Abstract：
:Filamentous fungi are used in several industries and in academia to produce antibiotics, metabolites, proteins and pharmaceutical compounds. The development of valuable strains usually requires the insertion of recombinant deoxyribonucleic acid; however, the protocols to transfer DNA to fungal cells are highly inefficient. Recently, underwater shock waves were successfully used to genetically transform filamentous fungi. The purpose of this research was to demonstrate that the efficiency of transformation can be improved significantly by enhancing acoustic cavitation using tandem (dual-pulse) shock waves. Results revealed that tandem pressure pulses, generated at a delay of 300 μs, increased the transformation efficiency of Aspergillus niger up to 84% in comparison with conventional (single-pulse) shock waves. This methodology may also be useful to obtain new strains required in basic research and biotechnology.
journal_name
Ultrasonicsjournal_title
Ultrasonicsauthors
Loske AM,Fernández F,Magaña-Ortíz D,Coconi-Linares N,Ortíz-Vázquez E,Gómez-Lim MAdoi
10.1016/j.ultras.2014.03.003subject
Has Abstractpub_date
2014-08-01 00:00:00pages
1656-62issue
6eissn
0041-624Xissn
1874-9968pii
S0041-624X(14)00058-4journal_volume
54pub_type
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