Abstract:
:Vitamin B9, commonly known as folate, is an essential cofactor for one-carbon metabolism that enters cells through three major specialized transporter molecules (RFC, FR, and PCFT), which differ in expression pattern, affinity for substrate, and ligand-binding pH dependency. We now report that the expression of the folate transporters differs between macrophage subtypes and explains the higher accumulation of 5-MTHF-the major folate form found in serum-in M2 macrophages in vitro and in vivo. M1 macrophages display a higher expression of RFC, whereas FRβ and PCFT are preferentially expressed by anti-inflammatory and homeostatic M2 macrophages. These differences are also seen in macrophages from normal tissues involved in folate transit (placenta, liver, colon) and inflamed tissues (ulcerative colitis, RA), as M2-like macrophages from normal tissues express FRβ and PCFT, whereas TNF-α-expressing M1 macrophages from inflamed tissues are RFC+. Besides, we provide evidences that activin A is a critical factor controlling the set of folate transporters in macrophages, as it down-regulates FRβ, up-regulates RFC expression, and modulates 5-MTHF uptake. All of these experiments support the notion that folate handling is dependent on the stage of macrophage polarization.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Samaniego R,Palacios BS,Domiguez-Soto Á,Vidal C,Salas A,Matsuyama T,Sánchez-Torres C,de la Torre I,Miranda-Carús ME,Sánchez-Mateos P,Puig-Kröger Adoi
10.1189/jlb.0613345subject
Has Abstractpub_date
2014-05-01 00:00:00pages
797-808issue
5eissn
0741-5400issn
1938-3673pii
jlb.0613345journal_volume
95pub_type
杂志文章abstract::The aim of this study was to test the hypothesis whether MERTK, which is up-regulated in human DCs treated with immunosuppressive agents, is directly involved in modulating T cell activation. MERTK is a member of the TAM family and contributes to regulating innate immune response to ACs by inhibiting DC activation in ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.3A0714-334R
更新日期:2015-04-01 00:00:00
abstract::The kinetics and regulatory mechanisms of T cell migration through the endothelium have not been fully defined. In experimental, filter-based assays in vitro, transmigration of lymphocytes takes hours, compared with minutes, in vivo. We cultured endothelial cell (EC) monolayers on filters, solid substrates, or collage...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0508301
更新日期:2009-01-01 00:00:00
abstract::Interaction of bacterial lipopolysaccharide (LPS) with monocytes stimulates production of a variety of mediators that are involved in the pathogenesis of septic shock and wound repair. We report here the mechanisms of LPS uptake and intracellular distribution of LPS in human monocytes. Ficoll-Hypaque-purified peripher...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.48.4.316
更新日期:1990-10-01 00:00:00
abstract::In this report, the construction of a functional, immunocompetent, full-thickness skin equivalent (SE) is described, consisting of an epidermal compartment containing keratinocytes, melanocytes, and human LCs derived from the MUTZ-3 cell line (MUTZ-LC) and a fibroblast-populated dermal compartment. The CD1a(+)Langerin...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0610374
更新日期:2011-11-01 00:00:00
abstract::Recent studies have shown that proinflammatory cytokines damage rodent neural precursor cells (NPCs), a source of self-renewing, multipotent cells that play an important role in the developing as well as adult brain. In this study, the effects of tumor necrosis factor alpha (TNF-alpha) on cytokine and chemokine produc...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0405221
更新日期:2005-12-01 00:00:00
abstract::The mechanisms by which concanavalin A (Con A), a lectin, activates T cells are poorly studied. A low dose of Con A is stimulatory for T cells, whereas a high dose of Con A results in suppression of proliferation and enhanced T cell death. The expression and functional roles of costimulatory receptors, CD28 and cytoto...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.1104644
更新日期:2005-07-01 00:00:00
abstract::IL-18 has a potential to up-regulate the Th1 and Th2 immune responses. It is known that IL-18, in synergy with IL-12, augments the Th1 response to bacterial infections, but it also augments the Th2 response to allergic disorders in the absence of IL-12. Although the Th1 and Th2 immune responses cross-regulate each oth...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1189/jlb.1012502
更新日期:2013-05-01 00:00:00
abstract::Tumor necrosis factor (TNF)-stimulated gene 14 (TSG-14, also termed PTX3) encodes a secreted glycoprotein whose carboxy-terminal half shares sequence similarity with the pentraxin family of acute phase proteins (C-reactive protein and serum amyloid P component). We compared TSG-14 mRNA expression in cultures of murine...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.67.3.387
更新日期:2000-03-01 00:00:00
abstract::Individuals with T2D and PD suffer significantly from the ability of one disease to intensify the other. Disease-associated inflammation is one mechanism thought to fuel this pathogenic feed-forward loop. Several lines of evidence indicate that proinflammatory B cells promote T2D and PD; thus, B cells are top candidat...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.4A0214-095R
更新日期:2014-08-01 00:00:00
abstract::This study investigated the expression of MUC5B by AMs in the lungs of cigarette smokers and nonsmokers. We analyzed MUC5B expression by measuring the levels of apomucin and mRNA in human BALF cells from 50 subjects (20 nonsmokers, 17 patients with CB, and 13 patients with COPD). apoMUC5B was observed in BALF mononucl...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0111047
更新日期:2012-08-01 00:00:00
abstract::Galectin-3 (gal-3), a beta-galactoside-binding animal lectin, plays a role in cell-cell and cell-extracellular matrix interactions. Extracellular gal-3 modulates cell migration and adhesion in several physiological and pathological processes. Gal-3 is highly expressed in activated macrophages. Schistosoma mansoni eggs...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.1206747
更新日期:2007-08-01 00:00:00
abstract::Brucella abortus is a Gram-negative intracellular bacterial pathogen that causes a zoonosis of worldwide occurrence, leading to undulant fever in humans and abortion in domestic animals. B. abortus is recognized by several pattern-recognition receptors triggering pathways during the host innate immune response. Theref...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.4A0815-346R
更新日期:2016-05-01 00:00:00
abstract::Although mouse models of inflammatory skin diseases such as psoriasis and atopic dermatitis fail to completely phenocopy disease in humans, they provide invaluable tools to examine the molecular and cellular mechanisms responsible for the epidermal hyperplasia, inflammation, and excess angiogenesis observed in human d...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0607347
更新日期:2008-07-01 00:00:00
abstract::We provide new information on how apoptosis regulates the expansion and survival of dendritic cell (DC) elements during in vitro hematopoiesis. Functionally distinct apoptotic schedules were associated with different phases of DC development when multipotent CD34+ progenitor cells were treated with GM-CSF + TNF +/- SC...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.62.4.493
更新日期:1997-10-01 00:00:00
abstract::Vertebrate lectin purified from loach egg was tested for induction of tumor lysis mediated by macrophages. Loach egg lectin lysed tumor cells but not normal spleen cells in cooperation with BCG- or glucan (TAK)-elicited peritoneal macrophages of mice. Corynebacterium parvum-, OK432-, glycogen-, lipopolysaccharide-elic...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.35.3.241
更新日期:1984-03-01 00:00:00
abstract::Alzheimer's disease (AD) is a progressive, neurodegenerative disease characterized by the presence of multiple senile plaques in the brain tissue, which are also associated with considerable inflammatory infiltrates. Although the precise mechanisms of the pathogenesis of AD remain to be determined, the overproduction ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:
更新日期:2002-10-01 00:00:00
abstract::Constitutive expression of cell-associated, but not secreted, interleukin-1alpha (IL-1alpha) by oncogene-transformed fibrosarcoma cells induced regressing tumors in mice, a phenomenon that was abrogated by the IL-1 inhibitor, the IL-1 receptor antagonist (IL-1Ra). On the contrary, non-IL-1alpha-expressing tumor cells ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0905509
更新日期:2006-07-01 00:00:00
abstract::The relationship between leukocyte migration and parenchymal cell death in vivo remains poorly documented. Accordingly, cell killing in the rat mesentery, as recorded by propidium iodide staining, was investigated with an intravital approach. Superfusion of platelet-activating factor (PAF, 10(-8) M) or N-formyl-methio...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.62.2.163
更新日期:1997-08-01 00:00:00
abstract::Respiratory complications occur frequently in individuals living with human immunodeficiency-1 virus (HIV) infection, and there is evidence that HIV-related oxidative stress impairs alveolar macrophage immune function. We hypothesized that nuclear factor (erythroid-derived 2)-like 2 (Nrf2), a master transcription fact...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.4A0616-282RR
更新日期:2017-08-01 00:00:00
abstract::Dynamic rearrangements of the actin cytoskeleton are crucial for the function of numerous cellular elements including T lymphocytes. They are required for migration of T lymphocytes through the body to scan for the presence of antigens, as well as for the formation and stabilization of the immunological synapse at the...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1189/jlb.0602272
更新日期:2003-01-01 00:00:00
abstract::The neuropathogenesis of HIV-1 encephalitis and its associated dementia revolves around sustained viral replication in cells of mononuclear phagocyte origin (brain macrophages, multinucleated giant cells, and microglia). Macrophage secretory factors play important roles in facilitating monocyte trafficking into the br...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.62.1.100
更新日期:1997-07-01 00:00:00
abstract::Fluid-phase endocytosis is stimulated by H-ras-linked growth factor receptors and this stimulation requires activation of rab5. We utilized a GFP-rab5a:wt fusion protein to monitor GFP-rab5a:wt activation in living fibroblasts and in J774 macrophages. Control CHO cells that expressed GFP-rab5a:wt were cultured in seru...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract::High mobility group box 1 (HMGB1) is one of the recently defined damage-associated molecular pattern molecules, passively released from necrotic cells and secreted by activated macrophage/monocytes. Whether cytolytic cells induce HMGB1 release from tumor cells is not known. We developed a highly sensitive method for d...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0306169
更新日期:2007-01-01 00:00:00
abstract::NK cells are members of the innate immunity and play a central role in the defense against viral infections and cancer development, but also contribute to triggering and shaping adaptive immune responses. Human NK cells may express MHC II and costimulatory molecules, including CD86, CD80, and OX40 ligand, which allows...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/JLB.4A0417-163RR
更新日期:2018-01-01 00:00:00
abstract::Mycoplasmas and mycoplasma membranes have been shown to induce the production of inflammatory cytokines, including tumor necrosis factor alpha and interleukin-6, as well as nitric oxide, by mouse macrophages and rat brain astrocytes. Luminol-enhanced chemiluminescence was used as a sensitive method to show that Mycopl...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.57.2.264
更新日期:1995-02-01 00:00:00
abstract::Workshop cluster 1 (WC1) is a member of the scavenger receptor cysteine-rich (SRCR) superfamily that includes CD5, CD6, CD163, and M160. Bovine WC1 consists of 11 SRCR domains, a unique domain 1, and two homologous 5 SRCR domain cassettes, WC1 domains 2-6 and 7-11. The porcine orthologue of WC1 contains five SRCR doma...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
更新日期:2002-08-01 00:00:00
abstract::Bone destruction in inflammatory osteolytic diseases including periodontitis is related to excessive activity of osteoclasts (OC), which originate from precursor cells of the myeloid lineage, termed osteoclast precursors (OCP). In contrast to ample knowledge that we currently have on mature OC, little is known about O...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/JLB.1HI0620-230R
更新日期:2020-10-01 00:00:00
abstract::Monocyte chemoattractant protein-1 (MCP-1, CCL2) is a mediator of inflammation that has been implicated in the pathogenesis of a wide variety of human diseases. CCR2, a heterotrimeric G-coupled receptor, is the only known receptor that functions at physiologic concentrations of MCP-1. Despite the importance of CCR2 in...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0903421
更新日期:2004-06-01 00:00:00
abstract::In an analysis of nitric oxide (.NO) production and toxicity, chicken macrophage-generated .NO inhibited mitochondrial activity in both .NO-producing macrophages themselves and lymphoid tumor targets. However, differences in targeting of mitochondrial toxicity were observed among these cells. Two chicken macrophage ce...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.56.1.52
更新日期:1994-07-01 00:00:00
abstract::The oncogene EVI1 has been implicated in the etiology of AML and MDS. Although AML cells are characterized by accelerated proliferation and differentiation arrest, MDS cells hyperproliferate when immature but fail to differentiate later and die instead. In agreement with its roles in AML and in immature MDS cells, EVI...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0109042
更新日期:2009-10-01 00:00:00