Abstract:
BACKGROUND:Preterm infants have a greater risk of necrotizing enterocolitis following transfusion. It is hypothesized that high glucose concentrations in red blood cell (RBC) preservatives lead to increased methylglyoxal (MG) metabolism, causing glycation-driven damage to transfused RBCs. Such changes to the RBCs could promote a proinflammatory state in transfusion recipients. METHODS:Standard and washed RBCs in Adsol-3, two common neonatal preparations, were studied. Consecutive measurements were performed of glucose, MG, reduced glutathione, glyoxalase I activity (GLO-I), and D-lactate, the stable end product of MG detoxification by glyoxalase enzymes over the 42-d storage period. RESULTS:RBC units consume glucose and produceD-lactate and MG during storage. In 28/30 units, the MG concentrations showed only small variations during storage. Two units had elevated MG levels (>10 pmol/mg Hb) during the first half of storage. Washing of the RBCs significantly reduced both MG and D-lactate. CONCLUSION:This study shows two patterns of MG metabolism in packed RBCs for neonatal transfusion and raises the possibility that RBC units with higher MG levels may have increased glycation-driven damage in the transfused RBCs. Whether transfused MG could trigger an inflammatory response such as necrotizing enterocolitis in preterm neonates and whether washing could prevent this require further study.
journal_name
Pediatr Resjournal_title
Pediatric researchauthors
Kiefer AS,Fleming T,Eckert GJ,Poindexter BB,Nawroth PP,Yoder MCdoi
10.1038/pr.2013.243subject
Has Abstractpub_date
2014-03-01 00:00:00pages
409-14issue
3eissn
0031-3998issn
1530-0447pii
pr2013243journal_volume
75pub_type
杂志文章abstract:BACKGROUND:Scoring tools used to quantify withdrawal in infants with neonatal abstinence syndrome (NAS) are often confounded by subjective measurements. This study assessed salivary cortisol as an objective biomarker of withdrawal severity in opioid-exposed newborns. METHODS:A prospective study was conducted in 25 ful...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/s41390-019-0601-7
更新日期:2020-05-01 00:00:00
abstract::This study was designed to evaluate upper airway protective mechanisms in response to pharyngeal fluid stimulation in healthy term and preterm infants at term equivalent age. Five term and seven preterm infants were studied and the following recorded: sleep state, cardiorespiratory function, and swallowing. Infusions ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199811000-00011
更新日期:1998-11-01 00:00:00
abstract::Seven essentially healthy term infants who received gentamicin starting on the 1st day of life for prolonged rupture of membranes and maternal fever were compared with nine healthy term infants to determine whether this drug induces alterations in the auditory pathway. The auditory pathway was studied on the 3rd day o...
journal_title:Pediatric research
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abstract::Milk gangliosides inhibit Vibrio cholerae enterotoxin and Escherichia coli heat-labile enterotoxin. Human milk gangliosides showed considerably higher enterotoxin-inhibitory activity compared to bovine and formula milk gangliosides as measured in vitro by enzyme-linked immunosorbent assay and in vivo in rabbit small b...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198605000-00008
更新日期:1986-05-01 00:00:00
abstract::Conversion of adenosine to inosine is decreased in adenosine deaminase (ADA)-deficient fibroblasts at all concentrations of adenosine tested. Adenosine is not differentially toxic to ADA-deficient fibroblasts except at very high (5 X 10(-4) -1 X 10(-3) M) adenosine levels. Conversion of [14C] adenosine to GTP is not d...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-197607000-00002
更新日期:1976-07-01 00:00:00
abstract::Fifty patients with haemophilia B, belonging to 29 kindreds, were investigated with a highly sensitive immunoradiometric assay based on a homologous antibody to factor IX. The assay measures factor IX antigen (f.IX:Ag) in plasma down to 0.025 U/dl. Seventeen of 18 investigated patients with severe haemophilia B had ve...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198203000-00019
更新日期:1982-03-01 00:00:00
abstract::Epidermal growth factor (EGF), a potent mitogen present in milk, is postulated to play an important physiologic role in the ontogeny of the intestine. An artificial rat milk, free of EGF, was developed and fed to newborn rats in an effort to evaluate the physiologic role of EGF on intestinal glucose transport. Compari...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198704000-00017
更新日期:1987-04-01 00:00:00
abstract:BACKGROUND:Deficiencies in phagocytosis may contribute to the increased susceptibility of infants to early life infections. Data on phagocytosis of the major neonatal pathogens Staphylococcus epidermidis (SE), Staphylococcus aureus (SA), and Escherichia coli (EC) by preterm infant leukocytes are inconsistent. METHODS:...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2013.145
更新日期:2013-11-01 00:00:00
abstract::The relationship between cerebral blood flow (CBF) and the evolution of brain edema was investigated in an experimental model of perinatal hypoxic-ischemic brain damage. Seven-d postnatal rats were subjected to unilateral common carotid artery ligation followed by 3 h of hypoxia with 8% oxygen at 37 degrees C. This in...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199005000-00007
更新日期:1990-05-01 00:00:00
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journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199811000-00021
更新日期:1998-11-01 00:00:00
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journal_title:Pediatric research
pub_type: 临床试验,杂志文章
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更新日期:2007-11-01 00:00:00
abstract:INTRODUCTION:Very early preterm infants (VPIs) are exposed to unpredictable noise in neonatal intensive care units. Their ability to perceive moderate acoustic environmental changes has not been fully investigated. RESULTS:Physiological values of the 598 isolated sound peaks (SPs) that were 5-10 and 10-15 dB slow-resp...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2011.76
更新日期:2012-04-01 00:00:00
abstract::The lymphocytes of human breast milk have been previously shown to be immunologically competent while the monocytes have been described as actively motile in some reports but not in another. Cells were separated from milk samples collected by lactating women 2 to 9 days postpartum. Milk cell migration and production o...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198510000-00006
更新日期:1985-10-01 00:00:00
abstract::Platelet volume was measured in 20 healthy children, 18 patients with acute idiopathic thrombocytopenic purpura (ITP), 24 patients with chronic ITP, 22 patients with aplastic anemia, and 17 patients with acute leukemia in childhood. The authors compared the platelet volume of these patients by use of peak platelet vol...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198002000-00013
更新日期:1980-02-01 00:00:00
abstract::Mucoid Pseudomonas aeruginosa is an important respiratory pathogen in patients with cystic fibrosis, and once acquired is virtually impossible to eradicate. Although mucoid P. aeruginosa is generally believed to be resistant to phagocytosis, the mechanism is not understood fully. We studied the nonopsonic phagocytosis...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198710000-00013
更新日期:1987-10-01 00:00:00
abstract::This study was aimed at describing abdominal and laryngeal muscle responses to upper airway occlusion (UAO) in early life and the effect of sleep states on these responses. Twelve nonsedated, 9-26-d-old lambs were studied. We simultaneously recorded 1) airflow (pneumotachograph + face mask); 2) sleep states (electroco...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199706000-00011
更新日期:1997-06-01 00:00:00
abstract::In order to study the neonatal microcirculation, the capillary hemodynamics in skin was investigated in 43 full-term infants 2-7 days after birth. The nailfold capillaries of the thumb were visualized by means of television microscopy and the capillary blood cell velocity (CBV) was videophotometrically quantified in 1...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198806000-00011
更新日期:1988-06-01 00:00:00
abstract:BACKGROUND:To investigate relationships between infant body composition (BC) and human milk (HM) immunomodulatory proteins (IMPs) during the first 12 months of lactation. METHODS:BC of breastfeeding dyads (n = 20) was measured with ultrasound skinfolds (infants) and bioimpedance spectroscopy (infants/mothers) at 2, 5,...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/s41390-020-0961-z
更新日期:2020-05-21 00:00:00
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journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199403000-00010
更新日期:1994-03-01 00:00:00
abstract::The perinatal ontogeny of preprotachykinin-A gene expression was assessed in central respiration-related structures. On the day of birth, there was an enhanced expression of preprotachykinin-A mRNA in the region of the nucleus tractus solitarii, the primary relay station for respiratory and cardiovascular reflexes. Th...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199104000-00008
更新日期:1991-04-01 00:00:00
abstract::This report deals with quantitative and qualitative investigations of alkaline phosphatase in two unrelated infants with the severe infantile form of hypophosphatasia. Both affected infants had no detectable leukocyte alkaline phosphatase activities and both sets of parents and one sibling tended to have low but varia...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-197607000-00004
更新日期:1976-07-01 00:00:00
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journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198904000-00012
更新日期:1989-04-01 00:00:00
abstract::Serum insulin, growth hormone (GH), insulin-like growth factors (IGFs) I and II, cortisol, and albumin concentrations were measured in 15 children with kwashiorkor, 15 with marasmic-kwashiorkor, and 21 with marasmus, before and in the survivors, after nutritional rehabilitation, as well as in 10 underweight and eight ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198611000-00012
更新日期:1986-11-01 00:00:00
abstract::The effect of Cis-platin on the glomerular filtration rate and effective renal plasma flow was determined using a radioisotope clearance technique in young (3 wk old) and adult (more than 12 wk old) rats. Cis-platin was administered intravenously in dosages ranging from 2.5 to 10 mg/kg body weight, either as a single ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198601000-00003
更新日期:1986-01-01 00:00:00
abstract:BACKGROUND:There is a strong need for continuous cerebral circulation monitoring in neonatal care, since suboptimal cerebral blood flow may lead to brain injuries in preterm infants and other critically ill neonates. NeoDoppler is a novel ultrasound system, which can be gently fixed to the anterior fontanel and measure...
journal_title:Pediatric research
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doi:10.1038/s41390-019-0535-0
更新日期:2020-01-01 00:00:00
abstract::A rabbit model of group B Streptococcal (GBS) shock was used to determine if myocardial dysfunction contributes to GBS shock and, if so, to ascertain if prostaglandins modulate this dysfunction. The infusion of heat-killed GBS (group I) produced a dramatic decrease in the first derivative of left ventricular pressure ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198506000-00001
更新日期:1985-06-01 00:00:00
abstract::Labeled choline incorporation into adult rat lung alveolar epithelial cells and adult rat lung fibroblasts in monolayer culture was determined after incubation with insulin (Ins) 10 micrograms/ml, Dexamethasone (Dex) 10(-6)M, or no drug (ND). Incubation periods were 1, 3, 4, and 5 hours. The lecithin (phosphatidyl cho...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198007000-00014
更新日期:1980-07-01 00:00:00
abstract::Injuries continue to be the leading cause of morbidity and mortality for children, adolescents, and young adults aged 1-24 years in industrialized countries in the twenty-first century. In this age group, injuries cause more fatalities than all other causes combined in the United States (U.S.). Importantly, many of th...
journal_title:Pediatric research
pub_type: 杂志文章,评审
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更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:The aim of this study is to compare new pediatric nomograms for clinical parameters from 2D echocardiography. METHODS:2D pediatric echocardiographic parameters from four recent nomograms were used for statistical analysis. To assess the accuracy of the predictive models from each study, namely multivariate,...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/s41390-020-0900-z
更新日期:2020-04-24 00:00:00
abstract:BACKGROUND:Extremely preterm birth is associated with subsequent behavioral problems. We hypothesized that perinatal systemic inflammation, a risk factor for cerebral white matter injury and cognitive impairment, is associated with behavior problems observed at 2 y. METHODS:In a cohort of 600 children born before 28 w...
journal_title:Pediatric research
pub_type: 杂志文章,多中心研究
doi:10.1038/pr.2014.41
更新日期:2014-06-01 00:00:00