_title
Plantaauthors
White DWdoi
10.1007/BF00397413subject
Has Abstractpub_date
1984-09-01 00:00:00pages
1-7issue
1eissn
0032-0935issn
1432-2048journal_volume
162pub_type
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