Abstract:
:White adipose tissue (WAT) is a heterogeneous tissue composed of lipid-filled adipocytes and several nonadipocyte cell populations, including endothelial, blood, uncharacterized stromal, and adipocyte precursor cells. Although lipid-filled adipocytes account for the majority of WAT volume and mass, nonadipocyte cell populations have critical roles in WAT maintenance, growth, and function. As mature adipocytes are terminally differentiated postmitotic cells, differentiation of adipocyte precursors is required for hyperplastic WAT growth during development and in obesity. In this chapter, we present methods to separate adipocyte precursor cells from other nonadipocyte cell populations within WAT for analysis by flow cytometry or purification by fluorescence-activated cell sorting. Additionally, we provide methods to study the adipogenic capacity of purified adipocyte precursor cells ex vivo.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Church CD,Berry R,Rodeheffer MSdoi
10.1016/B978-0-12-411619-1.00003-3subject
Has Abstractpub_date
2014-01-01 00:00:00pages
31-46eissn
0076-6879issn
1557-7988pii
B978-0-12-411619-1.00003-3journal_volume
537pub_type
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