Abstract：
:Human herpesvirus 8 (HHV8) infection leads to potent activation of nuclear factor kappa B (NFκB) in primary and transformed cells. We used recombinant HHV8 (rKSHV.219) expressing green fluorescent protein under the constitutive cellular promoter elongation factor 2α and red fluorescent protein under an early HHV8 lytic gene promoter T1.1 to monitor replication during infection of human foreskin fibroblasts (HF), noting changes in NFκB activity. In primary HF, NFκB levels do not affect the ability of HHV8 to establish infection or maintain latency. Furthermore, there was no effect on the percent of cells undergoing reactivation from latency, and there were similar numbers of released and cell-associated HHV8 viral particles following reactivation in the presence of inhibitors. Reactivation of HHV8 in latently infected HF in the presence of NFκB inhibitors resulted in production of viral particles that did not efficiently establish infection, due to deficiencies in binding and/or entry into normally permissive cells. Exogenous expression of glycoprotein M, an envelope protein involved in viral binding and entry, was able to partially overcome the deficiency induced by NFκB inhibitors. Our data indicate that in primary cells, NFκB is not required for infection, establishment of latency, or entry into the lytic cycle, but is required for the expression of virion associated genes involved in the initial steps of virion infectivity. These studies suggest that strategies to inhibit NFκB may prevent HHV8 spread and should be considered as a potential therapeutic target for preventing HHV8 associated diseases.
journal_name
Front Microbioljournal_title
Frontiers in microbiologyauthors
Blattman NN,Lagunoff M,Blattman JN,Corey Ldoi
10.3389/fmicb.2014.00129subject
Has Abstractpub_date
2014-04-04 00:00:00pages
129issn
1664-302Xjournal_volume
5pub_type
杂志文章abstract：:Neisseria gonorrhoeae infections are a serious global health problem. This organism has developed disturbing levels of antibiotic resistance, resulting in the need for new approaches to prevent and treat gonorrhea. The genus Neisseria also includes several members of the human microbiome that live in close association...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2020.577762
更新日期：2020-12-03 00:00:00
abstract：:The gastrointestinal microbiome is crucial in human health. With greater than 10 times the cell count of an individual, the gastrointestinal microbiome provides many benefits to the host. It plays an important role in chronic illnesses and immune diseases and also following burns and trauma. This study aimed to determ...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2017.01237
更新日期：2017-07-06 00:00:00
abstract：:Functional oligosaccharides, known as prebiotics, and ordinary dietary fiber have important roles in modulating the structure of intestinal microbiota. To investigate their effects on the intestinal microecosystem, three kinds of diets containing different prebiotics were used to feed mice for 3 weeks, as follows: GI ...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2017.01750
更新日期：2017-09-20 00:00:00
abstract：:This study was conducted to compare the microbiome and metabolome differences in the colon lumen from two pig breeds with different genetic backgrounds. Fourteen weaned piglets at 30 days of age, including seven Landrace piglets (a lean-type pig breed with a fast growth rate) and seven Meihua piglets (a fatty-type Chi...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2017.01812
更新日期：2017-09-21 00:00:00
abstract：:Tuberculosis (TB) recurrence can result from either relapse of an original infection or exogenous reinfection with a new strain of Mycobacterium tuberculosis (MTB). The aim of this study was to assess the roles of relapse and reinfection among recurrent TB cases characterized by a high prevalence rate of drug-resistan...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2018.01858
更新日期：2018-08-14 00:00:00
abstract：:Dietary inclusions of baicalin and chlorogenic acid were beneficial for intestinal health in pigs. Nevertheless, it is unknown whether these plant-derived products had protection for intestine against bacterial challenge in chickens. This study was aimed at evaluating the potential mitigating effects of plant extracts...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2019.01681
更新日期：2019-07-24 00:00:00
abstract：:Enterovirus A71 (EV-A71) is one of the major etiologic agents causing hand, foot, and mouth disease (HFMD) in children and occasionally causes severe neurological diseases or even death. EV-A71 replicates rapidly in host cells. For a successful infection, viruses produce large quantities of viral proteins in a short p...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2020.01656
更新日期：2020-07-14 00:00:00
abstract：:Salmonella Enteritidis (SE) is a facultative intracellular pathogen that colonizes the chicken gut leading to contamination of carcasses during processing. A reduction in intestinal colonization by SE could result in reduced carcass contamination thereby reducing the risk of illnesses in humans. Short chain fatty acid...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2020.553670
更新日期：2020-09-16 00:00:00
abstract：:[This corrects the article DOI: 10.3389/fmicb.2018.00516.]. ...
journal_title：Frontiers in microbiology
pub_type： 已发布勘误
doi：10.3389/fmicb.2018.03245
更新日期：2019-01-14 00:00:00
abstract：:Accurately identifying bacteriophage virion proteins from uncharacterized sequences is important to understand interactions between the phage and its host bacteria in order to develop new antibacterial drugs. However, identification of such proteins using experimental techniques is expensive and often time consuming; ...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2018.00476
更新日期：2018-03-16 00:00:00
abstract：:We report the isolation, characterization, and development of a robust genetic system for a halophilic, Fe(II)-oxidizing bacterium isolated from a vertical borehole originating 714 m below the surface located in the Soudan Iron Mine in northern Minnesota, USA. Sequence analysis of the 16S rRNA gene places the isolate ...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2015.00719
更新日期：2015-07-16 00:00:00
abstract：:The rumen and the hindgut represent two different fermentation organs in herbivorous mammals, with the former producing much more methane than the latter. The objective of this study was to elucidate the microbial underpinning of such differential methane outputs between these two digestive organs. Methane production ...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2018.00575
更新日期：2018-03-27 00:00:00
abstract：:Molasses are sub-products of the sugar industry, rich in sucrose and containing other sugars like raffinose, glucose, and fructose. Alpha-galactosidases (EC. 3.2.1.22) catalyze the hydrolysis of alpha-(1,6) bonds of galactose residues in galacto-oligosaccharides (melibiose, raffinose, and stachyose) and complex galact...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2019.00405
更新日期：2019-03-07 00:00:00
abstract：:Advanced treatment facilities for potable water reuse of wastewater are designed to achieve high removal levels of specific pathogens, as well as many other constituents. However, changes to the microbial community throughout treatment, storage, and distribution of this water have not been well characterized. We appli...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2019.00993
更新日期：2019-05-08 00:00:00
abstract：:The objective of this study was to investigate the ability of a culture highly enriched with the polyphosphate-accumulating organism, "Candidatus Accumulibacter phosphatis" clade IIC, to adjust their metabolism to different phosphate availabilities. For this purpose the biomass was cultivated in a sequencing batch rea...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2016.02121
更新日期：2017-01-05 00:00:00
abstract：:Objective: The aim of this study was to investigate the molecular mechanism of inflammasome activation in response to Streptococcus suis serotype 2 (SS2) infection and its contribution to the development of streptococcal toxic shock-like syndrome (STSS). Methods: To verify the role of suilysin (SLY) in STSS, we infect...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2020.01788
更新日期：2020-08-14 00:00:00
abstract：:Cyanobacteria are major contributors to global biogeochemical cycles. The genetic diversity among Cyanobacteria enables them to thrive across many habitats, although only a few studies have analyzed the association of phylogenomic clades to specific environmental niches. In this study, we adopted an ecogenomics strate...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2017.02132
更新日期：2017-11-14 00:00:00
abstract：:Multi-drug resistant Staphylococcus aureus (MDRSA) remains a great challenge despite a decade of research on antimicrobial compounds against their infections. In the present study, various acyclic amines and diamines were chemically synthesized and tested for their antimicrobial as well as antibiofilm activity against...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2017.01767
更新日期：2017-09-15 00:00:00
abstract：:[This corrects the article DOI: 10.3389/fmicb.2019.02612.]. ...
journal_title：Frontiers in microbiology
pub_type： 已发布勘误
doi：10.3389/fmicb.2020.00031
更新日期：2020-01-29 00:00:00
abstract：:To gain better understanding of the distributions of the culturable Lactobacillus species in the chicken intestinal tract, we collected ceca, and distal ileum from 10 3-weeks-old broiler chickens. Lactobacillus strains from cecal lumen contents (M-CL), and those associated with mucosa of ceca (M-CM) and ileum (M-IM) w...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2017.01389
更新日期：2017-07-26 00:00:00
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journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2015.01065
更新日期：2015-10-08 00:00:00
abstract：:Retrospective studies involving the screening of frozen stored collections of samples are commonplace when a new threat emerges, but it has been demonstrated that the freeze-thaw process can affect bacterial viability. The study of colistin-resistant bacteria in human and animal samples is an example of this issue. In...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2020.572712
更新日期：2020-11-09 00:00:00
abstract：:The complexity of the pathogenesis of inflammatory bowel disease (ulcerative colitis and Crohn's disease) has led to the quest of empirically drug therapies, combining immunosuppressant agents, biological therapy and modulators of the microbiota. Helminth parasites have been proposed as an alternative treatment of the...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2018.01036
更新日期：2018-05-23 00:00:00
abstract：:Pathogenic marine bacteria are found in environments and food sources with high salt concentrations, which the bacteria must effectively manage for their survival. Several mechanisms, such as the transport of ions and compatible solutes as well as changes in aerobic and anaerobic respiration, confer salt tolerance to ...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2017.02703
更新日期：2018-01-22 00:00:00
abstract：:Recently, controllable, targeted proteolysis has emerged as one of the most promising new strategies to study essential genes and otherwise toxic mutations. One of the principal limitations preventing the wider adoption of this approach is due to the lack of easily identifiable species-specific degrons that can be use...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2017.02572
更新日期：2017-12-19 00:00:00
abstract：:Cronobacter sakazakii is an opportunistic pathogen that causes severe infections in neonates and infants through contaminated powdered infant formula (PIF). Therefore, the aim of this study was a large-scale study on determine the prevalence, molecular characterization and antibiotic susceptibility of C. sakazakii iso...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2017.02026
更新日期：2017-10-17 00:00:00
abstract：:Nowadays food preservation, quality maintenance, and safety are major growing concerns of the food industry. It is evident that over time consumers' demand for natural and safe food products with stringent regulations to prevent food-borne infectious diseases. Antimicrobial packaging which is thought to be a subset of...
journal_title：Frontiers in microbiology
pub_type： 杂志文章,评审
doi：10.3389/fmicb.2015.00611
更新日期：2015-06-16 00:00:00
abstract：:Bifidobacterium species are used as probiotics to provide beneficial effects to humans. These effects are specific to some species or subspecies of Bifidobacterium. However, some Bifidobacterium species or subspecies are not distinguished because similarity of 16S rRNA and housekeeping gene sequences within Bifidobact...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2020.02087
更新日期：2020-08-28 00:00:00
abstract：:[This corrects the article DOI: 10.3389/fmicb.2020.01776.]. ...
journal_title：Frontiers in microbiology
pub_type： 已发布勘误
doi：10.3389/fmicb.2020.588889
更新日期：2020-10-26 00:00:00
abstract：:Chandipura Virus (CHPV) a negative-stranded RNA virus belonging to the Rhabdoviridae family, has been previously reported to bring about neuronal apoptosis by stimulating oxidative stress. Our in silico data suggested the involvement of Angiotensin II in intracellular Ca2+ secretion within CHPV infected cells that fur...
journal_title：Frontiers in microbiology
pub_type： 杂志文章
doi：10.3389/fmicb.2018.01489
更新日期：2018-07-06 00:00:00
