Abstract:
:Mitogen‑activated protein kinase‑activated protein kinase 2 (MK2) and its mediated inflammation are involved in various diseases, including spinal cord injury (SCI). Ultrashortwave (USW) radiation has previously been reported to exert a protective effect on SCI. In the present study, through a series of reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), western blot and immunofluorescence assay, it was found that MK2 and tumor necrosis factor (TNF)‑α/interleukin (IL)‑1β were elevated in patients with SCI and in H2O2‑treated C8‑D1A cells. Through gene level and protein level detection by using of RT‑qPCR, western blot, immunofluorescence assay and terminal deoxynucleotidyl transferase (TdT) dUTP nick‑end labeling assay, it was demonstrated that USW radiation inhibited the expression of MK2/TNF‑α/IL‑1β and suppressed the apoptosis of H2O2‑treated C8‑D1A cells. Furthermore, it was confirmed that the overexpression of MK2 reversed the protective effect of USW on C8‑D1A cells, which indicated that USW achieved its function via regulation of the MK2/TNF‑α/IL‑1β pathway. Finally, using a constructed in vivo model and a series of RT‑qPCR, western blot and IHC detection, it was confirmed that USW suppressed the expression of MK2 to promote functional recovery following SCI.
journal_name
Int J Mol Medjournal_title
International journal of molecular medicineauthors
Wang N,Feng Z,Zhao W,Zhang Z,Zhang Ldoi
10.3892/ijmm.2018.3786subject
Has Abstractpub_date
2018-10-01 00:00:00pages
1909-1916issue
4eissn
1107-3756issn
1791-244Xjournal_volume
42pub_type
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