Methylglyoxal concentrations differ in standard and washed neonatal packed red blood cells.

Abstract:

BACKGROUND:Preterm infants have a greater risk of necrotizing enterocolitis following transfusion. It is hypothesized that high glucose concentrations in red blood cell (RBC) preservatives lead to increased methylglyoxal (MG) metabolism, causing glycation-driven damage to transfused RBCs. Such changes to the RBCs could promote a proinflammatory state in transfusion recipients. METHODS:Standard and washed RBCs in Adsol-3, two common neonatal preparations, were studied. Consecutive measurements were performed of glucose, MG, reduced glutathione, glyoxalase I activity (GLO-I), and D-lactate, the stable end product of MG detoxification by glyoxalase enzymes over the 42-d storage period. RESULTS:RBC units consume glucose and produceD-lactate and MG during storage. In 28/30 units, the MG concentrations showed only small variations during storage. Two units had elevated MG levels (>10 pmol/mg Hb) during the first half of storage. Washing of the RBCs significantly reduced both MG and D-lactate. CONCLUSION:This study shows two patterns of MG metabolism in packed RBCs for neonatal transfusion and raises the possibility that RBC units with higher MG levels may have increased glycation-driven damage in the transfused RBCs. Whether transfused MG could trigger an inflammatory response such as necrotizing enterocolitis in preterm neonates and whether washing could prevent this require further study.

journal_name

Pediatr Res

journal_title

Pediatric research

authors

Kiefer AS,Fleming T,Eckert GJ,Poindexter BB,Nawroth PP,Yoder MC

doi

10.1038/pr.2013.243

subject

Has Abstract

pub_date

2014-03-01 00:00:00

pages

409-14

issue

3

eissn

0031-3998

issn

1530-0447

pii

pr2013243

journal_volume

75

pub_type

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